Acla_00040 : CDS information

Location

Organism	Streptomyces galilaeus
Strain	ATCC 31615
Entry name	Aclacinomycin A
Contig	AF257324
Start / Stop / Direction	4,034 / 2,682 / - [in whole cluster] 4,034 / 2,682 / - [in contig]
Location	complement(2682..4034) [in whole cluster] complement(2682..4034) [in contig]
Type	CDS
Length	1,353 bp (450 aa)

Annotation

Category	3.4 other modification
Product	putative cyclase/dehydratase
Product (GenBank)	AknE1
Gene
Gene (GenBank)	aknE1
EC number
Keyword	1st ring
Note
Note (GenBank)	putative bifunctional aromatase
Reference	ACC Q9L554 PmId [12137949] Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. (Gene. , 2002) comment aclacinomycin PKSを含んだ13.5kb領域のクローニング、シークエンス報告論文。 AknE1: aromatase, putative bifunctional 配列解析のみ。 type II PKS clusterに見られるaromatasesよりC末が130aa長く、この部分は機能不明なDpsH(DnmW)にid44%あり。 Akn clusterには他にDpsH homologueあり(AknV)。 AknE1と同じように長いタイプのaromoataseもS.purpurascensのrhodomycin clusterにある(rdmK)。
Related Reference	ACC Q54811 NITE Adria_00240 PmId [9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997) comment Blast 14th, id56%, 6e-88, C末足りない Streptomyces peucetius_dpsF Daunorubicin-doxorubicin polyketide synthase [DoBISCUIT]cyclase/dehydrase minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。 JadD, DpsFの両cyclasesはC-9で還元されたdecaketidesの位置特異的なfirst-ring cyclase/dehydrasesであることが示された。

Sequence

>putative cyclase/dehydratase [AknE1]
MAHEVHTTEHTITVAAAPEAVFGLVERAEDWPHVFPPSLHVEYLERSGEEERLRIWATAN
GEVKSWTSRRTLDRAGLRIRFRQEVSQDPVAAMGGEWIVEALPGGGSRVRLLHDFRAVDD
LERNVSWILEAIERNSGAELAALESAALRTGTDEAPYTFEDSVRIDAPAQDVRDFLHDAG
QWQERLPHVARVLLKEDTPDVQHLEMDTRTPDGSTHTTASVRICLPDGRITYKQLQTPAL
MSVHTGEWSVREIPSGCVAVSRHTVVVNAAAVTKVLGASATTADARTFIRDALGGNSTIT
MRHAKEYAQRRAATEPVPPAEYASVQRFYARQMRLLDEGEGDAWADTFTEDGTFDQSSFA
EPLRGRAAIAAAVRGRPAAPAGTVRRHWLGLPAAWRFPDGSVRTAYDALVVSTEAGAAPV
IRLSTSCQDVLVADGDGWLVSHRYVGHDGQ

>putative cyclase/dehydratase [AknE1]
ATGGCGCACGAGGTGCACACGACCGAACACACGATCACCGTGGCCGCCGCCCCGGAGGCC
GTCTTCGGCCTGGTGGAGCGGGCGGAGGACTGGCCGCACGTCTTTCCGCCGAGCCTGCAC
GTGGAGTATCTGGAGCGGTCGGGCGAGGAGGAGCGGCTGCGCATCTGGGCCACCGCCAAC
GGCGAGGTCAAGTCCTGGACCTCACGGCGCACCCTGGACCGCGCGGGACTGCGGATCCGG
TTCCGCCAGGAGGTCTCCCAGGATCCCGTAGCCGCGATGGGCGGGGAGTGGATCGTCGAG
GCGCTCCCCGGCGGCGGCAGCCGGGTCCGGCTGCTGCACGACTTCCGGGCCGTCGACGAC
CTGGAACGCAACGTCAGCTGGATCCTGGAGGCGATCGAGCGCAACAGCGGGGCCGAGCTG
GCCGCGCTGGAGTCCGCCGCGCTGCGCACCGGCACCGACGAGGCGCCCTACACCTTCGAG
GACTCGGTGCGCATCGACGCCCCGGCCCAGGACGTGCGGGACTTCCTGCACGACGCGGGG
CAGTGGCAGGAACGGCTGCCGCACGTCGCCCGGGTGCTGCTGAAGGAGGACACGCCCGAC
GTCCAGCACCTGGAGATGGACACCCGCACCCCCGACGGTTCGACGCACACCACGGCTTCG
GTGCGGATCTGCCTGCCCGACGGGCGGATCACGTACAAGCAGCTGCAGACGCCGGCCCTG
ATGTCCGTGCACACCGGGGAGTGGTCGGTACGGGAGATCCCGTCCGGCTGCGTGGCCGTC
TCCCGGCACACCGTGGTCGTCAACGCGGCGGCCGTCACCAAGGTCCTCGGTGCCTCCGCG
ACGACCGCCGACGCCCGCACCTTCATCCGGGACGCGCTCGGCGGCAACAGCACGATCACC
ATGCGGCACGCCAAGGAGTACGCGCAGCGGCGGGCCGCGACGGAGCCGGTGCCGCCGGCC
GAGTACGCCTCGGTCCAGCGGTTCTACGCCCGTCAGATGCGGCTCCTCGACGAGGGCGAG
GGGGATGCCTGGGCGGACACCTTCACCGAGGACGGGACCTTCGACCAGAGCTCGTTCGCC
GAGCCGCTGCGCGGCCGGGCGGCGATCGCGGCGGCGGTGCGCGGCCGGCCCGCGGCGCCC
GCCGGCACGGTGCGCCGGCACTGGCTGGGCCTGCCGGCCGCCTGGCGGTTCCCGGACGGT
TCGGTGCGCACGGCCTACGACGCCCTGGTCGTCTCGACCGAGGCGGGCGCCGCTCCGGTG
ATCCGGCTGAGCACCTCGTGCCAGGACGTCCTCGTCGCCGACGGGGACGGCTGGCTGGTG
AGCCACCGCTACGTCGGCCACGACGGGCAGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR019587 Polyketide cyclase/dehydrase (Family) PF10604 Polyketide_cyc2 IPR023393 START-like domain (Domain) G3DSA:3.30.530.20 G3DSA:3.30.530.20
SignalP	Eukaryota
TMHMM	No significant hit

Acla_00030 Acla_00050