Urd_00030 Urd_00030   Urd_00050 Urd_00050

Urd_00040 : CDS information

close this sectionLocation

Organism
StrainTü2717
Entry nameUrdamycin
Contig
Start / Stop / Direction4,629 / 5,795 / + [in whole cluster]
4,629 / 5,795 / + [in contig]
Location4629..5795 [in whole cluster]
4629..5795 [in contig]
TypeCDS
Length1,167 bp (388 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductD-olivose glycosyltransferase
Product (GenBank)glycosyl transferase
Gene
Gene (GenBank)urdGT1b
EC number
Keyword
  • C-9 trisaccharide
  • 3rd D-olivose
Note
Note (GenBank)
  • UrdGT1b
Reference
ACC
PmId
[10662691] Function of glycosyltransferase genes involved in urdamycin A biosynthesis. (Chem Biol. , 2000)
[11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000)
[11410375] Two sequence elements of glycosyltransferases involved in urdamycin biosynthesis are responsible for substrate specificity and enzymatic activity. (Chem Biol. , 2001)
comment
UniProtで登録されている[PMID: 10658661](2000)に、urdGT1bについての記載はない。

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[PMID: 10662691](2000)
[PMID: 11094336](2000)

urdamycin clusterに含まれるglycosyltransferase候補genes=urdGT1a, urdGT1b, urdGT1cについて、mutant作成、相補実験における蓄積産物解析を行い、各機能割り当てが確定されている。

UrdGT1bは、urdamycin A生合成において9-positionでの3糖側鎖3番目にあるD-olivoseの付着を担うO-glycosyltransferaseである。

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[PMID: 11410375](2001)
アミノ酸を91%共有しているUrdGT1b and UrdGT1cの基質特異性の違いに関する検討。
nucleotide sugarとacceptor substrateの特異性を決定するのはN末である。

close this sectionSequence

selected fasta
>D-olivose glycosyltransferase [glycosyl transferase]
MRVLMMSTPAPTHFTPLVSLAWALRGSGHEVVVAGQPDVLGAVASAGLNAVNVGDWFHVD
DMLVAGLREGERPLETRPRASLESMGGYGRVWMTHARYLVGRYMEFARIYGPDLIVSDPL
EYSSLLVGGVLGVPVVQQRWGVDLISGPARAEARPGFGPLCERLGLAGLPDPAVLLDPCP
PGLQAPGAEPGSPIRFVPFNGNGVVPGWLREPRSASRPRVVVTLGSRTLALNGVPLMRGI
LRAFEELPEVEAVATVDEVFREKVGPVPANVRMVDPVPLHLVLDGCAAVVHHGGAGTTMT
AGAFGLPQLVLPQLADQFGHGDRVSEVGAGISLDDAESQNDSHRLAVELRRLLAEPEFAK
AARALADSVRDMPAPAQVAADLTRLAGL
selected fasta
>D-olivose glycosyltransferase [glycosyl transferase]
ATGCGTGTACTGATGATGTCAACCCCCGCGCCGACGCATTTCACGCCGTTGGTGTCGTTG
GCGTGGGCCCTGCGGGGTTCGGGCCATGAGGTGGTGGTCGCGGGTCAGCCGGATGTACTG
GGGGCGGTCGCCTCGGCCGGGCTCAACGCGGTGAACGTCGGCGACTGGTTCCACGTGGAC
GACATGTTGGTCGCCGGGTTGCGGGAGGGGGAGCGCCCGCTCGAGACGCGTCCCCGTGCC
TCGCTCGAGTCGATGGGCGGGTACGGGCGGGTGTGGATGACCCATGCGCGTTATCTGGTC
GGGCGGTACATGGAGTTCGCTCGCATCTACGGTCCGGATCTGATCGTGTCGGATCCGCTG
GAGTACAGCTCGCTGCTGGTCGGGGGTGTGCTGGGGGTTCCGGTGGTGCAGCAGCGGTGG
GGGGTGGATCTGATCTCGGGTCCGGCGCGTGCGGAGGCGCGGCCGGGGTTCGGTCCGTTG
TGTGAGCGTCTGGGGCTTGCGGGGCTGCCGGATCCGGCGGTGTTGCTGGATCCGTGTCCG
CCGGGTCTGCAGGCTCCGGGGGCGGAGCCGGGCAGTCCGATCAGGTTCGTTCCGTTCAAC
GGCAACGGGGTGGTGCCGGGGTGGCTGCGCGAGCCGCGGTCCGCATCACGCCCCCGTGTA
GTCGTCACCCTGGGCAGTCGGACGTTGGCGTTGAACGGGGTGCCGTTGATGCGGGGCATT
CTGCGGGCGTTCGAGGAGCTGCCGGAGGTGGAGGCGGTGGCGACGGTGGACGAGGTGTTC
CGGGAGAAGGTCGGGCCGGTGCCGGCCAATGTGCGGATGGTCGATCCGGTGCCGCTGCAC
CTGGTGCTGGACGGATGTGCGGCCGTTGTGCACCACGGGGGCGCGGGTACGACGATGACC
GCCGGGGCGTTCGGGCTGCCCCAGCTGGTACTGCCCCAGCTCGCCGACCAGTTCGGGCAC
GGTGACCGGGTGAGCGAGGTGGGTGCCGGGATCTCGCTGGACGATGCGGAGAGCCAGAAC
GATTCGCATCGGCTGGCCGTGGAACTTCGGCGGCTGTTGGCGGAGCCGGAGTTCGCCAAG
GCCGCCCGCGCGCTGGCCGACTCGGTCCGGGACATGCCCGCACCCGCACAGGTCGCCGCC
GACCTCACACGGCTGGCCGGCCTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-56] 4.2e-09 PF03033
PF03033   Glyco_transf_28
IPR007235 Glycosyl transferase, family 28, C-terminal (Domain)
[282-364] 6.90000000000001e-05 PF04101
PF04101   Glyco_tran_28_C
IPR010610 Domain of unknown function DUF1205 (Domain)
[176-272] 1.2e-25 PF06722
PF06722   DUF1205
SignalP
[1-28] 0.964 Signal
Eukaryota   
[1-21] 0.516 Signal
Bacteria, Gram-negative   
[1-23] 0.536 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Urd_00030 Urd_00030   Urd_00050 Urd_00050
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