Urd_00180 : CDS information

Location

Organism	Streptomyces fradiae
Strain	Tü2717
Entry name	Urdamycin
Contig	AF164960
Start / Stop / Direction	20,435 / 21,688 / + [in whole cluster] 2,945 / 4,198 / + [in contig]
Location	20435..21688 [in whole cluster] 2945..4198 [in contig]
Type	CDS
Length	1,254 bp (417 aa)

Annotation

Category	4.3 transport
Product	putative major facilitator superfamily transporter
Product (GenBank)	transporter
Gene
Gene (GenBank)	urdJ2
EC number
Keyword
Note
Note (GenBank)	UrdJ2
Reference	ACC Q9RPA9 PmId [10658661] Two new tailoring enzymes, a glycosyltransferase and an oxygenase, involved in biosynthesis of the angucycline antibiotic urdamycin A in Streptomyces fradiae Tu2717. (Microbiology. , 2000) comment urdamycin生合成clusterの追加クローニング。 urdJ2については配列解析のみ。 UrdJ, UrdJ2はtransportersに似ている。 S.roseofulvus_putative export protein(AF058302)にid37%
Related Reference	ACC P42670 PmId [7916693] The pur8 gene from the pur cluster of Streptomyces alboniger encodes a highly hydrophobic polypeptide which confers resistance to puromycin. (Eur J Biochem. , 1993) comment Blast 219th, id41%, 8e-16, gapあり Streptomyces alboniger_pur8 Puromycin resistance protein pur8 puromycin合成clusterからpuromycin-resistance gene_pur8を分離し、Streptomyces lividansで発現させ、抗生物質抵抗性を検討。puromycinに高く特異的。 active puromycin effluxに加え、生合成経路の最終中間物であるN-acetylpuromycin分泌への関連があるかもしれない。 TMSs予測は14。上流ORFは逆向きで、抵抗性に関与しない。
Family/Domain	FD IPR011701 comment [IPR011701] Major facilitator superfamily (family)

Sequence

>putative major facilitator superfamily transporter [transporter]
MTTLSPRFDPHGRASAGPVARPRALLFVLAANMLIDALEVSVVLVALPAIGGDLGLSPQD
AQWMMSGFALGFAALLLSGPRITARWGRRRAYLVALLVFAVASSAGGLVHSGELLVLTRV
IKGMCAALTAPTGLAIIATAYRQGDEQRRAVAVYSFFGAAGFTVGLLASGALTVLSWRWD
LVLPAPIALGLMVLGFCLIPDDRGPAPTASPGTGVTRFLRHGPLVRSALCAASLNGAYLG
LLLLVTYQLHTGPGWNSWQTAVALLPACVPLMVSLPFAGRMVGRLGAARLIVSGTLAATL
GCAGCAVWGVSGSYATGALPALLLVEAGFVLSFAALNMQAVAGIAPESRQTAVSLYQTAV
QLGAALTLPAVALLLGSGGEGPYRTALLLITAVAAVGAAVACTGTRRKAEQPCVSRR

>putative major facilitator superfamily transporter [transporter]
ATGACGACTCTTTCCCCGCGCTTCGATCCGCACGGCCGCGCCTCGGCGGGACCGGTCGCA
CGGCCGCGGGCACTGCTGTTCGTGCTCGCGGCGAACATGCTCATCGACGCCCTGGAGGTC
TCGGTGGTGCTGGTCGCGCTGCCGGCCATCGGCGGCGATCTCGGCCTGTCACCCCAGGAC
GCCCAGTGGATGATGAGCGGCTTCGCACTCGGCTTCGCGGCACTGCTCCTGTCCGGCCCG
CGCATCACGGCACGGTGGGGGCGACGCCGTGCCTACCTGGTGGCGCTCCTCGTCTTCGCT
GTCGCATCCTCGGCAGGCGGATTGGTGCACAGCGGGGAGCTGCTCGTGCTCACCCGCGTC
ATCAAGGGCATGTGCGCCGCGCTCACCGCTCCGACCGGCCTCGCGATCATCGCCACCGCC
TACCGGCAGGGCGACGAGCAGCGCAGGGCGGTGGCGGTGTACTCGTTCTTCGGGGCCGCC
GGTTTCACCGTCGGTCTGCTGGCCTCGGGCGCACTCACCGTGCTGAGCTGGCGTTGGGAC
CTCGTCCTCCCGGCCCCGATCGCCCTGGGGCTGATGGTTCTGGGCTTCTGTCTCATCCCC
GACGACCGCGGGCCGGCGCCGACGGCTTCCCCCGGTACGGGGGTGACTCGATTCCTGCGG
CACGGCCCACTCGTGCGGTCGGCTCTGTGCGCGGCCAGCCTCAACGGTGCCTATCTCGGG
CTTCTCCTGCTGGTCACCTACCAACTGCACACGGGACCGGGCTGGAACTCCTGGCAGACG
GCGGTGGCTCTGCTGCCGGCCTGCGTGCCGCTGATGGTCTCGCTGCCCTTCGCCGGACGC
ATGGTGGGGCGCCTGGGCGCCGCCCGGCTGATCGTCTCGGGCACCCTCGCGGCCACGCTC
GGCTGCGCGGGCTGTGCCGTATGGGGTGTGTCCGGGTCGTACGCCACCGGTGCGCTGCCG
GCCCTGTTGCTCGTCGAGGCGGGATTCGTGCTGTCCTTCGCCGCTCTGAACATGCAGGCC
GTCGCCGGGATCGCACCGGAGTCACGGCAGACGGCGGTGTCCCTCTACCAGACGGCGGTG
CAACTCGGCGCCGCGCTGACGCTGCCGGCGGTCGCTCTGCTGCTGGGCAGCGGCGGCGAG
GGCCCCTACCGGACCGCCCTGCTCCTCATCACCGCCGTCGCCGCCGTCGGCGCCGCAGTC
GCCTGCACCGGGACGAGAAGAAAGGCGGAACAGCCATGCGTATCGAGGAGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011701 Major facilitator superfamily (Family) PF07690 MFS_1 IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain) SSF103473 MFS_gen_substrate_transporter IPR020846 Major facilitator superfamily domain (Domain) PS50850 MFS
SignalP	Eukaryota
TMHMM	Transmembrane 12

Urd_00170 Urd_00190