Resis_00050 Resis_00050   Resis_00070 Resis_00070

Resis_00060 : CDS information

close this sectionLocation

Organism
StrainHKI-237
Entry nameResistomycin
Contig
Start / Stop / Direction4,069 / 5,355 / + [in whole cluster]
4,069 / 5,355 / + [in contig]
Location4069..5355 [in whole cluster]
4069..5355 [in contig]
TypeCDS
Length1,287 bp (428 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)RemA protein
Gene
Gene (GenBank)remA
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[14982421] A gene cluster encoding resistomycin biosynthesis in Streptomyces resistomycificus; exploring polyketide cyclization beyond linear and angucyclic patterns. (J Am Chem Soc. , 2004)
[18533655] Orchestration of discoid polyketide cyclization in the resistomycin pathway. (J Am Chem Soc. , 2008)
comment
[PMID:14982421](2004)
resistomycin生合成gene cluster全体を異種性発現により同定。

RemA: Ketosynthase KS-alpha
機能推定はAA配列相同性から。

---
[PMID: 18533655](2008)
minimal rem PKSを検証。

remABDCI をS.lividans TK23で発現しても十分な量のpolyketide得られず。
delta-remABDCI と remABDCI のplasmidをS.lividans TK23で同時に発現すると、resistomycin産生が得られる。delta-remABDCI は、tetracenomycin生合成遺伝子の対応するgenes tcmJKLMNで相補されresistomycinを産生できる。

よって、直線状decaketide(tetracenomycin)と円盤状decaketide(resistomycin)の初期経路は同一であると言っている。

remABDCI + remFGLでresistomycin産生可能。
Related Reference
ACC
Q54173
NITE
Urd_00120
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 12th, id44%, 3e-92
Streptomyces fradiae_urdA
Putative ketoacyl synthase

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdAはPKS gene clustersのbeta-ketoacyl synthasesに高い類似性あり。
UrdAとUrdBはid36%で似ているが、UrdBにはないactive site cysteineをUrdAは持っている。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

KS1..380

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [RemA protein]
MREVVVTGLGVISPAGIGKEAFWDTVSAGKSVTKSLSDITSSSLFKSFEFASDAIAEVPG
FEEQIPSLPPEVQRLDRYVQFAVAAALQAVRDAGLQDGGHDAERTGISLSTAICGTPRME
EEFLAVTDEGRRPIDPAKAGRDLYLAAMSNTPGTVLSALLGAQGPCVTLSTGCIGGIDAV
GHAYESIAHGDADVMIAGASEAPITPVTVASFEIINCLSRAHRGHPEAASRPYDADRDGF
VLGEGCGIVVLEEKEHALRRGATPYLQISGFSHTSNAIHMTDLLSDGEDLTRAMSQAIEV
SGVAPGAIDHVSSHGSSTRQNDTCETSALKLALGERAREIPVNSAKSMLGHALAAASAME
IVLCALAGRHSFVHPTANYETPDPTCDLDYVPGTGRPWDGEVILKDASGFAGLHAAMVAR
TVRDGGRS
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [RemA protein]
ATGCGAGAAGTGGTCGTCACCGGGCTAGGCGTGATCAGCCCGGCGGGAATCGGGAAAGAA
GCCTTCTGGGACACGGTCTCGGCGGGAAAAAGCGTCACGAAATCCCTTTCCGACATCACG
AGTTCCTCCCTTTTCAAGTCCTTCGAATTCGCCTCCGACGCGATAGCCGAGGTCCCCGGA
TTCGAGGAGCAGATACCTTCGCTGCCACCGGAGGTGCAACGCCTCGACCGGTACGTGCAG
TTCGCGGTCGCGGCCGCACTGCAGGCCGTCCGTGACGCGGGGCTCCAGGACGGCGGCCAC
GACGCCGAGCGCACCGGGATCTCCCTGTCCACGGCGATCTGCGGCACGCCCAGGATGGAG
GAGGAGTTCCTCGCCGTCACCGACGAGGGCCGGAGGCCCATCGACCCGGCCAAGGCGGGC
CGCGACCTGTATCTCGCGGCCATGTCGAACACGCCCGGGACCGTCCTGTCGGCCCTGCTC
GGTGCCCAGGGCCCCTGCGTCACCCTCTCCACGGGCTGCATCGGCGGCATCGACGCGGTC
GGCCACGCCTACGAGTCGATCGCCCACGGCGACGCCGACGTCATGATCGCGGGTGCCAGC
GAGGCCCCCATCACCCCGGTGACCGTGGCCTCCTTCGAGATCATCAACTGCCTCTCCCGC
GCCCACCGGGGCCACCCCGAGGCCGCCTCGCGCCCCTACGACGCGGACCGCGACGGCTTC
GTCCTCGGCGAGGGCTGCGGCATCGTCGTCCTGGAGGAGAAGGAACACGCGCTGCGCCGC
GGCGCCACCCCCTACCTCCAGATCAGCGGCTTCTCGCACACGTCCAACGCCATCCACATG
ACCGACCTGCTGTCCGACGGCGAGGACCTGACCCGGGCGATGAGCCAGGCCATCGAGGTC
TCCGGGGTGGCGCCCGGCGCGATCGACCACGTCAGCTCGCACGGCAGCTCCACCCGGCAG
AACGACACCTGCGAGACCAGCGCCCTCAAGCTGGCGCTCGGCGAGCGCGCCCGGGAGATC
CCGGTGAACTCGGCGAAGTCCATGCTCGGCCATGCCCTGGCCGCCGCCAGCGCCATGGAG
ATCGTGCTGTGCGCCCTGGCCGGCCGGCACTCCTTCGTGCACCCCACGGCCAACTACGAG
ACGCCCGACCCCACCTGCGACCTCGACTACGTGCCGGGCACCGGCCGTCCCTGGGACGGC
GAGGTCATCCTCAAGGACGCCAGCGGCTTCGCGGGCCTGCACGCCGCGATGGTCGCCCGG
ACCGTCCGGGACGGGGGCCGGTCATGA
KS1..380
KS1..1140

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[1-257] 2.99999999999998e-44 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[266-380] 9.20000000000002e-26 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[1-269] 1.9e-53 G3DSA:3.40.47.10 [274-422] 3.5e-42 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[1-262] 2.69998997787809e-59 SSF53901 [224-422] 7.60000011115808e-54 SSF53901
SSF53901   Thiolase-like
SignalP No significant hit
TMHMM No significant hit
Resis_00050 Resis_00050   Resis_00070 Resis_00070
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