Resis_00050 : CDS information

Location

Organism	Streptomyces resistomycificus
Strain	HKI-237
Entry name	Resistomycin
Contig	AJ585192
Start / Stop / Direction	3,023 / 4,066 / + [in whole cluster] 3,023 / 4,066 / + [in contig]
Location	3023..4066 [in whole cluster] 3023..4066 [in contig]
Type	CDS
Length	1,044 bp (347 aa)

Annotation

Category	5.1 general function
Product	putative methyltransferase
Product (GenBank)	RemH protein
Gene
Gene (GenBank)	remH
EC number	2.1.1.-
Keyword
Note	This ORF is not involved in resistomycin biosynthesis.
Note (GenBank)
Reference	ACC Q70DX3 PmId [14982421] A gene cluster encoding resistomycin biosynthesis in Streptomyces resistomycificus; exploring polyketide cyclization beyond linear and angucyclic patterns. (J Am Chem Soc. , 2004) [17914826] Geminal tandem C-methylation in the discoid resistomycin pathway. (J Am Chem Soc. , 2007) comment [PMID:14982421](2004) resistomycin生合成gene cluster全体を異種性発現により同定。 RemH: Methyltransferase PKS後の修飾として、bis-nor-resistomycinはgeminal bis-methylationを受ける。既報の13C-labeling実験でresistomycinの両方のgem methyl基がS-adenosyl methionine(SAM)に由来することがわかっている。remG, remHにはprobable SAM-binding methylTFに特徴的なmotifが保存されているので、この反応に関与することが示唆される。 --- [PMID: 17914826](2007) delta-remGH, delta-remH, delta-remGを作成。相補実験もしている。 delta-remGHはresistomycin非産生。remGH、remGで相補可能だがremHでは相補不可。 delta-remGもresistomycin非産生。remGで相補可能。 delta-remHはresistomycin産生可能。 remGを欠いたmutantでは不安定な化合物2を検出。この化合物2がgeminal bis-methylationを欠いている構造であることを確認。よってRemGのみがresistomycinに2つのmethyl基を導入するのに必要とされる。
Related Reference	ACC Q70DX4 NITE Resis_00040 PmId [17914826] Geminal tandem C-methylation in the discoid resistomycin pathway. (J Am Chem Soc. , 2007) comment Blast 2nd, id34%, 5e-25 rem cluster内homolog RemG

Sequence

>putative methyltransferase [RemH protein]
MTDTPVRAAPPASPAAALARYTTAPDPGPLLALNFGFARARALGTALELGVFTALARRPR
GTEDLAAALGCDPRALGDLLAVLTEFGLLTGDGQGLTELSRAYLVEGEPTYLGDHFAEVL
GQWDRWSALTPVTRSGDRSGDFGAPAARGRHPGLFGGGFPLAVRVAFAAVEALGLRRAGR
VLDLACGGGEWGIALAAADPRAEVTAHDDPVLLGSARARAAEFGVADRFRFVSADFAAPP
FADAGFDTVVLAHAGRFAGPTATARLVRECARQLRPGGTLLVADVMRPEPGRPALSRPML
GLSLLVNTADGAVLAAQDYRALMEDAGVTAKECVTRGLVTAQTGKRR

>putative methyltransferase [RemH protein]
ATGACGGACACCCCGGTGCGGGCCGCGCCGCCCGCCTCCCCCGCAGCGGCCCTCGCCCGC
TACACGACCGCCCCCGACCCGGGCCCGCTGCTCGCCCTCAACTTCGGCTTCGCCAGGGCC
AGAGCCCTCGGTACGGCCCTGGAACTGGGCGTCTTCACCGCGCTGGCACGGCGCCCACGC
GGCACCGAGGACCTCGCCGCCGCCCTCGGCTGCGACCCCCGGGCCCTGGGCGACCTGCTG
GCCGTCCTCACCGAGTTCGGACTGCTCACCGGTGACGGGCAGGGTCTGACCGAGCTCTCC
CGCGCCTACCTCGTCGAGGGCGAACCCACCTACCTGGGCGACCACTTCGCCGAGGTGCTC
GGCCAGTGGGACCGGTGGAGCGCGCTGACCCCGGTCACCCGCTCCGGCGACCGCTCCGGG
GACTTCGGCGCCCCCGCCGCGCGCGGCCGCCACCCCGGACTGTTCGGCGGCGGTTTCCCG
CTCGCCGTCCGGGTCGCCTTCGCGGCCGTCGAGGCCCTGGGGCTGCGCCGGGCGGGCCGG
GTGCTGGACCTGGCCTGCGGGGGCGGCGAGTGGGGCATCGCCCTGGCCGCCGCCGATCCG
CGGGCCGAGGTGACCGCGCACGACGATCCGGTGCTGCTCGGGTCGGCCCGCGCGCGGGCG
GCGGAGTTCGGGGTGGCCGACAGGTTCCGGTTCGTGTCCGCCGACTTCGCCGCTCCGCCG
TTCGCCGACGCCGGTTTCGACACCGTGGTGCTGGCGCACGCGGGCCGCTTCGCCGGGCCC
ACGGCCACGGCCCGGCTGGTGCGGGAGTGCGCCCGGCAGCTCCGCCCCGGCGGCACCCTG
CTGGTGGCCGACGTGATGCGGCCGGAGCCGGGGCGGCCCGCGCTGAGCCGGCCGATGCTC
GGGCTCAGCCTTCTGGTCAACACCGCGGACGGGGCCGTACTCGCCGCGCAGGACTACCGC
GCCCTCATGGAGGACGCCGGTGTGACGGCGAAGGAATGCGTAACGCGCGGGCTGGTGACG
GCCCAGACAGGGAAAAGGCGGTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd
SignalP	No significant hit
TMHMM	No significant hit

Resis_00040 Resis_00060