Adria_00210 : CDS information

Location

Organism	Streptomyces peucetius
Strain	ATCC 29050 (=NBRC 100596)
Entry name	Doxorubicin
Contig	M80237
Start / Stop / Direction	16,804 / 15,986 / - [in whole cluster] 202 / 1,020 / + [in contig]
Location	complement(15986..16804) [in whole cluster] 202..1020 [in contig]
Type	CDS
Length	819 bp (272 aa)

Annotation

Category	4.1 transcriptional regulator
Product	transcriptional activator
Product (GenBank)
Gene
Gene (GenBank)	dnrI
EC number
Keyword	SARP family
Note
Note (GenBank)
Reference	ACC P25047 PmId [1729206] Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius. (J Bacteriol. , 1992) [7868593] Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995) [8971703] Purification and characterization of the DNA-binding protein DnrI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius. (Mol Microbiol. , 1996) [16545946] Feedback regulation of doxorubicin biosynthesis in Streptomyces peucetius. (Res Microbiol. , 2006) [20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) comment [PMID: 1729206](1992) ATCC 29050 strainでdnrI を不活化したら、RHO産生とdaunorubicin (DNR)産生ができなくなり、DNRへの抵抗性が著しく減った。 --- 同じ著者らの続報。 [PMID: 7868593](1995) dnrI::aphII mutantでは、DNR生合成におけるearly- and late-acting enzymesの両方をコードする転写物が存在しない。よってdnrI は、直接または間接的にDNR生合成遺伝子のすべてを調節している。 dnrI::aphII and dnrN::aphII strainsでresistance genes=drrAB and drrC転写物は消失。よって、dnrI はDNR抵抗性の重大なregulatorであり、dnrNはdnrI を通して間接的にその影響を発現する、と示唆される。 -- [PMID: 8971703](1996) abstract DNA-binding assays. ・dnrG-dpsABCD と dpsEF の間・dnrC gene と dnrDKPSQ の間の遺伝子間領域を含んだDNA断片へ特異的に結合する。 -- [PMID: 16545946](2006) abstract dnrI promoter活性を増やすためにはDnrO and DnrN の両方が必要であった。 -- [PMID: 20529134]や[PMID: 20158521]で、dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。 dnrI promoterにはDnrNが結合し活性化される。

Sequence

>transcriptional activator
MQINMLGPLVAHHNGTSVTPIARKPRQVFSLLALQAGTVVPVPALMDELWGTQPPASALT
TLQTYILQVRRGITVALGASHNGPAKDVLRTCYGGYLLDVDPTNTDVYAFERLAEEGKRA
CERGELDLASARFRQALDLWRGDALVDVHAGMRIGMEVARLEESRLGVLEARMETDLRLG
RHAGLLPELSALTARHPMHENLWAQFMIALHRSGRTSQALEAFIKLRKTLVNELGVEPSA
RLQHLQHAILRADPGIDRNGPEVPAAASVALA

>transcriptional activator
ATGCAGATCAATATGTTGGGCCCGCTCGTCGCACATCACAATGGCACGTCGGTGACCCCG
ATAGCCAGAAAGCCCCGGCAAGTATTCTCACTGCTCGCTCTCCAGGCAGGAACCGTCGTG
CCGGTGCCCGCGCTGATGGACGAGCTCTGGGGAACCCAACCCCCGGCGAGCGCGCTGACC
ACCCTTCAGACCTACATCCTTCAAGTGCGCCGGGGCATCACCGTGGCCCTCGGCGCGAGC
CACAACGGACCCGCCAAGGACGTGCTGCGCACCTGCTACGGCGGCTACCTGCTCGACGTG
GACCCCACCAACACCGACGTGTACGCCTTCGAGCGTCTCGCCGAGGAGGGCAAGCGGGCG
TGCGAGCGGGGAGAGCTCGACCTGGCCTCCGCACGCTTCCGCCAGGCCCTCGACCTGTGG
CGCGGAGACGCGCTCGTGGACGTGCACGCCGGCATGCGGATCGGCATGGAGGTGGCGCGC
CTGGAGGAGAGCCGGCTCGGCGTCCTGGAGGCCCGCATGGAGACCGACCTGAGGCTGGGC
CGGCACGCGGGCCTGCTGCCCGAGCTGTCGGCGCTCACCGCCCGCCACCCCATGCACGAG
AACCTGTGGGCCCAATTCATGATCGCCCTCCATCGCTCGGGCCGCACGAGCCAGGCCCTC
GAAGCCTTCATCAAACTCCGCAAGACATTGGTGAACGAGCTGGGGGTGGAACCGTCCGCC
CGTCTCCAGCACCTCCAGCACGCGATTCTGCGGGCCGACCCCGGCATCGACCGGAACGGT
CCGGAAGTCCCGGCAGCCGCGTCCGTCGCGCTTGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001867 Signal transduction response regulator, C-terminal (Domain) SM00862 Trans_reg_C PF00486 Trans_reg_C IPR005158 Bacterial transcriptional activator domain (Domain) PF03704 BTAD SM01043 BTAD IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Adria_00220 Adria_00010