Urd_00190 : CDS information

Location

Organism	Streptomyces fradiae
Strain	Tü2717
Entry name	Urdamycin
Contig	AF164960
Start / Stop / Direction	21,670 / 22,293 / + [in whole cluster] 4,180 / 4,803 / + [in contig]
Location	21670..22293 [in whole cluster] 4180..4803 [in contig]
Type	CDS
Length	624 bp (207 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose epimerase
Product (GenBank)	epimerase
Gene
Gene (GenBank)	urdZ1
EC number	5.1.3.-
Keyword	L-rhodinose
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)	UrdZ1
Reference	ACC Q9RPA8 PmId [10658661] Two new tailoring enzymes, a glycosyltransferase and an oxygenase, involved in biosynthesis of the angucycline antibiotic urdamycin A in Streptomyces fradiae Tu2717. (Microbiology. , 2000) [11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000) comment [PMID: 10658661](2000) urdZ1は、8.05kbp fragmentから同定された7genesのうちのひとつ。 urdG, urdH and urdZ1はdeoxysugar生合成genesに似ている。 --- [PMID: 11094336](2000) urdZ3, urdQ and urdZ1をそれぞれ不活化するとL-rhodinoseを産生できない株となり、主にurdamycinone Bを蓄積。各genesで相補され、urdamycin A産生回復。よってこれらのgenesはNDP-L-rhodinose生合成に関与する。 urdamycin deoxysugar(NDP-D-olivose, NDP-L-rhodinose)生合成経路のFigあり。 UrdZ1 = NDP-D-cinerulose → NDP-L-cinerulose この機能的割り当てを確認するにはさらなる研究が必要。
Related Reference	ACC Q331R1 PmId [15049360] Cloning, expression, and biological function of a dTDP-deoxyglucose epimerase (gerF) gene from Streptomyces sp. GERI-155. (Biotechnol Lett. , 2004) [17053005] Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP. (Glycobiology. , 2007) comment Blast 70th, id39%, 6e-27 Streptomyces sp. KCTC 0041BP_gerF NDP-hexose-3-epimerase --- [PMID: 15049360](2004) Streptomyces sp. GERI-155 = Streptomyces sp. KCTC 0041BP dTDP-deoxyglucose epimerase geneをコードすると思われるgerFをE.coliで発現、精製、機能確認。精製された酵素はdTDP-4-keto-6-deoxyglucoseからmaltolを産生し、よって発現されたタンパクがdTDP-4-keto-6-deoxyglucoseのC-3 and C-5 or C-3の異性化を触媒するdTDP-deoxyglucose epimeraseであることが確認された。 --- [PMID: 17053005](2007) gerK1をクローニング、E.coliで過剰発現、機能確認。 GerK1はC-3でaxialなOH基を持つときのみ、dTDP-4-keto-6-deoxy-D-alloseの4-keto基で特異的な還元効果を示す。 GerFの存在下で、GerK1はdTDP-4-keto-6-deoxyglucose → dTDP-6-deoxy-beta-D-alloseへの変換を触媒した。このdTDP-6-deoxy-D-alloseの分離成功は、GerFがmycinose生合成経路において3,5-epimeraseではなく、3-epimeraseとして機能することを確認するのに十分な証拠を供給する。
Related Reference	ACC Q9S0P2 NITE Aver_00150 PmId [11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001) comment Blast 103rd, id39%, 2e-24 Streptomyces avermitilis_aveBV(avrF) dTDP-4-keto-6-deoxyhexose 3,5-epimerase [Aver_00150]dTDP-4-keto-6-deoxyhexose 5-epimerase Table1. avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　 (本文を読むと5-epimeraseの間違いと思われる。) S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。 delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。 avermectin A1a3を産生できるのはdelta-avrFのみ。 C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

Sequence

>putative NDP-hexose epimerase [epimerase]
MRIEEMSIPGAFLITPDQLSDERGAFYELRCDMLERAVGVPFQPQQINYSVSKRHTLRGI
HSVSIPPGQAKLVTCVRGALRDIVVDLRIGSPAFGAHQVTELDAVSGRSVYVPEVWGTDS
SRSPTTPVSATSSPSTYVPGTQIDINPLDPDLDLPWDCPQEPLISEKDAKASSLAEALAS
GTLPDLHDCRTSDAARTALVRAPSERQ

>putative NDP-hexose epimerase [epimerase]
ATGCGTATCGAGGAGATGAGCATCCCCGGTGCCTTCCTGATCACGCCGGATCAACTGTCC
GACGAGCGGGGTGCGTTCTACGAGCTGCGCTGCGACATGCTGGAGCGGGCCGTCGGCGTT
CCCTTCCAGCCCCAGCAGATCAACTACTCGGTCTCCAAGCGGCACACCCTGCGCGGCATC
CACAGCGTCAGCATCCCGCCCGGGCAGGCGAAGCTCGTCACCTGCGTGCGGGGAGCGCTG
CGCGACATCGTGGTCGACCTGCGGATCGGCTCTCCGGCCTTCGGCGCCCACCAGGTGACC
GAGCTGGACGCCGTCTCCGGTCGGTCCGTCTACGTACCCGAAGTGTGGGGCACGGATTCC
TCGCGCTCACCGACGACGCCTGTATCTGCTACGTCGTCTCCCAGCACCTACGTGCCAGGA
ACCCAGATCGACATCAATCCGCTCGATCCGGATCTCGACCTGCCCTGGGACTGTCCGCAG
GAACCGCTCATATCGGAGAAGGACGCGAAGGCGTCCAGCCTGGCCGAGGCACTGGCATCC
GGCACTCTGCCCGACCTGCACGACTGCCGAACCAGCGACGCCGCGCGCACAGCACTCGTC
CGTGCGCCATCCGAAAGGCAATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family) PF00908 dTDP_sugar_isom PD001462 Q9RPA8_STRFR_Q9RPA8; IPR011051 RmlC-like cupin domain (Domain) SSF51182 RmlC-like cupins IPR014710 RmlC-like jelly roll fold (Domain) G3DSA:2.60.120.10 no description
SignalP	No significant hit
TMHMM	No significant hit

Urd_00180 Urd_00200