Urd_00020 Urd_00020   Urd_00040 Urd_00040

Urd_00030 : CDS information

close this sectionLocation

Organism
StrainTü2717
Entry nameUrdamycin
Contig
Start / Stop / Direction3,332 / 4,612 / + [in whole cluster]
3,332 / 4,612 / + [in contig]
Location3332..4612 [in whole cluster]
3332..4612 [in contig]
TypeCDS
Length1,281 bp (426 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductL-rhodinose glycosyltransferase
Product (GenBank)glycosyl transferase
Gene
Gene (GenBank)urdGT1a
EC number
Keyword
  • C-12b OH group
Note
Note (GenBank)
  • UrdGT1a; uses TTG start codon
Reference
ACC
PmId
[10662691] Function of glycosyltransferase genes involved in urdamycin A biosynthesis. (Chem Biol. , 2000)
[11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000)
comment
UniProtで登録されている[PMID: 10658661](2000)にurdGT1aについての記載はない。

--
[PMID: 10662691](2000)
[PMID: 11094336](2000)

urdamycin clusterに含まれるglycosyltransferase候補genes=urdGT1a, urdGT1b, urdGT1cについて、mutant作成、相補実験における蓄積産物解析を行い、各機能割り当てが確定されている。

UrdGT1aは、urdamycin A生合成においてC-12bのhydroxy基にL-rhodinoseを付着するO-glycosyltransferaseである。

C-9にあるtrisaccharideでもL-rhodinoseを付着するglycosyltransferase(UrdGT1c)が作用するが、UrdGT1aはこれを代替しない。

close this sectionSequence

selected fasta
>L-rhodinose glycosyltransferase [glycosyl transferase]
MRVLFSIMPATAHLYPIVPLAWALQASGHEVRVASHPDMAATITAAGLTAVSVGRPADLG
ALMRDGASDQCLEQITEALEIGSDDLNLRNAIRYYTLAGVSLYFPTDPQRAESDRAVDDL
VAFARSWRPDLVLWDPLVLAAPVAARLSGAAHARVLYGLDYVGWAQQRFTERIGRRAQPL
TEHPLAELMRPTLRRFGLDFDAELAVGQWTLDLLPERMRLPVDLRYTALRRVPYTGTTTV
PQWLYDEPKRPRVCLTLGMSTRKFLSGHGASPIADIFGLVEELDIELVATVNSAQLADVE
ELPDRVRIVDYLPLDLLLPTCSAVVHHGGGGTFAAAVAHQVPQLIPAPGEGGDRVAFARY
VEQRGAGLTVSRTDYSADVFAEQLFRVLHEPSFQDGAAGLHDDMLATPSPRDVVPVLEKL
SDRYRR
selected fasta
>L-rhodinose glycosyltransferase [glycosyl transferase]
TTGCGTGTCCTCTTTTCGATCATGCCGGCAACAGCGCACTTATATCCGATAGTTCCACTG
GCCTGGGCGCTTCAAGCTAGTGGCCACGAGGTCCGGGTCGCCAGCCACCCCGACATGGCC
GCCACCATAACCGCGGCCGGTCTGACCGCTGTCTCCGTGGGGCGGCCTGCGGACCTCGGG
GCCCTCATGCGGGACGGCGCGAGCGACCAGTGTTTGGAGCAGATCACCGAGGCCCTGGAA
ATTGGTTCCGACGATCTCAATCTGCGCAATGCCATCCGCTATTACACGTTGGCAGGTGTC
TCGCTCTATTTCCCCACCGATCCGCAGCGGGCCGAGAGCGACCGTGCGGTGGACGACCTG
GTGGCCTTCGCCCGGTCCTGGCGACCCGATCTTGTGCTGTGGGATCCCCTGGTCCTGGCG
GCTCCCGTCGCGGCCCGACTCAGCGGAGCCGCGCATGCCCGTGTCCTGTACGGGCTCGAC
TACGTCGGCTGGGCGCAGCAACGGTTCACGGAGCGGATCGGCCGACGGGCCCAGCCGCTC
ACGGAGCATCCGCTCGCGGAACTCATGCGGCCGACCCTCCGACGCTTCGGGCTCGACTTC
GACGCCGAGCTGGCCGTCGGCCAGTGGACACTCGATCTGCTGCCCGAACGCATGCGGTTG
CCGGTCGACCTGCGCTACACGGCGCTGCGCCGAGTCCCGTACACGGGCACGACCACTGTT
CCGCAATGGTTGTACGACGAGCCGAAGCGCCCGCGAGTGTGCCTCACACTGGGCATGAGT
ACACGCAAGTTTCTTTCCGGGCACGGTGCTTCGCCGATCGCCGACATCTTCGGTCTGGTG
GAGGAGCTCGACATCGAGCTGGTCGCCACCGTGAACTCCGCCCAGCTCGCTGACGTGGAG
GAACTCCCCGACCGGGTCCGCATAGTGGACTATCTCCCGCTCGATCTCCTGCTGCCGACC
TGCTCCGCGGTTGTGCACCATGGCGGCGGAGGGACCTTTGCGGCAGCGGTCGCCCACCAG
GTGCCCCAGCTGATCCCGGCACCCGGGGAAGGCGGGGACCGGGTTGCCTTCGCTCGGTAT
GTGGAGCAGCGAGGCGCCGGGCTGACCGTGAGCCGTACCGATTACTCGGCCGATGTCTTC
GCGGAGCAGCTGTTCCGCGTGCTCCACGAGCCCTCGTTCCAGGACGGGGCGGCCGGACTT
CATGACGACATGCTGGCGACACCGAGCCCGCGTGACGTCGTGCCTGTCCTGGAGAAGCTG
TCCGACCGCTACCGCCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-134] 1.69999999999999e-24 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[211-307] 2.7e-29 PF06722
PF06722   DUF1205
SignalP
[1-23] 0.453 Signal
Bacteria, Gram-positive   
[1-28] 0.92 Signal
Eukaryota   
[1-23] 0.543 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Urd_00020 Urd_00020   Urd_00040 Urd_00040
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