Acla_00050 Acla_00050   Acla_00070 Acla_00070

Acla_00060 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction5,079 / 5,447 / + [in whole cluster]
5,079 / 5,447 / + [in contig]
Location5079..5447 [in whole cluster]
5079..5447 [in contig]
TypeCDS
Length369 bp (122 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative 12-deoxyaklanonic acid C-12 oxygenase
Product (GenBank)AknX
Gene
Gene (GenBank)aknX
EC number
Keyword
  • anthraquinone
Note
Note (GenBank)
  • polyketide oxygenase
Reference
ACC
PmId
[12137949] Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. (Gene. , 2002)
comment
aclacinomycin PKSを含んだ13.5kb領域のクローニング、シークエンス報告論文。

AknX: mono-oxygenase

配列解析のみ。
anthracycline polyketidesのC12で酸素を添加し、aklanonic acidを形成すると提唱。
Related Reference
ACC
Q8VWB4
PmId
[12399480] Expression, purification, and characterization of AknX anthrone oxygenase, which is involved in aklavinone biosynthesis in Streptomyces galilaeus. (J Bacteriol. , 2002)
comment
Streptomyces galilaeusの株違い3AR-33

Blast 2nd, id98%, 1e-62
Streptomyces galilaeus_aknX
AknX

AknXをE.coliで過剰発現、精製、酵素活性測定。
emodinanthrone → anthraquinone emodinへ変換するanthrone oxygenase activity確認あり。
TcmHのようなoxygen activationのための補欠分子族を持たないので、peroxy anion intermediateを介した異なるメカニズムが提唱されている。

anthranol anionの形成で反応は始まり、それは固定された向きでTrp-67とのπ-π複合体形成によって安定化される。それからhydroperoxyanthroneがdioxygen添加によって形成され、続いてanthraquinone形成のためにdehydrateされる、と提唱されている(Fig9)。

close this sectionSequence

selected fasta
>putative 12-deoxyaklanonic acid C-12 oxygenase [AknX]
MTDHEPGTEGADAVTFVNTFTVHAEPEVFEKEFARTSEFMAQQPGFVRHTLCRHAERPGQ
YVNVAEWRDLASFRAAVSHDGFRPHADALRALSESRPELYLVRLRREGAPGLDGPASEGE
EI
selected fasta
>putative 12-deoxyaklanonic acid C-12 oxygenase [AknX]
ATGACGGATCATGAGCCAGGCACCGAGGGGGCCGACGCCGTGACCTTCGTGAACACCTTC
ACCGTGCACGCCGAACCCGAGGTGTTCGAGAAGGAGTTCGCGCGGACGTCGGAGTTCATG
GCACAGCAGCCGGGTTTCGTCCGGCACACGCTCTGCCGGCACGCGGAGCGGCCCGGCCAG
TACGTCAACGTCGCCGAGTGGCGGGACCTCGCCTCGTTTCGCGCGGCCGTCTCGCACGAC
GGCTTCCGGCCGCACGCCGACGCGCTGCGCGCGCTGAGCGAGAGCCGGCCCGAACTGTAT
CTGGTACGGCTGCGCCGCGAGGGCGCCCCGGGTCTCGACGGTCCGGCGTCCGAAGGGGAG
GAGATATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR007138 Antibiotic biosynthesis monooxygenase (Domain)
[14-87] 9.29999999999998e-18 PF03992
PF03992   ABM
IPR011008 Dimeric alpha-beta barrel (Domain)
[4-106] 1.79999754022375e-18 SSF54909
SSF54909   Dimer_A_B_barrel
SignalP
[1-24] 0.036 Signal
Eukaryota   
[1-24] 0.175 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Acla_00050 Acla_00050   Acla_00070 Acla_00070
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