Aver_00070 : CDS information

Location

Organism	Streptomyces avermitilis
Strain	ATCC 31267 (=NBRC 14893)
Entry name	Avermectin
Contig	AB032367
Start / Stop / Direction	39,282 / 37,912 / - [in whole cluster] 33,284 / 31,914 / - [in contig]
Location	complement(37912..39282) [in whole cluster] complement(31914..33284) [in contig]
Type	CDS
Length	1,371 bp (456 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450
Product (GenBank)	cytochrome P450 hydroxylase
Gene
Gene (GenBank)	aveE
EC number
Keyword	furan ring
Note
Note (GenBank)	probabry catalyzes furan ring formation at C6 to C8
Reference	ACC Q9S0R5 PmId [10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999) [7868391] Production of 6,8a-seco-6,8a-deoxy derivatives of avermectins by a mutant strain of Streptomyces avermitilis. (J Antibiot (Tokyo). , 1995) comment [PMID:10449723](1999) avermectin生合成gene clusterの報告。 AveE: Cytochrome P450 hydroxylase PKS aveA4 and aveA3とひとつの転写unitであるかもしれない。 AveEのC末の推定AA配列はcytochrome P450 hydroxylaseのコンセンサス配列に似ていることと既報(Ref26)の相補特性から、AveEはC8aでallylic methyl residueに酸素原子を導入してフラン環閉鎖をするCYP450 hydroxylaseであることが示唆されている。 --- Ref26[PMID: 7868391](1995) derivative strain K2057 (aveD aveE X)が産生したavermectin関連化合物はC-6, C-8aでフラン環を欠いていた。 K2057の相補実験あり。AveEを完全に含むfragmentで強く相補される。

Sequence

>cytochrome P450 [cytochrome P450 hydroxylase]
MMSQSTSSIPEAPGAWPVVGHVPPLMRQPLEFLRSAADHGDLLKLRLGPKTAYLATHPDL
VRTMLVSSGSGDFTRSKGAQGASRFIGPILVAVSGETHRRQRRRMQPGFHRQRLESYVAT
MAAAAQETADSWSAGQVVDVEQAACDLSLAMITKTLFFSDLGAKAEAALRKTGHDILKVA
RLSALAPTLYEVLPTAGKRSVGRTSATIREAITAYRADGRDHGDLLSTMLRATDAEGASM
TDQEVHDEVMGIAVAGIGGPAAITAWIFHELGQNAEIESRLHAELDTVLGGRLPTHEDLP
RLPYTQNLVKEALRKYPGWVGSRRTVRPVRLGGHDLPADVEVMYSAYAIQRDPRWYPEPE
RLDPGRWETKGSSRGVPKGAWVPFALGTYKCIGDNFALLETAVTVAVVASHWRLHALPGD
EVRPKTKATHVFPNRLRMIAEPRSVVRLEEPAAMGA

>cytochrome P450 [cytochrome P450 hydroxylase]
GTGATGTCCCAGTCGACGAGCAGCATTCCCGAGGCGCCCGGCGCCTGGCCGGTCGTCGGC
CATGTGCCGCCGTTGATGCGGCAGCCCCTGGAGTTCCTTCGCTCCGCAGCAGATCACGGA
GACCTGCTCAAGCTCCGCCTCGGGCCGAAAACCGCCTACCTCGCCACGCACCCGGACCTG
GTGCGGACCATGCTGGTCTCCTCCGGATCGGGCGATTTCACCCGGAGCAAGGGCGCACAG
GGCGCGAGCCGCTTCATCGGCCCCATCCTCGTAGCCGTATCGGGGGAGACGCACCGGCGA
CAGCGGAGGCGGATGCAGCCAGGGTTCCACCGCCAGCGCCTCGAGAGCTATGTCGCCACC
ATGGCGGCCGCCGCGCAGGAGACCGCTGATTCCTGGAGCGCTGGGCAGGTGGTGGACGTT
GAGCAGGCCGCCTGCGACCTGTCGCTTGCCATGATCACGAAGACGCTGTTCTTCTCCGAC
CTGGGCGCGAAGGCCGAGGCGGCGCTGCGGAAAACCGGACACGACATTCTGAAGGTGGCC
CGGCTGAGTGCTCTCGCCCCCACCCTTTACGAGGTTCTTCCCACCGCGGGCAAGCGGTCC
GTGGGCCGTACGAGCGCAACGATCCGGGAGGCGATCACGGCGTATCGCGCCGATGGCCGC
GACCACGGTGACCTGCTCTCGACGATGCTGCGCGCGACGGACGCCGAAGGTGCCTCCATG
ACGGACCAGGAGGTCCATGACGAGGTCATGGGAATCGCGGTGGCCGGGATCGGCGGCCCA
GCTGCCATTACGGCGTGGATTTTCCACGAGCTCGGACAGAACGCCGAGATCGAGAGTCGG
CTGCACGCAGAGCTCGACACGGTCCTGGGCGGGCGGTTGCCCACGCACGAGGACCTCCCG
CGGCTGCCGTACACACAGAACCTCGTGAAAGAGGCACTGCGCAAATACCCCGGCTGGGTG
GGGTCCCGCCGGACGGTGCGGCCCGTACGACTCGGCGGTCACGACCTGCCCGCCGATGTG
GAGGTCATGTACAGCGCGTACGCCATCCAGCGCGACCCACGTTGGTACCCCGAACCCGAA
CGTCTCGACCCCGGCCGCTGGGAAACCAAGGGATCGAGCCGCGGAGTTCCCAAGGGGGCG
TGGGTGCCCTTCGCGCTCGGCACCTACAAATGCATCGGCGACAACTTCGCCCTTCTCGAG
ACGGCCGTTACGGTCGCGGTCGTCGCCTCCCACTGGCGACTACACGCCCTTCCGGGCGAC
GAGGTCCGGCCCAAGACGAAGGCCACTCACGTCTTCCCCAACCGACTCCGCATGATCGCT
GAACCGAGGAGTGTGGTGCGTCTGGAGGAGCCCGCCGCCATGGGAGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PR00385 P450 G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 PF00067 p450 IPR002403 Cytochrome P450, E-class, group IV (Family) PR00465 EP450IV
SignalP	No significant hit
TMHMM	No significant hit

Aver_00060 Aver_00080