Dauno_00190 Dauno_00190   Dauno_00090 Dauno_00090

Dauno_00080 : CDS information

close this sectionLocation

Organism
StrainC5
Entry nameDaunorubicin
Contig
Start / Stop / Direction18,433 / 17,771 / - [in whole cluster]
1,163 / 501 / - [in contig]
Locationcomplement(17771..18433) [in whole cluster]
complement(501..1163) [in contig]
TypeCDS
Length663 bp (220 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
Productaklanonic acid O-methyltransferase
Product (GenBank)aklanonic acid methyltransferase
Gene
Gene (GenBank)dauC
EC number2.1.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7836284] Analysis of clustered genes encoding both early and late steps in daunomycin biosynthesis by Streptomyces sp. strain C5. (J Bacteriol. , 1995)
comment
Daunorubicin (DNR)のPKS genes領域に近接した4ORFsの同定。

dauCは、Streptomyces sp. strain C5のdauC-blocked mutantsを相補し、vitroでmutant strainにaklanonic acid methyltransferase活性を回復し、vitroでStreptomyces lividansにaklanonic acid methyltransferase活性を与える。
Related Reference
ACC
Q54818
NITE
Adria_00310
PmId
[7601857] Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995)
comment
Blast 2nd, id94%, 1e-110, C末余る
Streptomyces peucetius
Putative uncharacterized protein
[Adria_00310]aklanonic acid O-methyltransferase_dnrC

Streptomyces lividans TK24にpWHM918 (carrying dnrC)導入。
この株はaklanonic acid → aklanonic acid methylester(AAME)の変換ができる。
この変換でできた産物をdpsB(-)mutantに加えると、RHOへ変換可能。よって正しい中間体。

これらの演繹は、相同なStreptomyces sp. strain C5 dauC geneの機能と完全に一致する。

close this sectionSequence

selected fasta
>aklanonic acid O-methyltransferase [aklanonic acid methyltransferase]
MQDSSYKKQVTQAFDQSSSTYDRLGVEFFTPMGRRLVDISEPVTGERVLDIGCGRGACLF
PAAEKVGSQGCVHGIDIAPGMIEEARKEATERGLRNISLMVMDAETPGFPARSFDLVMGS
YSVIFLPDAVGALARYADILDHGGRIAFTSPVFRAGTFPFLPPEFTPLIPQALLEHLPEQ
WRPEALVRRFNSWLERAEDLVRTLEGCGYARLRQSTSRCG
selected fasta
>aklanonic acid O-methyltransferase [aklanonic acid methyltransferase]
GTGCAGGATTCGTCCTACAAGAAACAGGTGACTCAGGCCTTCGACCAGTCGTCGTCCACT
TATGATCGACTGGGGGTGGAATTCTTCACGCCCATGGGTCGCCGCCTGGTGGACATCTCG
GAGCCGGTCACGGGTGAACGAGTGCTCGACATCGGCTGCGGGCGCGGCGCATGCCTTTTC
CCGGCTGCGGAAAAGGTCGGCTCCCAGGGGTGCGTCCACGGTATCGACATCGCTCCCGGA
ATGATCGAGGAAGCTCGCAAGGAAGCCACCGAACGCGGTCTGCGGAATATCAGCCTCATG
GTGATGGATGCGGAGACACCCGGATTTCCGGCGCGTTCCTTCGACCTGGTCATGGGGAGC
TACAGCGTCATTTTCCTGCCGGATGCCGTGGGGGCCCTCGCGCGCTACGCCGACATCCTG
GACCACGGCGGCAGGATCGCGTTCACCAGCCCGGTCTTCCGTGCCGGCACGTTCCCGTTC
CTGCCGCCGGAGTTCACCCCGCTCATCCCCCAGGCATTGCTGGAGCACCTCCCCGAGCAG
TGGCGGCCCGAGGCGCTGGTCCGGCGGTTCAACAGCTGGCTGGAGCGAGCCGAGGATCTT
GTCCGTACCCTTGAAGGCTGCGGTTACGCTCGTCTCCGTCAGTCGACGAGCCGGTGCGGA
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Dauno_00190 Dauno_00190   Dauno_00090 Dauno_00090
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