Dauno_00150 Dauno_00150   Dauno_00170 Dauno_00170

Dauno_00160 : CDS information

close this sectionLocation

Organism
StrainC5
Entry nameDaunorubicin
Contig
Start / Stop / Direction11,890 / 13,146 / + [in whole cluster]
2,709 / 3,965 / + [in contig]
Location11890..13146 [in whole cluster]
2709..3965 [in contig]
TypeCDS
Length1,257 bp (418 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)polyketide synthase
GenedpsA
Gene (GenBank)
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • ORFA; putative
Reference
ACC
PmId
[7928998] Isolation and sequence analysis of polyketide synthase genes from the daunomycin-producing Streptomyces sp. strain C5. (J Bacteriol. , 1994)
[9098068] Minimal Streptomyces sp. strain C5 daunorubicin polyketide biosynthesis genes required for aklanonic acid biosynthesis. (J Bacteriol. , 1997)
comment
[PMID: 7928998](1994)
PKS genesを含む7ORFsの同定。

DauA-OrfA: PKS

機能予測はS.glaucescens TcmKとの相同性に基づく(id65%, dot plotあり)。
actI mutantの相補実験もあるが、このORF単独の機能特定には至らない。
actIはPKS subunits 6ORFsのうちどれか、または全部。ACPなしfragmentでは相補できていない。

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[PMID: 9098068](1997)
daunorubicin (DNR), doxorubicin (DXR)の前駆体であるaklanonic acidを異種性ホストで産生するために必要な最小遺伝子は、dpsG (ACP), dauI (regulatory activator), dpsA (KS alpha), dpsB (KS beta), dpsF (aromatase), dpsE (polyketide reductase), and dauG (putative deoxyaklanonic acid oxygenase)であるということを実証している。

dpsA(KS alpha)がこのentryに相当。

close this sectionPKS/NRPS Module

KS3..374

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [polyketide synthase]
MNRRVVITGMGVVAPGAIGIKSFWELLLSGTTATRAITTFDATPFRSRIAAECDFDPVAA
GLSAEQARRLDRAGQFALVAGQEALTDSGLRIGEDSAHRVGVCVGTAVGCTQKLESEYVA
LSAGGANWVVDPHRGAPELYDYFVPSSLAAEVAWLAGAEGPVNIVSAGCTSGIDSIGYAC
ELIREGTVDVMLAGGVDAPIAPITVACFDAIRVTSDHNDTPETLAPFSRSRNGFVLGEGG
AIVVLEEAEAAVRRGARIYAEIGGYASRGNAYHMTGLRADGAEMAAAITAALDEARRDPS
DVDYVNAHGTATRQNDRHETSAFKRSLGDHAYRVPISSVKSMIGHSLGAAGSLEVAATAL
AVEYGAIPPTANLHDPDPELDLDYVPLTAREKRVRHALTVGSGFGGFQSAMLLSRPER
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [polyketide synthase]
GTGAACCGACGGGTCGTCATCACCGGGATGGGTGTGGTGGCCCCGGGAGCCATCGGCATC
AAGTCGTTCTGGGAGCTGCTGCTGTCCGGGACCACCGCCACCCGGGCCATCACCACGTTC
GACGCCACGCCCTTCCGTTCCCGGATCGCCGCCGAGTGCGACTTCGATCCGGTCGCGGCA
GGGCTGTCCGCGGAGCAGGCCCGGCGCCTCGACCGGGCCGGGCAGTTCGCGCTGGTCGCC
GGTCAGGAGGCGCTCACTGACAGCGGGCTGCGCATCGGGGAGGACTCCGCCCACCGGGTC
GGGGTGTGCGTGGGTACCGCTGTCGGCTGCACGCAGAAACTGGAGTCCGAGTACGTGGCT
CTCAGCGCGGGCGGTGCGAACTGGGTCGTGGACCCCCACCGGGGGGCGCCCGAGCTGTAC
GACTACTTCGTGCCGAGTTCCCTCGCCGCCGAAGTGGCTTGGCTCGCCGGGGCGGAAGGG
CCCGTGAACATCGTCTCGGCCGGGTGCACCTCGGGGATCGACTCCATCGGGTACGCCTGC
GAACTCATCCGCGAGGGGACGGTGGACGTCATGCTCGCGGGAGGCGTGGACGCCCCCATC
GCACCGATCACTGTGGCCTGCTTCGACGCCATCAGGGTGACCTCCGACCACAACGACACA
CCCGAGACGCTCGCGCCCTTCAGCCGCAGCCGCAACGGCTTCGTGCTGGGCGAGGGTGGT
GCGATCGTGGTTCTGGAAGAGGCAGAGGCGGCCGTGCGCAGGGGCGCACGAATCTACGCC
GAGATCGGCGGGTACGCCTCACGCGGCAACGCATACCACATGACGGGGCTGCGCGCAGAC
GGCGCCGAGATGGCTGCGGCGATTACCGCGGCCCTCGACGAAGCCCGCCGCGACCCCTCG
GACGTGGACTATGTCAACGCCCACGGAACAGCGACGCGACAGAACGACCGGCACGAAACC
TCCGCGTTCAAGCGCTCGCTGGGGGATCACGCGTACCGGGTTCCCATCAGCTCCGTGAAG
TCGATGATCGGTCACTCCCTGGGGGCCGCCGGCTCGCTCGAAGTGGCGGCGACCGCACTG
GCGGTGGAGTACGGGGCGATCCCGCCGACCGCGAATCTGCACGACCCGGATCCCGAACTC
GACCTGGACTACGTGCCGTTGACGGCGCGAGAAAAGCGGGTGCGGCATGCGCTGACGGTG
GGCAGCGGCTTCGGCGGCTTCCAGAGCGCCATGCTGTTGAGCCGTCCCGAGAGGTGA
KS3..374
KS7..1122

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[3-250] 3.60000000000002e-58 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[259-374] 1.80000000000002e-38 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[2-262] 4.6e-62 G3DSA:3.40.47.10 [269-416] 1.99999999999999e-52 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-418] 6.59996953923913e-66 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[160-176] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP
[1-19] 0.583 Signal
Eukaryota   
TMHMM No significant hit
Dauno_00150 Dauno_00150   Dauno_00170 Dauno_00170
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