Aran_00140 : CDS information

Location

Organism	Streptomyces echinatus
Strain	Tü303
Entry name	Aranciamycin
Contig	DQ915964
Start / Stop / Direction	19,957 / 18,737 / - [in whole cluster] 19,957 / 18,737 / - [in contig]
Location	complement(18737..19957) [in whole cluster] complement(18737..19957) [in contig]
Type	CDS
Length	1,221 bp (406 aa)

Annotation

Category	5.1 general function
Product	putative two-component system histidine kinase
Product (GenBank)	signal transduction histidine kinase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	orf14
Reference	ACC A1C182 PmId [17357167] Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. (Chembiochem. , 2007) comment aranciamycin生合成gene cluster(ara-cluster)のクローニングとシークエンス報告。 ORF14(406aa): kinase 配列解析。 ORF14はシグナル伝達histidine kinaseをコードするかもしれない。
Related Reference	ACC O34757 PmId [15090506] The membrane fluidity sensor DesK of Bacillus subtilis controls the signal decay of its cognate response regulator. (J Bacteriol. , 2004) comment Blast 137th, id31%, 2e-18, N末alignment悪い(TMSsあり) B.subtilis_desK Sensor histidine kinase desK(EC 2.7.13.3) DesKのC末がautokinaseで、DesRにリン酸パスを行なっていることを示している。また、DesKのN末にある膜貫通領域がsenseに必須であるとも言っている。
Family/Domain	FD IPR011712 comment [IPR011712] Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain (Domain) function; two-component sensor activity, protein dimerization activity

Sequence

>putative two-component system histidine kinase [signal transduction histidine kinase]
MRGLRRGALVPWWPVLIVVLLQCGLALAGLSAMLGTGATATAVVAWAVLLPPLVVLQVHH
TLHGAVLARRRRRWAGVVAQAALTYLPVLVWGVEWPAMAGFAAGSAQAVLNGPAGNVSFA
VIVAVAWPVAVGSGLPQYQAATVTAVTAAVGLGVAALMRMAWRLCRLDHARDTAVRDAAH
QERLRIARDLHDLVASRLTMAALRGELAERYLTGQDERARAELRTAVELARDALTDVRSL
AHGIQTLSLSGELNVARATLRAVGVDVTIRREPCRLAAEVENVLAAALREAVTNLLRHST
AGRCRITVRRRNGQVVLCVANDGVPEELRVPARERWELPRERRGLGNLSGRASAVGGSCT
TAVTPDGWFLLTVACPVLRTDPQLRAASPRPGLSGPRRDDCAPRAS

>putative two-component system histidine kinase [signal transduction histidine kinase]
GTGCGGGGCTTGCGTAGGGGAGCCTTGGTTCCGTGGTGGCCGGTACTGATCGTCGTGCTC
CTGCAGTGCGGGCTGGCCCTTGCCGGACTTTCGGCCATGCTCGGCACCGGGGCCACGGCC
ACCGCGGTGGTGGCCTGGGCGGTGTTGCTGCCCCCCTTGGTGGTCCTTCAGGTCCATCAC
ACGCTGCACGGCGCGGTCCTGGCGAGACGGCGCCGCCGGTGGGCCGGCGTGGTGGCGCAG
GCGGCTCTCACCTATCTGCCGGTGCTGGTCTGGGGAGTGGAGTGGCCGGCCATGGCCGGG
TTCGCCGCCGGGTCGGCGCAGGCGGTGCTCAACGGCCCGGCCGGGAACGTGTCGTTCGCG
GTCATCGTGGCGGTCGCGTGGCCGGTCGCCGTGGGCAGCGGGCTGCCGCAGTACCAGGCG
GCGACCGTCACGGCGGTGACCGCCGCGGTGGGTCTGGGGGTGGCGGCCCTGATGCGGATG
GCCTGGCGGCTGTGCCGGCTGGACCACGCCCGGGACACCGCGGTGCGCGACGCCGCCCAT
CAGGAGCGGCTGCGGATCGCCCGGGACCTGCACGATCTGGTGGCGAGCCGGCTCACCATG
GCCGCCCTCCGCGGTGAGCTGGCCGAACGCTATCTGACGGGGCAGGACGAGCGGGCCCGC
GCCGAGCTGCGCACGGCGGTCGAACTCGCCCGAGACGCGCTGACCGATGTGCGGTCGCTG
GCCCACGGGATCCAGACGCTGTCGCTGTCCGGGGAACTGAACGTGGCCCGCGCCACCTTG
CGGGCGGTGGGCGTGGACGTGACCATCCGCAGGGAGCCCTGCCGGCTGGCGGCCGAGGTC
GAGAACGTGCTCGCGGCGGCCCTGCGGGAGGCGGTGACCAACCTGCTGCGGCACAGCACG
GCCGGCCGGTGCCGTATTACGGTGCGCCGCAGGAACGGCCAGGTCGTGCTGTGCGTGGCC
AACGACGGCGTGCCCGAGGAGCTGCGGGTGCCGGCGCGGGAGCGGTGGGAGCTGCCGCGG
GAGCGGCGCGGGCTGGGCAATCTGTCCGGCCGGGCGAGCGCCGTCGGCGGGTCGTGCACG
ACGGCCGTGACCCCCGACGGCTGGTTCCTGCTCACCGTGGCCTGCCCCGTGCTCCGCACG
GATCCGCAGCTCAGGGCAGCCAGCCCGCGTCCTGGGCTATCCGGACCGCGTCGAGACGAC
TGCGCGCCCCGAGCTTCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003594 ATPase-like, ATP-binding domain (Domain) SM00387 HATPase_c SSF55874 ATP_bd_ATPase G3DSA:3.30.565.10 ATP_bd_ATPase IPR011712 Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain (Domain) PF07730 HisKA_3
SignalP	Eukaryota Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	Transmembrane 5

Aran_00130 Aran_00150