Acla_00030 : CDS information

Location

Organism	Streptomyces galilaeus
Strain	ATCC 31615
Entry name	Aclacinomycin A
Contig	AF257324
Start / Stop / Direction	2,596 / 1,274 / - [in whole cluster] 2,596 / 1,274 / - [in contig]
Location	complement(1274..2596) [in whole cluster] complement(1274..2596) [in contig]
Type	CDS
Length	1,323 bp (440 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	L-2-deoxyfucose glycosyltransferase
Product (GenBank)	AknK
Gene
Gene (GenBank)	aknK
EC number
Keyword
Note
Note (GenBank)	glycosyl transferase
Reference	ACC Q9L555 PmId [12137949] Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. (Gene. , 2002) [15078101] AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A. (Biochemistry. , 2004) [17685523] Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A. (J Am Chem Soc. , 2007) comment [PMID: 12137949](2002) aclacinomycin PKSを含んだ13.5kb領域のクローニング、シークエンス報告論文。 deoxysugar生合成に参加するglycosyl transferase (aknK) 配列解析のみ。 -- [PMID: 15078101](2004) abstract AknKを異種性発現して精製、実験に使用。 dTDP-L-2-deoxyfucoseとrhodosaminyl aklavinoneから、AknKはL-2-deoxyfucosyl-L-rhodosaminyl aklavinoneを生成。 dTDP-L-daunosamineなどでも作用し、anthracycline骨格で2糖目を組み立てる。活性は下がるが、rhodosaminyl aklavinoneにdTDP-L-2-deoxyfucoseをtandemに追加して、L-deoxyfucosyl-L-deoxyfucosyl-L-rhodosaminyl aklavinoneを産生することもできる。 -- [PMID: 17685523](2007) AknSはゆっくりな基質としてdTDP-2-deoxyfucoseを受け入れるけど、AknKはdTDP-L-rhodosamineを利用できない。

Sequence

>L-2-deoxyfucose glycosyltransferase [AknK]
MKVLFTTFAAKSHMHAQVPLAWALQTAGHEVRIASQPDLAEDITRTGLTAVCVGEPLLLE
EQMQRVNEGLGDDAEIMESQAEAGMDMTETRPEMLTWDHVLGVFTSMTAMAFQNSCPERM
IDDVVAFAREWQPDLIVWDTLSFAGPVAAQVTGAAHARLLFGLDLLGRMRETFLDLQEER
LPEQRDDPLREWLTWTLGRYGAEFEEEVAVGQWTVDPVPPSMRFPVKQPFVPLRYIPYNG
QAVIPDWLHEPPKKRRVCLTLGVAHREVLDGDRASIGELVEALAELDVEVVATLNEKQLA
GMELPDNVRAVDFVPLNALLPTCSAVIHHGGSGTFQTALAHGVPQLIVPDMVWDTIHKAK
QLERFGAGLYLHDVDNYTAQDLRDHLLRLLEEPSFAENCARIRREMVGTPSPNDIVPLLE
KLTAEHRRDRGARGTVRGEQ

>L-2-deoxyfucose glycosyltransferase [AknK]
ATGAAGGTCCTGTTCACCACGTTCGCCGCGAAGTCCCATATGCATGCCCAGGTTCCGCTG
GCCTGGGCCCTCCAGACCGCGGGCCACGAGGTCCGTATCGCCAGCCAGCCGGACCTGGCG
GAGGACATCACCCGTACCGGACTGACCGCCGTGTGTGTCGGTGAGCCGCTGCTCCTGGAG
GAGCAGATGCAGCGGGTCAACGAGGGCCTGGGCGACGACGCCGAGATCATGGAGAGCCAG
GCGGAAGCCGGGATGGACATGACGGAGACCCGTCCCGAGATGCTGACCTGGGACCACGTC
CTCGGTGTGTTCACCTCGATGACGGCGATGGCCTTCCAGAACTCCTGCCCTGAGCGCATG
ATCGACGACGTGGTCGCGTTCGCCCGCGAGTGGCAGCCCGACCTGATCGTCTGGGACACG
CTGTCCTTCGCGGGGCCGGTGGCCGCGCAGGTCACCGGCGCCGCCCACGCCCGGCTGCTG
TTCGGCCTCGATCTGCTGGGGCGGATGCGGGAGACCTTCCTCGACCTTCAGGAGGAGCGG
CTGCCCGAGCAGCGGGACGACCCGCTGCGCGAGTGGCTGACCTGGACGCTCGGCCGCTAC
GGCGCCGAGTTCGAGGAGGAGGTGGCGGTCGGTCAGTGGACCGTCGACCCGGTGCCGCCG
TCGATGCGGTTCCCGGTGAAGCAGCCGTTCGTGCCACTGCGGTACATCCCCTACAACGGG
CAGGCCGTCATCCCGGACTGGCTGCACGAGCCGCCGAAGAAGCGCCGCGTCTGTCTGACG
CTGGGGGTGGCCCACCGCGAGGTGCTGGACGGCGACCGCGCCTCGATCGGCGAACTCGTC
GAGGCGCTCGCGGAACTGGACGTCGAGGTGGTCGCGACGCTCAACGAGAAGCAGCTCGCC
GGGATGGAACTGCCGGACAACGTCAGGGCCGTCGACTTCGTCCCGCTCAACGCGCTGCTG
CCGACCTGCTCCGCGGTCATCCACCACGGTGGCTCCGGCACCTTCCAGACGGCGCTGGCG
CATGGTGTCCCGCAGCTCATCGTGCCCGACATGGTCTGGGACACGATCCACAAGGCGAAG
CAGCTCGAGCGGTTCGGGGCGGGGCTCTACCTGCACGACGTCGACAACTACACCGCTCAG
GACCTGCGCGACCACCTGCTGCGGCTGCTGGAGGAGCCGTCGTTCGCAGAGAACTGCGCG
CGTATCCGCCGGGAGATGGTGGGCACGCCGAGCCCCAACGACATCGTCCCGCTGCTGGAG
AAGCTCACCGCGGAACACCGCCGTGACCGAGGTGCCCGTGGCACCGTCCGAGGGGAGCAG
TAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Eukaryota
TMHMM	No significant hit

Acla_00020 Acla_00040