Resis_00060 Resis_00060   Resis_00080 Resis_00080

Resis_00070 : CDS information

close this sectionLocation

Organism
StrainHKI-237
Entry nameResistomycin
Contig
Start / Stop / Direction5,352 / 6,590 / + [in whole cluster]
5,352 / 6,590 / + [in contig]
Location5352..6590 [in whole cluster]
5352..6590 [in contig]
TypeCDS
Length1,239 bp (412 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase chain length factor subunit
Product (GenBank)RemB protein
Gene
Gene (GenBank)remB
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[14982421] A gene cluster encoding resistomycin biosynthesis in Streptomyces resistomycificus; exploring polyketide cyclization beyond linear and angucyclic patterns. (J Am Chem Soc. , 2004)
[18533655] Orchestration of discoid polyketide cyclization in the resistomycin pathway. (J Am Chem Soc. , 2008)
comment
[PMID:14982421](2004)
resistomycin生合成gene cluster全体を異種性発現により同定。

RemB: Ketosynthase KS-beta
機能推定はAA配列相同性から。

---
[PMID: 18533655](2008)
minimal rem PKSを検証。

remABDCI をS.lividans TK23で発現しても十分な量のpolyketide得られず。
delta-remABDCI と remABDCI のplasmidをS.lividans TK23で同時に発現すると、resistomycin産生が得られる。delta-remABDCI は、tetracenomycin生合成遺伝子の対応するgenes tcmJKLMNで相補されresistomycinを産生できる。

よって、直線状decaketide(tetracenomycin)と円盤状decaketide(resistomycin)の初期経路は同一であると言っている。

remABDCI + remFGLでresistomycin産生可能。
Related Reference
ACC
Q54174
NITE
Urd_00130
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 23rd, id38%, 1e-58
Streptomyces fradiae_urdB
Putative chain length determinant

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdBは芳香族PKS gene clustersのCLFに似ている。
UrdAとUrdBはid36%で似ているが、UrdBはactive site cysteineを持っていない。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

CLF2..370

close this sectionSequence

selected fasta
>polyketide synthase chain length factor subunit [RemB protein]
MSIVITGIGAVTPAGDGVEPLWNSAREGTSHLATIDRFDAGDYACPVAGQADFDADAELP
SRFVKRTDRFTHLAVHTVRRALADAGVAVGTDVSPERTGVMVGNILGGWEFAERELRKLW
TDGPRAVSPYQATAWFPAAPQGNICIDQGIKGPARTFVADRASGAYALIGAAETLLRGRA
DVVLAGGVEAPLSPYGWLCLETSGLVAKARGSLAPHELYRPFDAGHGGSVFAEGSVFLVM
ERREHAERRGARVLGELAGWGVSTDGYMPYYTVEPTGRVLGRAMRTATERAGISDGDLGA
VFAHGSGIPFEDVTEGYGLWEAFGPGAARVPVTAPKSGFGHLLGAAAPADVALALRAMAA
GSVPPTVNLDRQAPGIELDIVRGAARPAPEWEHTLVVSRGLGGVNACLLLRR
selected fasta
>polyketide synthase chain length factor subunit [RemB protein]
ATGAGCATCGTCATCACCGGCATCGGTGCGGTCACCCCGGCGGGCGACGGCGTCGAACCC
CTGTGGAACAGCGCCCGCGAGGGCACCTCGCACCTCGCCACGATCGACCGCTTCGACGCC
GGGGACTACGCCTGCCCCGTCGCCGGCCAGGCCGACTTCGACGCCGACGCCGAGCTGCCC
TCCCGCTTCGTCAAGCGCACCGACCGCTTCACCCACCTCGCCGTGCACACCGTGCGCCGG
GCCCTGGCGGACGCCGGGGTCGCCGTCGGCACCGACGTCAGCCCGGAGCGCACCGGCGTC
ATGGTCGGCAACATCCTCGGCGGCTGGGAGTTCGCCGAGCGCGAGCTGCGCAAGCTGTGG
ACCGACGGCCCCCGCGCGGTCAGCCCGTACCAGGCCACCGCCTGGTTCCCGGCGGCCCCG
CAGGGCAACATCTGCATCGATCAGGGCATCAAGGGCCCGGCCCGCACGTTCGTCGCCGAC
CGGGCCAGCGGTGCCTACGCGCTGATCGGCGCCGCCGAGACCCTGCTGCGCGGCCGGGCG
GACGTCGTCCTCGCGGGTGGGGTGGAGGCCCCGCTGTCGCCGTACGGCTGGCTGTGCCTG
GAGACCAGCGGGCTGGTCGCCAAGGCCCGCGGCTCCCTCGCCCCGCACGAGCTGTACCGG
CCCTTCGACGCCGGGCACGGCGGCAGCGTCTTCGCCGAGGGCTCGGTGTTCCTGGTGATG
GAGCGCCGTGAGCACGCCGAGCGGCGCGGCGCCCGCGTCCTCGGCGAACTCGCCGGCTGG
GGCGTCAGCACCGACGGCTACATGCCGTACTACACGGTCGAGCCGACCGGCCGGGTGCTG
GGCCGGGCCATGCGCACCGCCACCGAGCGCGCCGGGATCTCCGACGGCGACCTGGGCGCG
GTCTTCGCACACGGCTCCGGCATCCCCTTCGAGGACGTCACCGAGGGCTACGGCCTCTGG
GAGGCGTTCGGGCCGGGTGCGGCCCGCGTGCCCGTCACCGCACCCAAGTCGGGCTTCGGG
CATCTGCTCGGCGCGGCCGCCCCGGCCGACGTGGCGCTCGCCCTGCGGGCCATGGCGGCG
GGCTCGGTGCCGCCCACCGTGAACCTCGACCGGCAGGCGCCGGGCATCGAGCTGGACATC
GTGCGGGGCGCGGCACGGCCGGCCCCGGAGTGGGAGCACACGCTGGTCGTGTCCCGAGGA
CTGGGAGGTGTCAACGCATGTCTGCTGCTGAGGCGTTGA
CLF2..370
CLF4..1110

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[2-245] 7.00000000000005e-45 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[254-370] 6.50000000000001e-23 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[2-259] 2.70000000000002e-55 G3DSA:3.40.47.10 [263-412] 1.09999999999999e-35 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-251] 3.10001307370206e-59 SSF53901 [213-412] 7.30000590915205e-48 SSF53901
SSF53901   Thiolase-like
SignalP No significant hit
TMHMM No significant hit
Resis_00060 Resis_00060   Resis_00080 Resis_00080
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