Acla_00080 Acla_00080   Acla_00100 Acla_00100

Acla_00090 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction8,039 / 8,314 / + [in whole cluster]
8,039 / 8,314 / + [in contig]
Location8039..8314 [in whole cluster]
8039..8314 [in contig]
TypeCDS
Length276 bp (91 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)AknD
Gene
Gene (GenBank)aknD
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • acyl carrier protein
Reference
ACC
PmId
[12137949] Cloning and characterization of Streptomyces galilaeus aclacinomycins polyketide synthase (PKS) cluster. (Gene. , 2002)
comment
aclacinomycin PKSを含んだ13.5kb領域のクローニング、シークエンス報告論文。

AknD: Acyl carrier protein (ACP)

配列類似性からACPと判断。
overlapがあるので、たぶんaknE2と翻訳共役する。

plasmid pSgsPCR6(aknBCDE2F)は、S.galilaeusのminimal PKS不全mutant H071を相補し、anthracyclinone産生を回復。
S.lividans TK24 and A.coelicolor CH999において、pMC10aM導入でauramycinone産生。
pMC10aMのminimal PKS genes(snoa123)をaclacinomycin clusterのminimal PKS genes(aknBCDE2F)で置換して導入するとaklavinone産生。
Related Reference
ACC
Q9ZAU0
NITE
Adria_00040
PmId
[7828855] Characterization of the Streptomyces peucetius ATCC 29050 genes encoding doxorubicin polyketide synthase. (Gene. , 1994)
comment
Blast 17th, id44%, 1e-10
Streptomyces peucetius_dpsG
Daunorubicin acyl carrier protein

doxorubicin (DXR), daunorubicin (DNR)の中間体12-deoxyaklanonic acidの形成には、PKS領域遺伝子dpsF-dpsDと共にdpsGが発現される必要があることが報告されている。
ACC
P12885
NITE
Grana_00280
PmId
[8468280] Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins. (J Bacteriol. , 1993)
[9305965] Utilization of enzymatically phosphopantetheinylated acyl carrier proteins and acetyl-acyl carrier proteins by the actinorhodin polyketide synthase. (Biochemistry. , 1997)
comment
Blast 18th, id46%, 1e-10
Streptomyces violaceoruber_gra-orf3
Granaticin polyketide synthase acyl carrier protein

---
[PMID: 8468280](1993)
actinorhodin clusterのACPをgranaticin ACPで置換しても機能的なPKSが発現され、actVII mutantで見られるshunt産物である鎖状polyketide mutactinを産生する。

---
[PMID: 9305965](1997) abstract
E.coliのholo-ACP synthaseを使用して形成されたgranaticin holo-ACPは、actinorhodin PKSのKS-CLFと共に機能的であり、正規のactinorhodin PKSによって形成されるものと同じ芳香族polyketidesを合成する。

close this sectionPKS/NRPS Module

ACP5..77

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [AknD]
MSAFTVEELFQIMRECAGEEEAVDLADAAEQEFSLLGYDSLALMEAISRVERGLGIDLPE
ETVGEVLTPAAFVDVVNAELARSAPVVEAAG
selected fasta
>polyketide synthase acyl carrier protein subunit [AknD]
ATGTCCGCTTTCACGGTAGAAGAGCTTTTCCAGATCATGCGCGAGTGCGCGGGCGAGGAG
GAGGCCGTCGATCTGGCCGACGCCGCGGAGCAGGAGTTCTCCCTGCTCGGCTACGACTCC
CTCGCGCTCATGGAGGCGATCAGCAGGGTGGAGCGGGGTCTCGGCATCGACCTGCCCGAG
GAGACGGTGGGCGAGGTGCTGACGCCCGCCGCGTTCGTCGACGTCGTCAACGCCGAACTG
GCCCGCAGCGCGCCCGTGGTGGAGGCCGCCGGATGA
ACP5..77
ACP13..231

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
[35-50] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1-85] 2.2999993566538e-12 SSF47336
SSF47336   ACP_like
[5-81] 6.40000000000001e-13 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[5-77] PS50075
PS50075   ACP_DOMAIN
[25-75] 6.59999999999999e-07 PF00550
PF00550   PP-binding
SignalP
[1-28] 0.059 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Acla_00080 Acla_00080   Acla_00100 Acla_00100
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