Resis_00140 : CDS information

Location

Organism	Streptomyces resistomycificus
Strain	HKI-237
Entry name	Resistomycin
Contig	AJ585192
Start / Stop / Direction	10,410 / 11,687 / + [in whole cluster] 10,410 / 11,687 / + [in contig]
Location	10410..11687 [in whole cluster] 10410..11687 [in contig]
Type	CDS
Length	1,278 bp (425 aa)

Annotation

Category	5.2 function unknown
Product	polyketide synthase acyl transferase domain protein
Product (GenBank)	RemE protein
Gene
Gene (GenBank)	remE
EC number
Keyword
Note	Similar to modular PKS AT domain This ORF is not involved in resistomycin biosynthesis.
Note (GenBank)
Reference	ACC Q70DW4 PmId [14982421] A gene cluster encoding resistomycin biosynthesis in Streptomyces resistomycificus; exploring polyketide cyclization beyond linear and angucyclic patterns. (J Am Chem Soc. , 2004) [18533655] Orchestration of discoid polyketide cyclization in the resistomycin pathway. (J Am Chem Soc. , 2008) comment [PMID: 14982421](2004) resistomycin生合成gene cluster全体を異種性発現により同定。 RemE: MCAT (malonyl-CoA:ACP transacylase) MCATsはtype II PKS gene clusterによってたいていコードされない。holo-ACPはself-malonylationができる。 type II PKSs, FASsのMCATsよりもmodular PKSsのAT domainと近しく関連がある。 --- [PMID:18533655](2008) RemEはmodular type I PKS由来のAT domainsに似たタンパクで、polyketide生合成のために必要ではない。(unpublished obsetrvations) minimal rem PKSを検証。 remABDCI + remFGLでresistomycin産生可能。
Family/Domain	FD IPR014043, IPR020801 comment [IPR014043] Acyl transferase (Domain) [IPR020801] Polyketide synthase, acyl transferase domain (Domain)

Sequence

>polyketide synthase acyl transferase domain protein [RemE protein]
MAYDARTTHLLTLSADTSHALDEVARDTAARLARLGGASPAAWCAEAAGGPHSGQRMGVV
GRTAGEMADRIGAHLAGEPVREVSVGHADPARRPPVAFLFSGQGAQYAGMGRGLHRTEPV
FRAAFDRCAEAARPFTAVPLQDLLDPGAGPAVVHRLAHAALGTFCVNYALAELWRSWGVE
PAAVLGFSSGEYAATCWAGVLDPEDAVLLLGTQVTLAERVTDGAMAVVGLGEEAALELLA
ETGPSVGLAAVISPGEVSLSGRKAELARLVDRLTASGVRTSALPVSSGLHSPLQEPAADE
LRAVAAGIRTRPPRIPLVSTVTGAGVKGPPAPEHWVRHLSGPVRFLNAVRVLDELGVRAH
VEAGPGRALVGLGARCLPGEGRLWLASLGRSTDGTGPMLMALGRLHTAGVPVGWSGVYPG
SRSAV

>polyketide synthase acyl transferase domain protein [RemE protein]
ATGGCGTACGACGCCCGCACCACCCACCTCCTCACCCTCTCCGCCGACACCTCTCACGCC
CTCGACGAAGTCGCCCGCGACACCGCCGCCCGGCTCGCCCGGCTCGGCGGGGCCTCACCG
GCCGCGTGGTGCGCCGAGGCCGCCGGAGGGCCGCATTCCGGCCAACGGATGGGCGTGGTG
GGCCGCACGGCGGGTGAGATGGCCGACCGGATCGGCGCCCACCTGGCGGGCGAGCCGGTC
CGGGAGGTGTCCGTCGGGCACGCGGACCCGGCTCGCCGGCCGCCGGTCGCCTTCCTGTTC
AGCGGTCAGGGGGCGCAGTACGCCGGGATGGGCCGGGGTCTGCACCGCACCGAACCCGTC
TTCCGGGCCGCCTTCGACCGGTGCGCGGAGGCGGCCCGGCCGTTCACCGCCGTTCCCCTG
CAGGACCTGCTCGACCCCGGCGCGGGGCCGGCCGTGGTGCACCGCCTCGCCCACGCGGCC
CTCGGCACGTTCTGCGTGAACTACGCGCTGGCCGAGCTGTGGCGCAGCTGGGGGGTGGAA
CCCGCGGCGGTGCTCGGCTTCAGTTCGGGCGAGTACGCGGCCACCTGCTGGGCCGGGGTC
CTCGACCCGGAGGACGCGGTGCTGCTGCTGGGCACGCAGGTCACCCTGGCCGAGCGGGTC
ACGGACGGCGCGATGGCGGTGGTCGGCCTGGGGGAGGAGGCCGCGCTGGAACTCCTCGCG
GAGACCGGGCCGTCGGTCGGGCTCGCGGCGGTCATCTCCCCGGGCGAGGTGTCCCTGTCG
GGCCGGAAAGCGGAGCTCGCCCGACTGGTCGACCGCCTCACGGCCTCCGGTGTCCGTACC
AGCGCTCTGCCGGTGTCGTCGGGTCTGCACTCCCCGCTCCAGGAACCGGCGGCGGACGAA
CTGCGGGCCGTGGCCGCGGGGATCCGCACGCGTCCGCCCCGCATCCCCCTCGTGTCCACG
GTCACCGGCGCCGGGGTCAAGGGCCCGCCTGCCCCCGAGCACTGGGTCCGGCACCTGAGC
GGCCCGGTGCGGTTCCTAAACGCCGTCCGCGTCCTGGACGAACTCGGTGTCCGCGCCCAT
GTGGAGGCCGGGCCCGGCCGGGCGCTGGTCGGGCTCGGGGCCCGCTGTCTGCCGGGGGAG
GGGCGGCTGTGGCTGGCCTCGCTCGGCCGGAGCACGGACGGCACCGGGCCCATGCTCATG
GCGCTGGGGCGGTTGCACACGGCGGGTGTTCCGGTGGGCTGGAGCGGGGTTTACCCAGGG
TCCCGTTCCGCCGTATAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT
SignalP	Eukaryota Bacteria, Gram-negative
TMHMM	No significant hit

Resis_00130 Resis_00150