Vicen_00140 Vicen_00140   Vicen_00160 Vicen_00160

Vicen_00150 : CDS information

close this sectionLocation

Organism
StrainHC34
Entry nameVicenistatin
Contig
Start / Stop / Direction40,886 / 41,869 / + [in whole cluster]
40,886 / 41,869 / + [in contig]
Location40886..41869 [in whole cluster]
40886..41869 [in contig]
TypeCDS
Length984 bp (327 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productalanine-3-amino-2-methylpropionyltransferase
Product (GenBank)malonyl-CoA-[acyl-carrier-protein] transacylase
Gene
Gene (GenBank)vinK
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15112997] Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. (Chem Biol. , 2004)
[22010945] A natural protecting group strategy to carry an amino acid starter unit in the biosynthesis of macrolactam polyketide antibiotics. (J Am Chem Soc. , 2011)
[26831085] Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis. (Proc Natl Acad Sci U S A. , 2016)
comment
[PMID: 15112997](2004)
vicenistatin生合成gene clusterの同定論文。

VinK (327aa) : acyl CoA-ACP transacylase

配列解析からの機能推定。
type II fatty acid synthasesのmalonyl transferaseに相同性あり。
よってstarter acyl unitをACPに移すことを担うと思われる。

---
[PMID: 22010945](2011)
VinJ, K, L, M, N, Oの酵素的解析を行っている論文。

VinP1のACP domainのみを発現し、Sfpでholo型に変換。
alanine-3-amino-2-methylpropionyl-VinL と VinP1-ACPをVinKで反応させ、産物をLC-ESI-MS解析。効率的なdipeptide transferが起こり、alanine-3-amino-2-methylpropionyl-VinP1-ACPが形成された。

また、VinKの基質特異性を確認するため、3つのaminoacyl-VinLs
・3-methylaspartate-VinL
・3-amino-2-methylpropionyl-VinL
・alanine-3-amino-2-methylpropionyl-VinL
を基質として検討。

alanine-3-amino-2-methylpropionyl-VinLのみが選択的にVinP1-ACPへ移動された。

---
[PMID: 26831085](2016)
VinKおよびVinK-VinL複合体の構造解析。
Related Reference
ACC
P0AAI9
PmId
[1314802] Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase. (J Bacteriol. , 1992)
comment
BLAST id27%
E.coli_fabD(tfpA)
Malonyl CoA-acyl carrier protein transacylase(EC 2.3.1.39)

gene cloning(thermosensitive E. coli fabD mutant相補)、精製タンパクで活性みている。

close this sectionPKS/NRPS Module

0
AT46..138

close this sectionSequence

selected fasta
>alanine-3-amino-2-methylpropionyltransferase [malonyl-CoA-[acyl-carrier-protein] transacylase]
MNATVETTQHDVEGTGAAGATAMLFPGMGPAAFSDVGRFMVTNRYTRELLAEADDTLGYS
LVDRFRQAEGDYSEYAQIAFLVNCVALARWAEQTMDLTPRICAGASFGEKSVAAYSGALT
FADAVRMTAGLARCMDEYFRTEHLGVVTHSFVRAPRERLDEILAELDERGEWHEISCHID
HDFFMLTLHERNSVWLEGRLRSVGAMPLYAMRPPMHAAAFGGLRDKAEEEVIAPLTFHDP
TLPVVADQDGKVLTTGDEVRTMLLESFVRPLRWPDVISSLQDQGVTRVCVAGPDSLFGRV
GTTTRAFEVIAATPRLALQPRARTTSR
selected fasta
>alanine-3-amino-2-methylpropionyltransferase [malonyl-CoA-[acyl-carrier-protein] transacylase]
GTGAACGCGACCGTGGAGACGACACAGCACGACGTCGAGGGGACCGGCGCGGCCGGCGCG
ACCGCGATGCTCTTCCCCGGGATGGGACCCGCCGCGTTCTCCGACGTCGGCCGGTTCATG
GTGACCAACCGGTACACCAGGGAACTCCTCGCGGAGGCCGACGACACCCTCGGCTACTCC
CTCGTCGACCGGTTCCGGCAGGCCGAGGGCGACTACTCCGAGTACGCCCAGATCGCCTTC
CTGGTCAACTGCGTCGCCCTCGCCCGCTGGGCCGAGCAGACCATGGACCTCACCCCCCGG
ATCTGCGCCGGAGCCAGCTTCGGCGAGAAGTCCGTCGCCGCCTACAGCGGCGCCCTCACC
TTCGCCGACGCCGTCCGGATGACCGCCGGCCTCGCCCGCTGTATGGACGAGTACTTCCGT
ACGGAGCACCTCGGGGTCGTCACCCACTCCTTCGTCCGCGCCCCTCGGGAGCGGCTCGAC
GAGATCCTCGCCGAACTCGACGAGCGCGGCGAATGGCACGAGATCTCCTGCCACATCGAC
CACGACTTCTTCATGCTCACCCTCCATGAACGCAACAGTGTCTGGCTGGAGGGCCGGCTC
CGGTCGGTGGGCGCCATGCCGCTGTACGCCATGCGGCCCCCGATGCACGCCGCCGCCTTC
GGCGGGCTGCGCGACAAGGCCGAGGAGGAGGTCATCGCCCCCCTCACCTTCCACGACCCG
ACCCTGCCCGTCGTCGCCGACCAGGACGGCAAGGTGCTGACCACCGGCGACGAGGTGCGC
ACCATGCTCCTGGAGAGCTTCGTCCGCCCGCTGCGCTGGCCCGACGTCATCTCCTCGCTC
CAGGACCAGGGCGTCACCCGCGTCTGTGTCGCGGGCCCCGACAGCCTCTTCGGGCGGGTC
GGCACCACCACCCGCGCCTTCGAGGTCATCGCGGCGACCCCGCGGCTCGCCCTCCAGCCC
CGCGCGCGCACCACCTCGCGCTGA
[0] AT46..138
[0] AT136..414

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[19-138] 2.2e-26 G3DSA:3.40.366.10 [213-300] 2.2e-26 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR014043 Acyl transferase (Domain)
[46-138] 1.3e-05 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[20-311] 2.90000354677981e-28 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
SignalP No significant hit
TMHMM No significant hit
Vicen_00140 Vicen_00140   Vicen_00160 Vicen_00160
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