Urd_00260 Urd_00260   last last

Urd_00270 : CDS information

close this sectionLocation

Organism
StrainTü2717
Entry nameUrdamycin
Contig
Start / Stop / Direction30,110 / 30,916 / + [in whole cluster]
4,602 / 5,408 / + [in contig]
Location30110..30916 [in whole cluster]
4602..5408 [in contig]
TypeCDS
Length807 bp (268 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 ketoreductase(fragment)
Product (GenBank)oxidoreductase UrdT
Gene
Gene (GenBank)urdT
EC number
Keyword
  • D-olivose
  • L-rhodinose
Note
Note (GenBank)
Reference
ACC
PmId
[11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000)
comment
urdamycin deoxysugar(NDP-D-olivose, NDP-L-rhodinose)生合成経路のFigあり。
UrdTは両sugarsに共通するNDP-4-keto-2,6-dideoxy-D-glucose形成のために、
UrdSの2,3-dehydrationに続くreductionが割り当てられている。
参照されているのはgranaticin and oleandomycinでのdeoxyhexose生合成における2-deoxygenation stepに関する論文。
Related Reference
ACC
Q9ALN5
NITE
Spino_00080
PmId
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
Blast 13th, id51%, 1e-61, C末66aa長い
Saccharopolyspora spinosa_spnN
Probable NDP-hexose-3-ketoreductase

NAD(P)H-dependent dTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductase_SpnN

dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), TylX3(SpnNの1つ前の反応を担うSpnOのhomologue), SpnN, and NADPHをインキュベーションしてできた産物がdTDP-4-keto-2,6-dideoxy-D-glucoseであることをHPLC chromatogram、1H NMR spectrumなどで確認している。NADPHはNADHの2倍速く消費されるので、おそらくNADPH-dependentである。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 ketoreductase(fragment) [oxidoreductase UrdT]
MSRPVGIGVIGCADIAVRRMLPAFATGPETEVVAVASRTLGKAERVAERFGCRAVQGYAA
LLELEKVQAVYIPLPAALHAEWVRKAALRGQSTCWPRNPSRPTPLRTEQLLKLARYRGLA
LMENVMFVHHSRHEAVRRLVDEGAIGELRGLQSAFTIPAPADDDIRYAPELGGGALPDVG
VYPLRAALHFLGPGLEVVGAHLASGPGRHVETSGAALLRTPAGVTAQVIFGMEHTYRSTY
YLLGGDGRLTVERAFTPSADFIPVIPVPP
selected fasta
>putative NDP-hexose C-3 ketoreductase(fragment) [oxidoreductase UrdT]
ATGAGCCGACCGGTGGGGATCGGAGTGATCGGCTGTGCCGACATCGCGGTACGCCGGATG
CTCCCTGCTTTCGCAACCGGGCCTGAGACAGAGGTCGTCGCGGTTGCCAGCCGCACCCTG
GGCAAGGCCGAGCGGGTTGCCGAGCGCTTCGGGTGCCGGGCTGTCCAGGGCTACGCCGCG
CTCCTGGAACTGGAGAAGGTGCAGGCCGTGTACATACCGCTGCCTGCCGCTCTGCACGCG
GAGTGGGTCCGAAAGGCTGCGCTGCGCGGGCAAAGCACGTGCTGGCCGAGAAACCCCTCA
CGCCCGACCCCGCTGCGCACTGAGCAGCTGCTGAAGCTGGCCCGGTACCGCGGGCTGGCC
CTGATGGAGAACGTCATGTTCGTCCATCATTCTCGCCATGAGGCGGTTCGGCGGCTGGTC
GACGAGGGCGCGATCGGTGAACTGCGCGGTCTGCAGTCGGCATTCACCATTCCGGCTCCG
GCCGACGACGACATCCGGTACGCCCCGGAGCTCGGCGGCGGCGCGCTCCCGGACGTCGGC
GTCTATCCGCTCCGCGCCGCCTTGCACTTCCTCGGCCCGGGGCTGGAAGTCGTCGGAGCA
CATCTCGCGAGTGGACCCGGCAGACACGTCGAGACCTCGGGCGCGGCCCTGCTGCGTACA
CCTGCGGGCGTCACGGCGCAGGTGATCTTTGGCATGGAGCACACGTACCGCTCCACCTAT
TATCTGTTGGGCGGTGACGGACGGCTCACCGTCGAGCGGGCCTTCACACCGTCGGCCGAC
TTCATTCCCGTGATCCCCGTCCCCCCG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000683 Oxidoreductase, N-terminal (Domain)
[6-91] 3.8e-14 PF01408
PF01408   GFO_IDH_MocA
IPR004104 Oxidoreductase, C-terminal (Domain)
[137-240] 2.8e-05 PF02894
PF02894   GFO_IDH_MocA_C
IPR016040 NAD(P)-binding domain (Domain)
[1-147] 3.2e-29 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-25] 0.151 Signal
Bacteria, Gram-negative   
[1-25] 0.235 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
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