Dauno_00030 Dauno_00030   Dauno_00050 Dauno_00050

Dauno_00040 : CDS information

close this sectionLocation

Organism
StrainC5
Entry nameDaunorubicin
Contig
Start / Stop / Direction5,047 / 6,597 / + [in whole cluster]
1,851 / 3,401 / + [in contig]
Location5047..6597 [in whole cluster]
1851..3401 [in contig]
TypeCDS
Length1,551 bp (516 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose 2,3-dehydratase
Product (GenBank)
GenednmT
Gene (GenBank)
EC number
Keyword
  • daunosamine
Note
Note (GenBank)
  • orf3
Reference
ACC
PmId
[8655529] Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5. (J Bacteriol. , 1996)
comment
Daunorubicin (DNR) 生合成PKSの上流領域にある6ORFsの同定。

Orf3: unknown function
Related Reference
ACC
P95833
NITE
Adria_00060
PmId
[8955419] Enhanced antibiotic production by manipulation of the Streptomyces peucetius dnrH and dnmT genes involved in doxorubicin (adriamycin) biosynthesis. (J Bacteriol. , 1996)
comment
Blast 2nd, id81%, 0.0
Streptomyces peucetius_dnmT
Doxorubicin biosynthesis protein DnmT
[DoBISCUIT]putative NDP-hexose 2,3-dehydratase

dnmT mutantsはRHOが3倍増、DNR検出されず。
dnmT(-) strainでdnmTを過剰発現すると、RHO, DNR共にwildレベルに回復。
よって、dnmTはdaunosamineの生合成かRHOへの付着に関与するか、この過程の調節に関与するかもしれない。

dnrH mutant strainsでのdnmT過剰発現は、RHOが192倍減、DNRが9.4倍増。
ACC
Q9ALN6
NITE
Spino_00070
PmId
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
Blast 27th, id46%, 5e-98
Saccharopolyspora spinosa_spnO
Probable NDP-hexose-2,3-dehydratase

dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO

dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。
dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。

--
SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

close this sectionSequence

selected fasta
>putative NDP-hexose 2,3-dehydratase
MRRHSAAATTVQIARSVLARDGLCSGMDRFWAWYAERSARVVHRTERIPLDALKGWSRHP
VTGTISHHTGRFFSIEGLDVHIPGAPVPRWSQPIVNQPEVGILGFLVKERGGILHCLVQA
KFEPGNPGGLQLSPTVQATRSNYTRVPGGKTIPSWSTSATPPDRQVVADVLQSGQGSWFY
RKRNRNMIVQVFDEVPLREDFHWLTLGQLHRLLGVENLVNMDARTVLACLPSRARERARW
PTRSPPELTAPGASAHPVVPSLGPTAAGLRWTPGFHHSLVRSCAAAEGSLHSTVDIVSWI
ATCAAGPMWSPARQANSLPHWIERDGVIATRAGRFLEVMAVDVTAASREVPGWSQPMIEP
KGRGVAAFLVRRIDGVLHALGTARVEPGYVDVVEIAPTVQCTPGSLHALPAGARPRFLDA
VLEAPPEAACATTTDLAEEGGRFHHAVNTYMIVEADDDIPDGGEFRWLTLHQLVELLRHS
HYVNVQARTLVACLHSLSVGHRPSTRSAVPSAPPRR
selected fasta
>putative NDP-hexose 2,3-dehydratase
GTGCGCCGGCACAGTGCTGCCGCGACGACCGTCCAGATCGCCCGCTCCGTCCTCGCCCGG
GACGGCCTGTGCAGCGGCATGGACCGGTTCTGGGCGTGGTACGCCGAGCGCTCGGCGCGG
GTGGTGCACCGCACCGAGCGGATTCCGCTGGATGCCCTCAAGGGGTGGAGTCGCCACCCG
GTCACCGGGACCATCTCCCATCACACCGGAAGGTTCTTCAGCATCGAGGGACTGGACGTG
CACATTCCGGGCGCCCCGGTGCCACGCTGGTCCCAGCCGATCGTGAACCAGCCCGAAGTG
GGCATCCTGGGCTTCCTGGTGAAGGAACGCGGCGGGATCCTCCACTGCCTCGTCCAGGCC
AAGTTCGAGCCGGGCAACCCCGGCGGACTTCAGCTCTCCCCCACGGTCCAGGCCACACGG
AGCAACTACACGCGGGTGCCCGGCGGCAAGACCATCCCGTCCTGGAGCACTTCCGCGACA
CCTCCGGACCGGCAGGTGGTCGCCGATGTGCTCCAGTCGGGACAGGGCTCCTGGTTCTAC
CGCAAACGCAACCGCAACATGATCGTCCAGGTGTTCGACGAGGTCCCGCTGCGCGAGGAC
TTCCACTGGCTCACGCTGGGTCAGCTGCACCGGCTGCTGGGGGTCGAGAACCTCGTGAAC
ATGGACGCCAGGACGGTGCTGGCCTGCCTGCCGTCGCGAGCGAGGGAGCGCGCCCGCTGG
CCGACGCGGAGCCCGCCGGAGCTGACGGCCCCGGGGGCGTCAGCCCACCCCGTGGTCCCC
TCGCTCGGCCCGACGGCGGCGGGTCTCCGGTGGACGCCCGGTTTCCACCACTCCCTCGTG
CGATCGTGCGCGGCGGCCGAGGGCAGCCTGCACAGCACGGTGGACATCGTCAGCTGGATC
GCGACCTGCGCAGCCGGACCGATGTGGTCACCCGCCCGACAGGCTAACTCGCTGCCGCAC
TGGATCGAGCGGGACGGCGTGATCGCCACGAGAGCCGGGCGCTTCCTGGAGGTGATGGCC
GTGGATGTGACGGCCGCCTCGCGAGAGGTGCCCGGCTGGAGTCAGCCGATGATCGAACCC
AAGGGCCGAGGGGTCGCGGCCTTCCTGGTCCGGCGGATCGACGGGGTTCTCCACGCCCTC
GGGACCGCGCGCGTGGAGCCCGGCTACGTCGACGTGGTGGAGATCGCGCCGACCGTGCAG
TGCACCCCCGGCAGTCTGCACGCCCTGCCGGCCGGAGCCAGGCCCCGCTTCCTCGACGCC
GTCCTGGAGGCGCCCCCCGAAGCAGCGTGCGCTACGACGACCGACCTCGCGGAGGAGGGA
GGACGCTTCCACCACGCGGTGAACACTTACATGATCGTCGAAGCCGACGATGACATCCCG
GACGGTGGTGAGTTCCGGTGGCTGACGCTCCACCAACTCGTCGAGCTGCTGCGTCACTCC
CACTACGTCAACGTCCAGGCCCGCACCCTGGTCGCCTGCCTGCACAGCCTGTCCGTGGGC
CACCGCCCGTCGACCCGCTCGGCCGTGCCGTCCGCCCCGCCTCGGAGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005212 NDP-hexose 2,3-dehydratase (Domain)
[28-230] 1.29999999999998e-75 PF03559 [291-494] 4.80000000000003e-69 PF03559
PF03559   Hexose_dehydrat
SignalP
[1-19] 0.934 Signal
Bacteria, Gram-negative   
[1-19] 0.827 Signal
Eukaryota   
TMHMM No significant hit
Dauno_00030 Dauno_00030   Dauno_00050 Dauno_00050
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