Dauno_00200 Dauno_00200   Dauno_00020 Dauno_00020

Dauno_00010 : CDS information

close this sectionLocation

Organism
StrainC5
Entry nameDaunorubicin
Contig
Start / Stop / Direction4,087 / 3,224 / - [in whole cluster]
891 / 28 / - [in contig]
Locationcomplement(3224..4087) [in whole cluster]
complement(28..891) [in contig]
TypeCDS
Length864 bp (287 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)ketoreductase
GenedauU
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • orf1
Reference
ACC
PmId
[8655529] Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5. (J Bacteriol. , 1996)
comment
Daunorubicin (DNR) 生合成PKSの上流領域にある6ORFsの同定。

orf1: Ketoreductase
similar to Streptomyces antibioticus Orf4 (30%)

Orf1はketoreductase候補だが、NADPH存在下でaklaviketone → aklavinoneの還元を2.9%しかできない。promoter下にorf1またはdauBを置いて過剰発現したplasmidでは、dauE(aklaviketone reductase) mutant strainを相補できない。

DNR生合成で知られたketoreductase-like functionで唯一残っているのはC-13 keto基の還元であるが、orf1がこの機能をコードするかどうかは今のところ分かっていない。→続報も出ていない
Related Reference
ACC
Q9ZAU1
NITE
Adria_00030
PmId
[9864344] Doxorubicin overproduction in Streptomyces peucetius: cloning and characterization of the dnrU ketoreductase and dnrV genes and the doxA cytochrome P-450 hydroxylase gene. (J Bacteriol. , 1999)
comment
Blast 2nd, id95%, 1e-145
Streptomyces peucetius_dnrU
Daunorubicin C-13 ketoreductase
[DoBISCUIT]putative oxidoreductase

dnrU破壊株の代謝産物は、wildと比べて、doxorubicin (DXR)が約3.4倍、RHOが約5.8倍に増える。
dnrX mutantではDXR産生が3倍に、RHOは検出されず。

dnrX dnrU double mutantはDXR産生で加算的な効果がある。
dnrX dnrU dnrH triple mutantは、dnrX dnrU double mutantより3.4-3.8倍多いDXRを産生。13-Deoxy-DNRも13-dihydro-DNRも産生されない。

これらのデータから完全な説明はできないが、DnrUはdaunorubicin (DNR) or DXR → anthracycline系代謝のその他の産物への経路が考えられている。
DNR → (13S)-13-dihydro-DNR ？
Family/Domain
FD
IPR002198
comment
[IPR002198] Short-chain dehydrogenase/reductase SDR (Family)
function; oxidoreductase activity

close this sectionSequence

selected fasta
>putative oxidoreductase [ketoreductase]
MTVPTPRHGTPHGGLPGRTVLITGATSGIGRAAALAIARQGARVVLVGRDPERLRTVTNE
VARTAGPAPDAFRADFAELRQVRELGERLRDRYPRIDVMAGNAGGMFWSRTTTQDGFEAT
LQVNHLAGFLLARLLRERLAGGRLILTSSDAYTQGRIDPDDLNGDRHRYSAGQAYGTSKQ
ANIMTATEAARRWPDVLTVSYHPGEVRTRIGRGTVASTYFRFNPFLRSAAKGADTLVWLA
AAPAEELTTGGYYSDRRLSPVSGPTADAGLAAKLWEASAAAVGDTAR
selected fasta
>putative oxidoreductase [ketoreductase]
ATGACGGTCCCCACCCCGCGCCACGGGACACCACACGGCGGCCTGCCGGGCCGGACGGTG
CTGATCACCGGGGCCACGTCCGGCATCGGCCGGGCGGCGGCCCTCGCGATCGCCCGCCAG
GGGGCCCGCGTCGTGCTCGTCGGCCGGGACCCCGAGCGTCTTCGGACCGTCACGAACGAG
GTGGCCCGGACCGCCGGCCCGGCCCCGGACGCCTTCCGCGCGGACTTCGCCGAGCTGCGC
CAGGTACGCGAGCTGGGAGAGCGGCTGCGGGACCGGTACCCGCGCATCGATGTCATGGCC
GGCAACGCCGGCGGCATGTTCTGGTCGCGCACCACGACCCAGGACGGGTTCGAGGCCACC
CTCCAGGTCAATCATCTCGCGGGCTTCCTGCTGGCACGGCTGCTGCGGGAGCGGCTCGCG
GGCGGGCGGTTGATCCTCACCTCGTCCGACGCGTACACCCAGGGCCGGATCGACCCGGAC
GATCTCAACGGCGACCGTCACCGCTACAGCGCGGGCCAGGCGTACGGCACGTCCAAACAG
GCCAACATCATGACCGCCACGGAGGCCGCCCGGCGCTGGCCGGACGTGCTGACGGTCAGC
TACCACCCCGGCGAGGTCCGCACCCGCATCGGGCGGGGCACAGTCGCCTCGACCTACTTC
CGGTTCAACCCCTTCCTGCGGTCCGCGGCCAAGGGCGCCGACACTCTCGTGTGGCTGGCG
GCCGCGCCGGCCGAGGAGTTGACCACGGGCGGCTACTACAGCGACCGGCGGCTGTCCCCG
GTGAGCGGCCCGACCGCCGACGCCGGCCTCGCGGCCAAGCTCTGGGAGGCCAGCGCGGCC
GCCGTCGGCGACACCGCGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[19-155] 8.49999999999995e-20 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[19-36] 7.20000348184352e-08 PR00081 [94-105] 7.20000348184352e-08 PR00081 [136-152] 7.20000348184352e-08 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[8-279] 1.70000000000001e-49 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Dauno_00200 Dauno_00200   Dauno_00020 Dauno_00020
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