Adria_00240 Adria_00240   Adria_00260 Adria_00260

Adria_00250 : CDS information

close this sectionLocation

Organism
StrainATCC 29050 (=NBRC 100596)
Entry nameDoxorubicin
Contig
Start / Stop / Direction26,670 / 25,885 / - [in whole cluster]
2,680 / 1,895 / - [in contig]
Locationcomplement(25885..26670) [in whole cluster]
complement(1895..2680) [in contig]
TypeCDS
Length786 bp (261 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productpolyketide C-9 ketoreductase
Product (GenBank)daunorubicin-doxorubicin polyketide synthase
Gene
Gene (GenBank)dpsE
EC number
Keyword
Note
Note (GenBank)
  • protein E; putative
Reference
ACC
PmId
[7828855] Characterization of the Streptomyces peucetius ATCC 29050 genes encoding doxorubicin polyketide synthase. (Gene. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
[PMID: 7828855](1994)
daunorubicin (DNR)-doxorubicin (DXR) PKSを含む領域9ORFsの同定論文。

dpsE: ketoreductase
配列解析のみ。たぶん下流dpsFとoperon形成。

--
[PMID: 9224566](1997)
DpsEは、DpsFによる環化に先立って必要なC-9還元を担う、decaketideに特異的なketoreductaseであることが実験で確認されている。

minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。KRとして、dpsE, jadE, actIII を使用している。

DpsEはdecaketidesを還元できるが、octaketidesを還元できない。
JadEはdecaketideだけでなく、octaketideも還元できる。

JadD, DpsFの両cyclasesはC-9で還元されたdecaketidesの位置特異的なfirst-ring cyclase/dehydrasesである。

close this sectionSequence

selected fasta
>polyketide C-9 ketoreductase [daunorubicin-doxorubicin polyketide synthase]
MSEAADRVALVTGGTSGIGLAVVRKLAQDGTRVFLCARDESAITGTVKELQASGLEVDGA
PCDVRSTADVDRLVQTARNRFGPIDIVVNNAGRGGGGVTAEITDDLWLDVVDTNLSGAFR
VTRAVLTGGAMQEHGWGRIISIASTGGKQGVALGAPYSASKSGLIGFTKAVALELAKTGI
TVNAVCPGYVETPMAQGVRQRYAAFWGITEDDVLEKFQAKIPLGRYSMPEEVAGMVHYLA
SDSADSITAQAINVCGGLGSY
selected fasta
>polyketide C-9 ketoreductase [daunorubicin-doxorubicin polyketide synthase]
ATGTCCGAGGCAGCCGACCGGGTCGCACTCGTCACCGGGGGAACCAGCGGCATCGGTCTC
GCGGTGGTGCGCAAGCTCGCCCAGGACGGGACCCGGGTGTTCCTGTGCGCTCGTGACGAG
AGCGCCATCACCGGCACCGTGAAGGAACTTCAGGCATCGGGACTGGAGGTGGACGGCGCG
CCCTGCGACGTGCGGAGCACCGCCGACGTCGACCGGCTGGTCCAAACGGCCCGGAACCGC
TTCGGGCCGATCGACATCGTCGTCAACAACGCCGGCCGCGGCGGTGGAGGCGTGACCGCC
GAGATCACGGACGACCTGTGGCTCGACGTCGTCGACACCAACCTCAGCGGGGCCTTCCGG
GTCACCCGCGCCGTCCTCACCGGCGGCGCCATGCAGGAGCACGGCTGGGGCCGGATCATC
AGCATCGCCTCCACCGGCGGCAAGCAGGGAGTGGCCCTCGGCGCCCCCTACTCCGCCTCC
AAGAGCGGCCTGATCGGCTTCACGAAGGCCGTCGCGCTGGAGCTGGCCAAGACGGGCATC
ACCGTCAACGCCGTCTGCCCCGGGTACGTCGAGACGCCCATGGCACAGGGCGTGCGGCAG
CGCTACGCCGCCTTCTGGGGGATCACCGAGGACGACGTCCTGGAGAAGTTCCAGGCGAAG
ATCCCCCTCGGACGCTACTCGATGCCCGAGGAGGTGGCCGGGATGGTGCACTACCTGGCC
TCCGACAGCGCCGACTCGATCACGGCCCAGGCCATCAACGTCTGTGGTGGCCTCGGCAGC
TACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[82-93] 2.39999798157265e-13 PR00080 [137-145] 2.39999798157265e-13 PR00080 [157-176] 2.39999798157265e-13 PR00080
PR00080   SDRFAMILY
[8-175] 1.99999999999999e-38 PF00106
PF00106   adh_short
[3-248] 1.89999859865864e-15 PIRSF000126
PIRSF000126   11-beta-HSD1
IPR002347 Glucose/ribitol dehydrogenase (Family)
[8-25] 1.99999636034521e-48 PR00081 [82-93] 1.99999636034521e-48 PR00081 [131-147] 1.99999636034521e-48 PR00081 [157-176] 1.99999636034521e-48 PR00081 [178-195] 1.99999636034521e-48 PR00081 [222-242] 1.99999636034521e-48 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[3-258] 1e-82 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[144-172] PS00061
PS00061   ADH_SHORT
SignalP No significant hit
TMHMM No significant hit
Adria_00240 Adria_00240   Adria_00260 Adria_00260
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