Fred_00050 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 49344
Entry name	Fredericamycin
Contig	AF525490
Start / Stop / Direction	5,782 / 7,263 / + [in whole cluster] 5,782 / 7,263 / + [in contig]
Location	5782..7263 [in whole cluster] 5782..7263 [in contig]
Type	CDS
Length	1,482 bp (493 aa)

Annotation

Category	4.3 transport
Product	putative peptide transporter
Product (GenBank)	putative peptide transporter
Gene	fdmT
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	FdmT
Reference	ACC Q2MGD4 PmId [16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005) comment Fredericamycin(FDM) gene clusterの同定論文。 fdmT: peptide transporter S.collinusのputative peptide transporter_RUB-ORF3に明らかな配列類似あり(id33%)。代謝産物を排出してfredericamycin A抵抗性を与えると考えられている。 fdmC-fdmT3(fdmTを含む)のfragmentをS.albusへ導入し、FDM-A産生に十分であることを確認している。
Related Reference	ACC P0C2U3 PmId [8157671] ( , ) comment Blast 319th, id32%, 2e-60 Lactococcus lactis subsp. cremoris_dtpT Di-/tripeptide transporter dipeptide輸送能欠損Pro要求性E.coliを用いた補完実験で、dtpTを同定。

Sequence

>putative peptide transporter [putative peptide transporter]
MTLDEGTSADSVTRTDPERPLRSMFRERWFSTLFMTDMWERFSFYGMQALLFLYATAPVA
EDGLGLTAGTAGALFGLYVSGSFLAAMPGGWLGDRVLGTHRAMLCGALVIMAGHLSMAVP
AQATFYLGLVLVACGTGLLKPNLPVMFDQMNPGASSSRRQAAFSLFYMSIQVSALVGPLV
TGILGERVNWHLGFGAAAVGMAFGVLQYLHGAPSLRGAATGPSRPLPPAALRRLVRRCWA
ALAGAVLLGAGLALSGGLPLRPVLALCGLASLVAPVWYFRRLLRDRSLGEAERARVRGYV
RVFVPSVLFWAMFGQVGSVFSLFAREHTDREVAGFSVPASWFQSAHPLFLLLLAPVFAWL
WLRIGARAGVARKFSVGLLCAGGGFAFLAVGAWLASGGRAVVSPLWLLAAFLCMTCGELV
FGPVGLSTTAESTPAGHGSRMMGLFYLGAALGAGVGGQLSHLVGVVPLWSYLAGFVVAAV
ATALLLPRRTAEI

>putative peptide transporter [putative peptide transporter]
ATGACTCTCGACGAGGGGACGTCCGCGGACTCCGTCACCCGGACCGACCCGGAGCGGCCG
CTCCGGTCGATGTTCCGTGAGCGCTGGTTCTCCACCCTCTTCATGACCGACATGTGGGAG
CGGTTCAGCTTCTACGGCATGCAGGCGCTGCTCTTCCTCTACGCCACGGCGCCGGTCGCC
GAGGACGGACTGGGCCTGACCGCCGGTACCGCGGGAGCCCTGTTCGGCCTCTACGTGTCG
GGTTCGTTCCTGGCGGCCATGCCGGGCGGCTGGCTGGGCGACCGGGTGCTGGGCACGCAC
CGGGCCATGCTCTGCGGTGCCCTGGTCATCATGGCCGGGCACCTGTCCATGGCCGTTCCG
GCCCAGGCCACCTTCTACCTCGGCCTGGTCCTGGTGGCCTGCGGCACCGGGCTGCTGAAG
CCCAATCTGCCGGTCATGTTCGACCAGATGAACCCCGGCGCGTCCAGCAGCCGGCGGCAG
GCGGCGTTCTCGCTCTTCTACATGAGTATCCAGGTGAGCGCCCTGGTGGGGCCGCTGGTC
ACGGGGATCCTCGGGGAACGGGTCAACTGGCACCTGGGGTTCGGCGCGGCCGCCGTGGGC
ATGGCCTTCGGGGTGCTCCAGTACCTGCACGGGGCACCGTCGCTGCGCGGAGCGGCGACG
GGCCCCAGCAGGCCCCTGCCGCCCGCCGCTCTGCGCCGCCTGGTCCGCCGGTGCTGGGCG
GCCCTGGCCGGCGCCGTGCTGCTGGGGGCGGGACTGGCACTCTCGGGGGGTCTGCCGCTG
CGTCCGGTCCTGGCCTTGTGCGGGCTGGCCTCCCTGGTGGCGCCCGTCTGGTACTTCCGG
CGGCTGCTGCGGGACCGGTCGCTGGGCGAGGCCGAACGGGCGCGGGTGCGCGGCTACGTG
CGCGTCTTCGTGCCGTCGGTACTGTTCTGGGCGATGTTCGGGCAGGTGGGCTCGGTCTTC
AGCCTGTTCGCCCGGGAGCACACCGATCGCGAGGTGGCCGGATTCAGCGTGCCGGCGAGC
TGGTTCCAGTCGGCGCATCCGCTCTTCCTGCTGTTGTTGGCACCGGTGTTCGCCTGGCTC
TGGCTGAGGATCGGGGCGCGTGCGGGGGTGGCGCGGAAATTCTCCGTGGGGCTGCTCTGC
GCGGGCGGCGGCTTCGCCTTCCTCGCCGTCGGCGCCTGGCTCGCCTCCGGCGGGCGGGCG
GTGGTGTCCCCGTTGTGGCTGCTGGCCGCCTTCCTCTGCATGACCTGCGGTGAGCTGGTC
TTCGGGCCGGTCGGACTGAGTACCACCGCCGAGTCGACCCCCGCAGGCCACGGCAGCCGG
ATGATGGGGCTCTTCTACCTCGGGGCGGCGCTGGGCGCCGGTGTCGGCGGGCAGCTGTCG
CACCTGGTGGGTGTCGTCCCGCTCTGGAGCTACCTCGCGGGGTTCGTCGTGGCCGCAGTG
GCCACCGCGCTGCTGTTGCCCCGGCGAACGGCGGAAATCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000109 Oligopeptide transporter (Family) PF00854 PTR2 IPR005279 Amino acid/peptide transporter family (Family) TIGR00924 YjdL_sub1_fam IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain) SSF103473 MFS_gen_substrate_transporter
SignalP	Eukaryota
TMHMM	Transmembrane 14

Fred_00040 Fred_00060