Dauno_00130 Dauno_00130   Dauno_00150 Dauno_00150

Dauno_00140 : CDS information

close this sectionLocation

Organism
StrainC5
Entry nameDaunorubicin
Contig
Start / Stop / Direction11,319 / 10,531 / - [in whole cluster]
2,138 / 1,350 / - [in contig]
Locationcomplement(10531..11319) [in whole cluster]
complement(1350..2138) [in contig]
TypeCDS
Length789 bp (262 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative polyketide C-9 ketoreductase
Product (GenBank)polyketide reductase
GenedpsE
Gene (GenBank)dauB
EC number
Keyword
Note
Note (GenBank)
  • putative
Reference
ACC
PmId
[7928998] Isolation and sequence analysis of polyketide synthase genes from the daunomycin-producing Streptomyces sp. strain C5. (J Bacteriol. , 1994)
[9098068] Minimal Streptomyces sp. strain C5 daunorubicin polyketide biosynthesis genes required for aklanonic acid biosynthesis. (J Bacteriol. , 1997)
comment
[PMID: 7928998](1994)
PKS genesを含む7ORFsの同定。

DauB: polyketide reductase

dauA-orfF, dauB, dauA-orfEABCDの7ORFを含んだfragmentまたはdauA-orfF+dauB+dauA-orfEのfragmentで、polyketide reductase活性を欠損したS. galilaeus ATCC 31671の抗生物質産生を回復。
また同じfragmentで、S.coelicolor_actIII mutantも相補できている。

---
[PMID: 9098068](1997)
daunorubicin (DNR), doxorubicin (DXR)の前駆体であるaklanonic acidを異種性ホストで産生するために必要な最小遺伝子は、dpsG (ACP), dauI (regulatory activator), dpsA (KS alpha), dpsB (KS beta), dpsF (aromatase), dpsE (polyketide reductase), and dauG (putative deoxyaklanonic acid oxygenase)であるということを実証している。
Related Reference
ACC
Q54812
NITE
Adria_00250
PmId
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 2nd, id90%, 1e-130
Streptomyces peucetius_dpsE
Daunorubicin-doxorubicin polyketide synthase
[DoBISCUIT]9-ketoreductase

DpsEは、DpsFによる環化に先立って必要なC-9還元を担う、decaketideに特異的なketoreductaseであることが実験で確認されている。

minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。KRとして、dpsE, jadE, actIII を使用している。

DpsEはdecaketidesを還元できるが、octaketidesを還元できない。
JadEはdecaketideだけでなく、octaketideも還元できる。

JadD, DpsFの両cyclasesはC-9で還元されたdecaketidesの位置特異的なfirst-ring cyclase/dehydrasesである。

close this sectionSequence

selected fasta
>putative polyketide C-9 ketoreductase [polyketide reductase]
MSDAADRVALVTGGTSGIGLAVGRRKLALDGTRVFLCARDESAVTGTVKELQASGLEVEG
TSCDVRSTDAVDRLVRTARNPLRAIDIVVNNAGRGGGGVTAQITDDLWSDVVDTNLGGAF
RVTRAVLTGGGMQEHGWGRIINIASTGGKQGVALGAPYSASKSGLIGFTKAVALELARTG
ITVNAVCPGYVETPMAQGVRQRYAAFWGITEDDVLEKFRAKIPLGRYSTSDEVAGMVHYL
VSDSADSITAQAINVCGGLGSY
selected fasta
>putative polyketide C-9 ketoreductase [polyketide reductase]
ATGTCCGACGCAGCCGACCGGGTCGCACTTGTCACCGGGGGAACCAGCGGCATCGGTCTC
GCGGTAGGACGACGCAAGCTCGCCCTGGACGGGACCCGGGTGTTCCTGTGCGCTCGTGAC
GAGAGCGCCGTCACCGGCACCGTGAAGGAACTTCAGGCATCGGGACTGGAGGTGGAGGGC
ACGTCCTGCGACGTGCGGAGCACCGACGCTGTCGACCGGCTGGTCCGAACGGCCCGGAAC
CCGCTTCGGGCGATCGACATCGTCGTCAACAACGCCGGCCGCGGTGGTGGAGGAGTGACC
GCCCAGATCACGGACGACCTGTGGTCCGATGTCGTCGACACCAACCTCGGCGGGGCCTTC
CGGGTCACCCGCGCCGTCCTCACCGGCGGCGGCATGCAGGAGCACGGCTGGGGCCGGATC
ATCAACATCGCCTCTACCGGCGGCAAGCAGGGAGTGGCCCTCGGCGCTCCCTACTCCGCC
TCCAAGAGCGGCCTGATCGGCTTCACGAAGGCTGTCGCACTGGAGCTGGCCAGGACGGGC
ATCACGGTCAACGCCGTCTGCCCGGGGTACGTCGAGACGCCCATGGCCCAAGGCGTGCGG
CAGCGATACGCCGCCTTCTGGGGGATCACCGAGGACGACGTCCTGGAGAAGTTCCGGGCG
AAGATCCCGCTCGGACGCTACTCGACGTCCGACGAGGTGGCCGGAATGGTGCACTACCTG
GTCTCCGACAGCGCCGACTCGATCACGGCCCAGGCCATCAATGTCTGTGGTGGCCTCGGC
AGTTACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[83-94] 1.20000117458134e-13 PR00080 [138-146] 1.20000117458134e-13 PR00080 [158-177] 1.20000117458134e-13 PR00080
PR00080   SDRFAMILY
[8-176] 7.10000000000008e-35 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[8-25] 8.39998014024981e-45 PR00081 [83-94] 8.39998014024981e-45 PR00081 [132-148] 8.39998014024981e-45 PR00081 [158-177] 8.39998014024981e-45 PR00081 [179-196] 8.39998014024981e-45 PR00081 [223-243] 8.39998014024981e-45 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[3-259] 2.20000000000002e-75 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[145-173] PS00061
PS00061   ADH_SHORT
SignalP No significant hit
TMHMM No significant hit
Dauno_00130 Dauno_00130   Dauno_00150 Dauno_00150
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