Urd_00260 : CDS information

Location

Organism	Streptomyces fradiae
Strain	Tü2717
Entry name	Urdamycin
Contig	AF269227
Start / Stop / Direction	28,704 / 30,113 / + [in whole cluster] 3,196 / 4,605 / + [in contig]
Location	28704..30113 [in whole cluster] 3196..4605 [in contig]
Type	CDS
Length	1,410 bp (469 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	NDP-hexose 2,3-dehydratase UrdS
Gene
Gene (GenBank)	urdS
EC number
Keyword	D-olivose L-rhodinose
Note
Note (GenBank)
Reference	ACC Q9L4S4 PmId [11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000) comment urdSの削除は遺伝子内へフレームシフト変異を導入することで達成され、fusion protein UrdS-UrdTの形成をもたらした。この株はurdamycin派生体を産生せず、wild株では通常検出されないdiketopiperazinesを蓄積した。 urdamycin deoxysugar(NDP-D-olivose, NDP-L-rhodinose)生合成経路のFigあり。 UrdSは両sugarsに共通するNDP-4-keto-2,6-dideoxy-D-glucose形成のために、 NDP-4-keto-6-deoxy-D-glucoseからの2,3-dehydrationが割り当てられている。参照されているのはgranaticin and oleandomycinでのdeoxyhexose生合成における2-deoxygenation stepに関する論文。
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 36th, id49%, 1e-121 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [NDP-hexose 2,3-dehydratase UrdS]
MLSESLLAVAQPRAADNTCDRIGLSAATADGRHLRTSDFGDWLAGRGRANEFRVDRVPFA
ALDGWAFDEATGNLVHRSGRFFTVEGLHVTELDAPYGDGPYADWHQPIIKQPEVGILGIL
VKEFDGVLHFLMQAKMEPGNPNLLQLSPTVQATRSNYTKAHHGADVKYIEHFVGPTRGHI
IADVLQSEHGSWFYRKSNRNMIVEAVGDVPLHDDFCWLTLGQISTLMHQDNVVNMDSRTV
LSCLPYADTRSRALLSDTQLLSWITEERSRHDVRVERVPLGSLPGWRRGESTIEHEAGRY
FKVVAVAVQAGSREVTGWTQPLFEPVGLGVTAFLTRAFDGVPHVLVHARVEAGFLDTIEL
GPTVQYTPGNYAHLDEKNRPLFLDTVLRAPSDRIRYEAVHSEEGGRFLNAESRYLIVDTD
ETEAPQNPPSGYAWVTPAQLTSLVRHGHYVNVQGRTLLACLNAMAVNSR

>putative NDP-hexose 2,3-dehydratase [NDP-hexose 2,3-dehydratase UrdS]
ATGCTTTCTGAAAGCCTTCTCGCAGTGGCCCAGCCGCGTGCGGCCGACAACACCTGTGAT
CGCATCGGGCTGTCCGCAGCGACCGCCGACGGTAGGCACCTGCGCACGTCGGATTTCGGG
GACTGGCTGGCCGGACGCGGCCGGGCCAACGAGTTCCGGGTGGACCGCGTACCGTTCGCC
GCACTGGACGGCTGGGCCTTCGACGAGGCCACCGGCAATCTGGTGCACCGCAGCGGCCGG
TTCTTCACAGTTGAGGGCCTGCACGTCACCGAGCTGGACGCCCCCTACGGCGACGGCCCC
TATGCCGACTGGCACCAGCCCATCATCAAGCAGCCCGAGGTGGGCATCCTGGGCATCCTG
GTCAAGGAGTTCGACGGGGTACTGCATTTTCTCATGCAGGCAAAGATGGAGCCGGGCAAT
CCGAACCTGCTCCAACTCTCGCCCACGGTTCAGGCCACTCGCAGCAACTACACCAAGGCC
CACCACGGAGCCGACGTGAAGTACATCGAGCACTTCGTCGGTCCGACGCGCGGTCACATC
ATCGCGGACGTACTGCAGTCGGAACACGGCTCGTGGTTCTACCGAAAGTCCAACCGCAAC
ATGATCGTGGAAGCCGTCGGCGACGTGCCGCTGCACGACGACTTCTGCTGGCTCACCCTC
GGCCAGATCAGCACGCTGATGCACCAGGACAACGTGGTCAACATGGACTCGCGTACCGTC
CTGTCCTGCCTGCCCTACGCGGACACCCGTTCGCGCGCGCTGCTCTCCGACACCCAGTTG
CTCTCCTGGATCACCGAGGAGCGATCGCGACACGATGTGCGGGTTGAGCGGGTCCCGCTC
GGGAGCCTCCCGGGGTGGCGACGCGGTGAGAGCACGATCGAGCACGAAGCGGGCCGGTAC
TTCAAGGTCGTGGCCGTGGCCGTGCAGGCCGGCAGCCGTGAGGTGACCGGCTGGACCCAG
CCCCTCTTCGAGCCGGTCGGCCTGGGCGTCACGGCCTTCCTCACCCGCGCATTCGATGGC
GTTCCGCACGTGCTGGTTCACGCCCGGGTCGAGGCCGGTTTCCTCGACACCATCGAGCTG
GGCCCGACCGTCCAGTACACCCCGGGCAATTACGCCCACCTGGACGAGAAGAACCGGCCG
CTCTTCCTCGACACCGTGCTGCGTGCCCCGTCGGATCGCATCCGCTACGAGGCCGTGCAC
TCGGAGGAGGGCGGTCGCTTCCTCAACGCCGAGAGCCGCTACCTGATCGTCGACACCGAC
GAGACGGAGGCCCCGCAGAACCCGCCGTCGGGCTACGCCTGGGTCACACCGGCACAACTG
ACCTCGCTGGTGCGACACGGCCATTACGTCAATGTCCAGGGCCGCACTCTGCTGGCCTGC
CTCAACGCCATGGCGGTGAACAGCCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	No significant hit
TMHMM	No significant hit

Urd_00250 Urd_00270