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Adria_00370 : CDS information

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Organism
StrainATCC 29050 (=NBRC 100596)
Entry nameDoxorubicin
Contig
Start / Stop / Direction41,213 / 38,919 / - [in whole cluster]
991 / 3,285 / + [in contig]
Locationcomplement(38919..41213) [in whole cluster]
991..3285 [in contig]
TypeCDS
Length2,295 bp (764 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
ProductUvrA-like protein
Product (GenBank)daunorubicin resistance protein
Gene
Gene (GenBank)drrC
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[8655504] The Streptomyces peucetius drrC gene encodes a UvrA-like protein involved in daunorubicin resistance and production. (J Bacteriol. , 1996)
[9835035] The DrrC protein of Streptomyces peucetius, a UvrA-like protein, is a DNA-binding protein whose gene is induced by daunorubicin. (FEMS Microbiol Lett. , 1998)
comment
[PMID: 8655504](1996)
DrrCはDNAの除去修復に関連するE.coli_UvrAに強いAA配列類似あり。
S.lividansへのdrrC導入はdaunorubicin (DNR)抵抗性を与える。
DNR and doxorubicin (DXR) antiporterによって抵抗性をもたらすDrrABのシステムと異なり、たぶんゲノムDNAへのDNR and DXRの結合を抑制または不安定化をしうる。

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同じ著者らの続報
[PMID: 9835035](1998)
E.coliで発現して得たDrrCは、ATPとDNRの存在下でのみ、DNR産生遺伝子のpromoter領域を含むDNA segmentを結合。
S.peucetiusにおけるDrrCの出現はDNR産生の開始と同時に起こり、drrC geneはDNRによって誘導される。
DnrN and DnrI regulatory proteinsはdrrC発現のために必要。
DrrAは、DrrCと同じ様式で調節された。

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参考[PMID: 20158521](2010)
この論文での実験にdrrCは絡んでいないが、総合的なFigあり。

細胞内DNR濃度が上昇したときに、DNR biosynthetic genes and resistance genesのactivatorであるDnrIのpromoter領域へfeedback阻害のために結合したDNR（本来はDnrNが結合）を、再活性化のために離すことに関連しているのではないかと言っている。

close this sectionSequence

selected fasta
>UvrA-like protein [daunorubicin resistance protein]
MQQQAIQIIGARENNLKGISVEVPKHKVTVFTGVSGSGKSSLVFDTIAAESQRQLNETFT
AFVRNRLPKYGQPDVDDIANLSAAVIIDQKRIGGNARSTVGTITDIYALLRLLYSRVGKP
WIGYSNAFSFNDPSGMCPDCEGLGHRVQVDLDKLLDRSKSLNEGAIRHPAFNVGGWFWKL
YANSGLFDNDKKLRDYTESEWQAFLYGAQGSIALEWQGGKVNSKYEGLMDKFNRLYLRKE
PDEMSAKNRVALQQVVTRGPCDTCKGQRLSQRALGCRINGRNIAELASLEIADLMEAVKK
VDTSSVSTLTASLIERLQHLIELGLGYLSLNRETSTLSGGESQRIKMVRHLNSSLVDMVY
IFDEPTIGLHPSDVQRLSRLLMELAEKGNTVLVVEHDRDIIEVAEHVIDIGPGAGRHGGE
IVYQGDVRGLTEADTPTGRFLRRIIPIKERFRGPRGHLEVTGARIHNLKDISVRIPLGVL
TAVTGPAGSGKSTLIHDVLLRQHPSAVVIDQSAVTTNRRSNPATYTGIMDEIRRLFAREN
QVSPSLFSFNSEGACPTCQGHGIIYTDLAFMDPMITTCEVCAGMRFTDDVLRLTLRGQNI
HDVLSMSAAEAAEFFTEPKVAKTLRSINQVGLGYLQLGQPLNTLSGGECQRIKLATELGK
KGSVYVMDEPTTGLHMSDVDSLVHLVDGLVEKGDSVIVIEHNLDVVKHADWVVDLGPGGG
SEGGRVIFEGTPADLAEAEHSVTGRFLAASLAGAGSEADHRARR
selected fasta
>UvrA-like protein [daunorubicin resistance protein]
ATGCAGCAGCAGGCTATTCAGATCATCGGTGCCCGTGAGAACAACCTCAAGGGCATCTCC
GTGGAGGTGCCGAAACACAAGGTCACCGTGTTCACGGGGGTATCGGGATCGGGAAAGTCG
TCCTTGGTATTCGACACAATCGCGGCGGAATCCCAACGGCAGCTCAATGAGACGTTCACC
GCGTTCGTGCGCAATCGCCTGCCCAAATACGGTCAGCCCGACGTGGACGACATCGCGAAC
CTCTCCGCTGCGGTGATCATCGATCAGAAGCGGATCGGGGGGAACGCGCGGTCCACCGTC
GGGACGATCACCGACATCTACGCCTTGCTCCGGCTGCTCTATTCCCGGGTGGGCAAGCCC
TGGATTGGTTATTCGAACGCCTTCTCGTTCAACGACCCCTCGGGAATGTGCCCCGATTGC
GAGGGCCTCGGCCACCGGGTGCAGGTCGACCTCGACAAGCTGCTCGACAGGTCCAAGTCC
CTCAATGAGGGCGCCATCCGGCATCCCGCCTTCAATGTTGGTGGCTGGTTCTGGAAGCTG
TACGCCAATTCCGGCCTCTTCGACAACGACAAGAAGCTCCGCGACTACACCGAGAGCGAG
TGGCAGGCGTTCCTGTACGGGGCCCAGGGGTCGATCGCTCTGGAGTGGCAGGGTGGCAAG
GTCAACTCGAAGTACGAAGGGCTGATGGACAAGTTCAACCGGCTCTACCTGCGCAAGGAG
CCGGACGAGATGAGCGCCAAGAACCGGGTGGCGCTGCAACAGGTCGTCACCCGGGGGCCC
TGCGACACCTGCAAGGGGCAACGGCTCAGCCAGCGGGCGCTCGGCTGCCGCATCAACGGC
CGGAACATCGCCGAACTCGCCTCCCTGGAGATCGCCGACCTGATGGAGGCGGTCAAGAAG
GTCGACACGTCCTCCGTCTCCACCCTGACGGCCAGTCTGATCGAGCGGCTCCAGCACCTC
ATCGAACTGGGGCTGGGATACCTCAGCCTCAACCGTGAGACCTCCACGCTCTCCGGCGGC
GAGTCGCAGCGCATCAAGATGGTGCGTCACCTGAACAGTTCGCTCGTCGACATGGTGTAC
ATCTTCGACGAGCCCACGATCGGCCTGCACCCCAGCGACGTCCAGCGGCTCAGCCGGCTC
CTGATGGAACTGGCCGAGAAGGGCAACACCGTGCTGGTGGTCGAGCACGACCGCGACATC
ATCGAGGTGGCCGAGCACGTCATCGACATCGGGCCCGGGGCCGGGCGGCACGGAGGCGAG
ATCGTCTACCAGGGCGACGTCCGGGGCCTCACCGAGGCGGACACGCCGACCGGACGGTTC
CTCCGCAGGATCATCCCGATCAAGGAGCGATTCCGCGGGCCCAGGGGACACCTGGAGGTG
ACGGGAGCCCGGATCCACAACCTCAAGGACATCAGCGTACGGATTCCGCTCGGCGTACTG
ACCGCCGTCACCGGACCGGCTGGCTCCGGCAAGAGCACGCTCATCCACGACGTACTGCTG
CGGCAGCACCCCTCGGCGGTCGTCATCGACCAGTCCGCGGTCACCACCAACCGGCGTTCG
AACCCGGCGACCTACACCGGCATCATGGACGAGATCCGCAGGCTGTTCGCCCGCGAGAAC
CAGGTCAGCCCCTCACTGTTCAGCTTCAACTCCGAGGGCGCCTGCCCCACCTGCCAGGGA
CACGGCATCATCTACACCGACCTCGCCTTCATGGATCCGATGATCACGACCTGCGAGGTC
TGCGCGGGGATGCGTTTCACCGATGACGTCCTGCGCCTGACCCTGCGCGGGCAGAACATC
CACGACGTGCTGTCGATGAGCGCCGCTGAGGCGGCCGAGTTCTTCACCGAACCCAAGGTT
GCCAAGACCCTCCGTTCCATCAACCAGGTCGGCCTCGGCTATCTCCAGCTCGGGCAGCCG
CTCAACACCCTCTCCGGCGGCGAGTGCCAGCGCATCAAGCTCGCCACGGAGCTTGGGAAG
AAGGGCAGCGTCTACGTCATGGACGAGCCCACCACCGGACTCCACATGTCCGACGTCGAC
AGCCTCGTACACCTGGTCGACGGCCTCGTCGAGAAGGGGGACTCGGTCATCGTCATCGAG
CACAATCTCGACGTCGTCAAGCATGCGGACTGGGTCGTCGACCTCGGCCCCGGGGGCGGC
AGCGAGGGCGGCCGGGTGATCTTTGAGGGCACCCCCGCTGACCTGGCGGAGGCCGAGCAC
TCCGTCACTGGGCGGTTCCTCGCCGCGTCCCTGGCGGGCGCCGGTTCCGAGGCCGACCAC
CGGGCCCGGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003439 ABC transporter-like (Domain)
[316-366] 5.60000000000001e-05 PF00005 [619-672] 2.3e-09 PF00005
PF00005   ABC_tran
[1-437] PS50893 [445-742] PS50893
PS50893   ABC_TRANSPORTER_2
IPR003593 AAA+ ATPase domain (Domain)
[25-414] 0.00220000021756082 SM00382 [477-753] 0.000129999995835436 SM00382
SM00382   AAA
IPR017871 ABC transporter, conserved site (Conserved_site)
[644-658] PS00211
PS00211   ABC_TRANSPORTER_1
SignalP No significant hit
TMHMM No significant hit
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