Acla_00010 Acla_00010   Acla_00030 Acla_00030

Acla_00020 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction1,261 / 656 / - [in whole cluster]
1,261 / 656 / - [in contig]
Locationcomplement(656..1261) [in whole cluster]
complement(656..1261) [in contig]
TypeCDS
Length606 bp (201 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose epimerase
Product (GenBank)AknL
Gene
Gene (GenBank)aknL
EC number5.1.3.-
Keyword
  • L-rhodosamine
  • L-2-deoxyfucose
  • L-rhodinose
Note
Note (GenBank)
  • dTDP-4-keto-6-deoxyhexose 3,5-epimerase
Reference
ACC
PmId
[12137949] Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. (Gene. , 2002)
comment
aclacinomycin PKSを含んだ13.5kb領域のクローニング、シークエンス報告論文。

deoxysugar生合成に参加するepimerase (aknL)

配列解析のみ。
dTDP-4-keto-6-deoxy-D-glucose→dTDP-4-keto-L-rhamnoseへの変換が提唱されているDnmUに高い類似性あり。
Related Reference
ACC
Q9S0P2
NITE
Aver_00150
PmId
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
Blast 21st, id58%, 3e-58
Streptomyces avermitilis_aveBV(avrF)
dTDP-4-keto-6-deoxyhexose 3,5-epimerase
[DoBISCUIT]NDP-hexose 5-epimerase

Table1.
avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　
(本文を読むと5-epimeraseの間違いと思われる。)

S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。
avermectin A1a3を産生できるのはdelta-avrFのみ。
C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

close this sectionSequence

selected fasta
>putative NDP-hexose epimerase [AknL]
MQFRELAVSGAFVFTPPVFEDDRGLFTSPFQEPAFVAALGHPLFPVAQSNHSRSRRGTVR
GIHYTVTPPGVAKYVYCARGRAIDIVVDIRVGSPTYGRWDSSVLDPEGFRAMYFPVGVGH
AFVALEDDTVMSYMLSGSYEAQHEMSLSPLDPALGLPIPQDVAPLLSARDTAAPLLEQVR
AEGGLPEYDKCRRIEAALWRP
selected fasta
>putative NDP-hexose epimerase [AknL]
GTGCAGTTCCGCGAACTCGCCGTCTCCGGCGCGTTCGTCTTCACTCCGCCCGTCTTCGAG
GACGACCGCGGTCTGTTCACCTCGCCGTTCCAGGAACCTGCCTTCGTGGCGGCACTGGGC
CACCCCCTGTTCCCGGTCGCGCAGTCCAACCACAGCAGGTCGCGGCGCGGCACCGTACGG
GGCATCCACTACACGGTCACCCCGCCGGGCGTGGCGAAGTACGTGTACTGCGCCCGGGGC
CGCGCCATCGACATCGTGGTCGACATCCGCGTCGGCTCGCCCACCTACGGCCGCTGGGAC
TCCTCGGTGCTCGACCCGGAGGGGTTTCGCGCCATGTACTTCCCGGTCGGCGTCGGGCAC
GCCTTCGTCGCCCTGGAGGACGACACGGTGATGTCGTACATGCTCTCGGGAAGCTACGAG
GCGCAGCACGAGATGTCCCTCTCCCCGCTCGACCCCGCGCTCGGCCTGCCGATCCCCCAG
GACGTGGCACCCCTGCTGTCCGCGCGGGACACGGCCGCGCCCCTGCTCGAGCAGGTGCGG
GCGGAGGGCGGCCTGCCGGAGTACGACAAGTGCCGTCGGATCGAGGCCGCACTGTGGCGG
CCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family)
[1-183] PD001462
PD001462   dTDP_sugar_isom
[4-178] 1.2e-55 PF00908
PF00908   dTDP_sugar_isom
IPR011051 RmlC-like cupin domain (Domain)
[1-183] 2.2999842048857e-66 SSF51182
SSF51182   RmlC_like_cupin
IPR014710 RmlC-like jelly roll fold (Domain)
[1-191] 4.39999999999997e-58 G3DSA:2.60.120.10
G3DSA:2.60.120.10   RmlC-like_jellyroll
SignalP
[1-20] 0.606 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Acla_00010 Acla_00010   Acla_00030 Acla_00030
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