Acla_00040 Acla_00040   Acla_00060 Acla_00060

Acla_00050 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction4,867 / 4,082 / - [in whole cluster]
4,867 / 4,082 / - [in contig]
Locationcomplement(4082..4867) [in whole cluster]
complement(4082..4867) [in contig]
TypeCDS
Length786 bp (261 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative polyketide C-9 ketoreductase
Product (GenBank)AknA
Gene
Gene (GenBank)aknA
EC number
Keyword
Note
Note (GenBank)
  • polyketide ketoreductase
Reference
ACC
PmId
[12137949] Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. (Gene. , 2002)
comment
aclacinomycin PKSを含んだ13.5kb領域のクローニング、シークエンス報告論文。

AknA: polyketide ketoreductase (KRI)

S.galilaeusの株違い3AR-33のKRIとほとんど同じ。
KRI はpolyketide鎖のC2でketo基→OH基へ還元する。
そのOH基は、AROによる最初の環のclosing and aromatisingのときに除去される。

間違った折りたたみをされたpolyketideを産生するS.galilaeus polyketide KR不全mutant H061は、plasmid pSgs15(aknLKE1AXBCDE2F)の導入でaclacinomycin産生を回復した。
Related Reference
ACC
Q8VWB5
PmId
[8157619] Nucleotide sequence of the aknA region of the aklavinone biosynthetic gene cluster of Streptomyces galilaeus. (J Bacteriol. , 1994)
comment
Blast 2nd, id98%, 1e-145
Streptomyces galilaeusの株違い3AR-33での報告。

2-hydroxyaklavinone(polyketide C9の位置にOH基が残っているaklavinone)を産生するS. galilaeus ANR-58では、aknAの配列に変更がある。
ACC
Q56166
NITE
Jado_00110
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 21st, id73%, 1e-104
Streptomyces venezuelae_ORF5(jadE)
Ketoreductase

--
[PMID: 7881555](1994)
ORF5(=jadEに相当)は配列解析からketoreductaseであると提唱されている。
ORF2-ORF4領域(ORF5も含まれる)を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、
そのplasmid発現株での産物を解析。
JadEはdecaketideだけでなく、octaketideも還元できる。
ACC
Q54812
NITE
Adria_00250
PmId
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 26th, id70%, 1e-102
Streptomyces peucetius_dpsE
Daunorubicin-doxorubicin polyketide synthase
[DoBISCUIT]C-9 ketoreductase

DpsEは、DpsFによる環化に先立って必要なC-9還元を担う、decaketideに特異的なketoreductaseであることが実験で確認されている。

minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
DpsE KRはdecaketidesを還元できるが、octaketidesを還元できない。

close this sectionSequence

selected fasta
>putative polyketide C-9 ketoreductase [AknA]
MPPASERVAIVTGATSGIGLAVARSLAEGGARVFICARDGDRVAHTVKELREAGHDVDGA
SCDVRDTARVRAFVQEARDRFGPVDALVNNAGRSGGGHTAQIADELWLDVIETNLNSVFR
MTREVLTTGGMLERGAGRIVNIASTGGKQGVALGAPYSASKHGVVGFTKALGLELAKTGI
TVNAVCPGYVETPMAERVRQGYAGAWDITEDEVLERFEAKIPLGRYSTPDEVAGLVGYLL
SSTAASITAQAMNVCGGLGNY
selected fasta
>putative polyketide C-9 ketoreductase [AknA]
ATGCCGCCTGCATCAGAACGCGTCGCCATCGTCACGGGTGCCACCAGCGGCATCGGTCTC
GCCGTGGCCAGGAGCCTGGCCGAAGGCGGTGCGCGGGTCTTCATCTGCGCCCGCGACGGC
GACCGGGTCGCCCACACCGTCAAGGAGTTGCGCGAGGCGGGCCACGACGTCGACGGCGCC
TCCTGCGACGTACGCGACACCGCGCGGGTACGCGCCTTCGTCCAGGAGGCCAGGGACCGG
TTCGGGCCCGTCGACGCCCTGGTGAACAACGCCGGCCGCAGCGGCGGCGGGCACACCGCG
CAGATTGCCGACGAGTTGTGGCTCGACGTGATCGAGACCAACCTCAACAGCGTCTTCCGG
ATGACCCGCGAGGTGCTGACGACCGGCGGGATGCTGGAGCGCGGCGCCGGCCGGATCGTC
AACATCGCCTCGACGGGCGGCAAGCAGGGCGTGGCCCTGGGGGCGCCGTACTCGGCGTCG
AAGCACGGCGTGGTCGGCTTCACCAAGGCCCTGGGCCTCGAGCTGGCGAAGACGGGCATC
ACCGTCAACGCGGTCTGCCCGGGTTACGTCGAGACACCGATGGCCGAGCGGGTGCGCCAG
GGATACGCCGGCGCCTGGGACATCACCGAGGACGAGGTGCTGGAGAGGTTCGAGGCGAAG
ATCCCCCTCGGCCGCTACTCCACGCCGGACGAGGTCGCCGGACTCGTCGGCTACCTGCTC
TCCTCGACGGCCGCGTCGATCACCGCCCAGGCGATGAACGTCTGCGGCGGGCTCGGCAAC
TACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[82-93] 4.99999811956429e-14 PR00080 [137-145] 4.99999811956429e-14 PR00080 [157-176] 4.99999811956429e-14 PR00080
PR00080   SDRFAMILY
[3-248] 2.00000075217457e-16 PIRSF000126
PIRSF000126   11-beta-HSD1
[8-175] 1.09999999999999e-37 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[8-25] 4.39999001237725e-48 PR00081 [82-93] 4.39999001237725e-48 PR00081 [131-147] 4.39999001237725e-48 PR00081 [157-176] 4.39999001237725e-48 PR00081 [178-195] 4.39999001237725e-48 PR00081 [222-242] 4.39999001237725e-48 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[6-258] 1.69999999999998e-82 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[144-172] PS00061
PS00061   ADH_SHORT
SignalP
[1-27] 0.891 Signal
Bacteria, Gram-positive   
[1-27] 0.52 Signal
Bacteria, Gram-negative   
[1-23] 0.727 Signal
Eukaryota   
TMHMM No significant hit
Acla_00040 Acla_00040   Acla_00060 Acla_00060
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