Dauno_00060 Dauno_00060   Dauno_00140 Dauno_00140

Dauno_00130 : CDS information

close this sectionLocation

Organism
StrainC5
Entry nameDaunorubicin
Contig
Start / Stop / Direction10,501 / 9,554 / - [in whole cluster]
1,320 / 373 / - [in contig]
Locationcomplement(9554..10501) [in whole cluster]
complement(373..1320) [in contig]
TypeCDS
Length948 bp (315 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative cyclase/dehydratase
Product (GenBank)cyclase/dehydrase
GenedpsF
Gene (GenBank)dauA
EC number
Keyword
  • 1st ring
Note
Note (GenBank)
  • ORFF; putative
Reference
ACC
PmId
[7928998] Isolation and sequence analysis of polyketide synthase genes from the daunomycin-producing Streptomyces sp. strain C5. (J Bacteriol. , 1994)
[9098068] Minimal Streptomyces sp. strain C5 daunorubicin polyketide biosynthesis genes required for aklanonic acid biosynthesis. (J Bacteriol. , 1997)
comment
[PMID: 7928998](1994)
PKS genesを含む7ORFsの同定。

DauA-OrfF: Cyclase/dehydrase

actinorhodin biosynthesis bifunctional cyclase/dehydraseであるS.coelicolor ActVIIとの配列解析(id44%, dot plotもあり)に基づく。
ActVII mutantをdauA-orfF, dauB, dauA-orfEABCDの7ORFを含んだfragmentまたはdauA-orfF+dauB+dauA-orfEのfragmentで相補可能。

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[PMID: 9098068](1997)
daunorubicin (DNR), doxorubicin (DXR)の前駆体であるaklanonic acidを異種性ホストで産生するために必要な最小遺伝子は、dpsG (ACP), dauI (regulatory activator), dpsA (KS alpha), dpsB (KS beta), dpsF (aromatase), dpsE (polyketide reductase), and dauG (putative deoxyaklanonic acid oxygenase)であるということを実証している。
Related Reference
ACC
Q54811
NITE
Adria_00240
PmId
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 2nd, id94%, 1e-168
Streptomyces peucetius_dpsF
Daunorubicin-doxorubicin polyketide synthase
[DoBISCUIT]cyclase/dehydrase

minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
JadD, DpsFの両cyclasesはC-9で還元された10-ketidesの位置特異的なfirst-ring cyclase/dehydrasesであることが示された。

close this sectionSequence

selected fasta
>putative cyclase/dehydratase [cyclase/dehydrase]
MSESPLQQTEHEIHTSAAPDAVFAVLADVRSWPAVFPPSVHVEQLECADSSERIRIWATA
NGSLRTWTSRRELDERVRRIRFRQETSAHPVAAMGGEWIVEEAGDGGTRVRLTHDFRAVD
DDPETIGWIHRAVDRNSEAELASLRTALEGPDGTAPTTFEDTVVVRGSAEDIYRFLHRSD
LWEKRLSHVARIAVKEEEPGLQHMEMDTLTADGSVHTTASVRVCFPERRLIVYKQLRTPP
LLALHLGRWSVRPAGDGDGIAVTSAHTVSVARSAIPGILGAGANETDAVDFVRRALGRNS
LLTLEAARRYAESSA
selected fasta
>putative cyclase/dehydratase [cyclase/dehydrase]
ATGTCCGAGTCGCCGCTTCAGCAGACCGAGCACGAGATCCATACCTCGGCCGCGCCGGAC
GCCGTGTTCGCCGTGCTGGCCGACGTGCGGTCCTGGCCCGCCGTGTTCCCGCCCAGTGTC
CACGTCGAACAGTTGGAGTGCGCGGACTCCAGCGAACGCATCCGCATCTGGGCCACTGCC
AACGGCTCGCTTCGTACCTGGACGTCGCGGCGCGAGCTCGACGAGCGGGTCCGGCGCATC
CGTTTCCGGCAGGAGACGTCCGCCCACCCCGTCGCAGCCATGGGCGGCGAATGGATCGTC
GAGGAAGCCGGTGACGGCGGCACCCGCGTGCGGCTGACGCACGACTTCCGGGCCGTGGAC
GACGACCCGGAGACGATCGGCTGGATCCATCGGGCCGTGGACCGCAACAGCGAAGCCGAA
CTGGCGTCGCTGCGCACCGCGCTGGAAGGACCCGACGGCACCGCTCCGACGACCTTCGAG
GACACCGTCGTCGTCCGTGGCAGTGCCGAGGACATCTACCGCTTCCTCCATCGCAGCGAC
CTCTGGGAGAAGCGGCTCTCCCACGTCGCGCGGATCGCGGTCAAGGAGGAGGAACCGGGT
CTGCAGCACATGGAGATGGACACCCTCACCGCGGACGGCTCGGTACACACCACCGCGTCG
GTGCGCGTGTGCTTCCCCGAACGCCGCCTCATCGTCTACAAGCAACTGCGCACGCCGCCG
CTGCTGGCGCTCCATCTCGGCCGCTGGTCGGTCCGTCCCGCCGGCGACGGGGACGGGATC
GCCGTCACGTCGGCCCACACCGTGTCCGTGGCCCGCTCGGCGATCCCCGGCATCCTAGGT
GCGGGGGCGAATGAGACGGACGCCGTGGACTTCGTCCGCCGGGCCCTGGGCCGCAACAGT
CTGCTGACCCTGGAAGCGGCCAGACGGTACGCCGAGTCCAGCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR019587 Polyketide cyclase/dehydrase (Family)
[8-149] 2.3e-15 PF10604 [158-312] 2.5e-06 PF10604
PF10604   Polyketide_cyc2
IPR023393 START-like domain (Domain)
[7-148] 2.3e-13 G3DSA:3.30.530.20
G3DSA:3.30.530.20   G3DSA:3.30.530.20
SignalP No significant hit
TMHMM No significant hit
Dauno_00060 Dauno_00060   Dauno_00140 Dauno_00140
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