Acla_00060 Acla_00060   Acla_00080 Acla_00080

Acla_00070 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction5,444 / 6,715 / + [in whole cluster]
5,444 / 6,715 / + [in contig]
Location5444..6715 [in whole cluster]
5444..6715 [in contig]
TypeCDS
Length1,272 bp (423 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)AknB
Gene
Gene (GenBank)aknB
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • ketosynthase KSI; minimal PKS
Reference
ACC
PmId
[12137949] Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. (Gene. , 2002)
comment
aclacinomycin PKSを含んだ13.5kb領域のクローニング、シークエンス報告論文。

AknB: ketosynthase I (KS-alpha)
active siteを保存している。

plasmid pSgsPCR6(aknBCDE2F)は、S.galilaeusのminimal PKS不全mutant H071を相補し、anthracyclinone産生を回復。
S.lividans TK24 and A.coelicolor CH999において、pMC10aM導入でauramycinone産生。
pMC10aMのminimal PKS genes(snoa123)をaclacinomycin clusterのminimal PKS genes(aknBCDE2F)で置換して導入するとaklavinone産生。

aknB単独での機能確認はされていない。
Related Reference
ACC
Q55223
NITE
Dauno_00160
PmId
[9098068] Minimal Streptomyces sp. strain C5 daunorubicin polyketide biosynthesis genes required for aklanonic acid biosynthesis. (J Bacteriol. , 1997)
comment
Blast 13th, id69%, 1e-161
Streptomyces sp.(Strain C5)_dauA
Polyketide synthase

daunorubicin (DNR), doxorubicin (DXR)の前駆体であるaklanonic acidを異種性ホストで産生するために必要な最小遺伝子は、dpsG (ACP), dauI (regulatory activator), dpsA (KS alpha), dpsB (KS beta), dpsF (aromatase), dpsE (polyketide reductase), and dauG (putative deoxyaklanonic acid oxygenase)であるということを実証している。

dpsA(KS alpha)がこのentryに相当。

close this sectionPKS/NRPS Module

KS4..375

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [AknB]
MTARRVVITGLGVIAPGGIGTKAFWERVVSGVSATRTITAFDASGFRSRMAAECDFDGVR
SGLTVRDTARLDRATQFAVVAAREALADSGIEIDERNAHRTGVSLGSAVGCTQKLEEEYV
ARSDGGQRWLVDHAAGTPYLYDYFVPSSMAAEVAWEAGAEGPAALVSAGCTSGLDSLGHA
LDLIREGTVDIMIAGGSDAPIAPITVACFDAIKATSPRNDTPEHASRPFDRTRSGFVLGE
GAAVLVLEERESALRRGAQIYAEIAGYAGRGNAHHMTGLRPDGLEMSAAITGALDDARID
REAVGYVNAHGTATRQNDIHETAAIKHSLGEHAHRVPVSSIKAVIGHSLGAVGSIEAVAS
ALVIRHGVVPPTAGLHEPDPQLDLDYVPLTARDQATDTVLTVGSGFGGFQSAMVLTSAEG
SRS
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [AknB]
ATGACGGCCCGCCGGGTGGTGATCACCGGCCTGGGAGTGATCGCCCCGGGTGGCATCGGC
ACCAAGGCGTTCTGGGAACGCGTCGTCTCCGGCGTCTCCGCGACCAGGACCATCACGGCC
TTCGACGCGTCCGGGTTCCGCTCCCGGATGGCCGCGGAGTGCGACTTCGACGGCGTCCGC
TCGGGCCTGACCGTCCGTGACACCGCACGGCTGGACCGTGCCACCCAGTTCGCGGTGGTC
GCCGCGCGCGAGGCGCTCGCCGACAGCGGCATCGAGATCGACGAGAGGAACGCCCACCGC
ACCGGGGTCAGCCTCGGCTCCGCCGTGGGGTGCACCCAGAAGCTCGAGGAGGAGTACGTC
GCGCGCAGTGACGGCGGGCAGCGGTGGCTGGTCGATCACGCCGCCGGCACACCGTACCTG
TACGACTACTTCGTGCCGAGTTCGATGGCGGCCGAAGTGGCGTGGGAGGCCGGCGCCGAG
GGCCCCGCCGCGCTCGTCTCGGCGGGCTGCACCTCCGGTCTCGACTCCCTCGGGCACGCG
CTCGACCTCATCAGGGAGGGCACCGTCGACATCATGATCGCCGGTGGCAGCGACGCGCCC
ATCGCACCCATCACCGTGGCCTGTTTCGACGCCATCAAGGCGACCTCGCCGCGCAACGAC
ACCCCGGAGCACGCCTCGCGGCCCTTCGACAGGACCCGCAGCGGCTTCGTGCTCGGCGAG
GGTGCGGCCGTACTCGTCCTGGAGGAGCGGGAGTCCGCCCTGCGGCGCGGAGCACAGATC
TACGCCGAGATCGCGGGCTACGCCGGGCGTGGCAACGCCCACCACATGACCGGACTGCGC
CCCGACGGACTGGAGATGTCCGCCGCCATCACCGGCGCCCTCGACGACGCCCGCATCGAC
CGCGAAGCCGTCGGTTACGTCAACGCGCACGGTACGGCCACCCGGCAGAACGACATCCAC
GAGACGGCCGCGATCAAGCACAGCCTGGGCGAGCACGCCCACCGGGTCCCGGTCAGCTCC
ATCAAGGCGGTCATCGGTCACTCGCTCGGCGCCGTCGGCTCCATCGAGGCCGTCGCCTCC
GCCCTGGTGATCCGGCACGGAGTGGTGCCGCCCACGGCGGGACTGCACGAGCCCGATCCC
CAGCTCGACCTCGACTACGTTCCGCTGACCGCGCGCGATCAGGCCACCGACACCGTGCTG
ACGGTGGGCAGCGGATTCGGCGGATTCCAGAGCGCCATGGTGCTCACCAGCGCCGAAGGG
AGCCGCTCATGA
KS4..375
KS10..1125

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[4-252] 2.59999999999998e-58 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[261-375] 3.89999999999997e-33 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[2-266] 4.10000000000004e-64 G3DSA:3.40.47.10 [272-418] 1.09999999999999e-48 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[3-258] 2.89997807423187e-66 SSF53901 [220-419] 4.50001022902825e-61 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[161-177] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP
[1-20] 0.862 Signal
Eukaryota   
TMHMM No significant hit
Acla_00060 Acla_00060   Acla_00080 Acla_00080
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