Urd_00240 Urd_00240   Urd_00260 Urd_00260

Urd_00250 : CDS information

close this sectionLocation

Organism
StrainTü2717
Entry nameUrdamycin
Contig
Start / Stop / Direction27,922 / 28,665 / + [in whole cluster]
2,414 / 3,157 / + [in contig]
Location27922..28665 [in whole cluster]
2414..3157 [in contig]
TypeCDS
Length744 bp (247 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-4 ketoreductase
Product (GenBank)NDP-hexose 4-ketoreductase UrdR
Gene
Gene (GenBank)urdR
EC number
Keyword
  • D-olivose
Note
Note (GenBank)
Reference
ACC
PmId
[11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000)
[17434221] Surprising production of a new urdamycin derivative by S. fradiae Delta urdQ/R. (J Biotechnol. , 2007)
comment
[PMID: 11094336](2000)
urdamycinは、aglyconeへのNDP-D-olivose, NDP-L-rhodinose修飾の違いによってA,B,G,M,N,Oと名づけられている。そのNDP-D-olivose, NDP-L-rhodinoseの合成系(D-Glc-1-P=>=>)に関与する遺伝子群の実験(gene cloning, mutant作製)。

urdR不活化mutantは、9-positionでD-rhodinoseがC-glycosidicallyに付着したurdamycin Mを蓄積。
よってurdRは、NDP-D-olivose合成系に関与する。
rhodinoseは生合成できているので、共通中間体からのsingle-step processでD-olivoseに変換される必要がある。

以上から、
NDP-4-keto-2,6-dideoxy-D-glucose → NDP-D-olivose
の4-KR反応が割り当てられている。

---
[PMID: 17434221](2007)
urdQ/R double mutantの相補実験あり。

S. cyanogenus S136に由来するlandomycin生合成gene clusterの
dTDP-hexose-3,4-dehydrataseをコードするlanQと、
dTDP-hexose-4-ketoreductaseをコードするlanRでの相補は、
urdamycin A and urdamycin Bの産生をもたらした。

ただ、lanQ, lanR自体が実験的に機能確認されていないので微妙。

close this sectionSequence

selected fasta
>putative NDP-hexose C-4 ketoreductase [NDP-hexose 4-ketoreductase UrdR]
MDIVGNGFLARNLRPLAERHPDTVALAAGVSWASGTSDADFAREAALLRDTAKQCAATGR
RLLFFSTAATGMYGLAEGPGREDTPVTPCTPYGAHKLALEELLRDSGADHVILRLGHLVG
PDQPEHQLLPTLVRHLREGAVRIHRGAARDLIDVSDVVTIVDCLLGLDLKAETVNVASGY
AVPVKDIVELLRRRLGVEARYEFQDAGGQHVISIEKLRALVPQVQDMGFGPDYYRRILSD
FTSAVRI
selected fasta
>putative NDP-hexose C-4 ketoreductase [NDP-hexose 4-ketoreductase UrdR]
ATGGACATTGTGGGAAATGGATTCCTGGCGCGGAACCTGCGGCCGCTGGCCGAACGGCAT
CCCGATACCGTGGCCCTCGCCGCCGGTGTCTCATGGGCGAGCGGTACCTCCGATGCCGAC
TTCGCCCGGGAAGCCGCACTCCTGCGAGACACCGCCAAGCAGTGCGCGGCCACCGGACGA
CGACTGCTGTTCTTCTCGACCGCTGCCACGGGCATGTACGGCCTGGCGGAGGGACCCGGC
CGAGAGGACACGCCAGTGACGCCGTGCACCCCGTACGGCGCGCACAAGCTGGCGCTGGAG
GAACTGCTGCGGGACTCCGGTGCCGACCATGTGATTCTCAGGCTCGGGCACCTCGTGGGG
CCCGACCAGCCCGAGCACCAACTGCTGCCCACGCTGGTGCGTCACCTACGCGAAGGCGCG
GTGCGCATTCACCGAGGCGCGGCCCGTGACCTCATCGACGTCAGCGACGTCGTCACCATT
GTCGACTGCCTTCTCGGCCTTGACCTCAAGGCCGAGACGGTCAACGTGGCCTCCGGCTAC
GCCGTTCCGGTGAAGGACATCGTCGAACTCCTGCGCCGCAGGCTCGGGGTCGAGGCGCGG
TACGAGTTCCAGGATGCCGGTGGTCAGCACGTCATCTCCATCGAGAAGCTGCGCGCACTC
GTTCCGCAGGTCCAGGACATGGGCTTCGGGCCCGACTACTACCGGCGGATCCTCAGTGAC
TTCACCTCCGCCGTCCGTATCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[16-177] 4.5e-21 PF01370
PF01370   Epimerase
IPR016040 NAD(P)-binding domain (Domain)
[14-194] 2.1e-21 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-27] 0.224 Signal
Bacteria, Gram-positive   
[1-33] 0.22 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Urd_00240 Urd_00240   Urd_00260 Urd_00260
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