Acla_00150 Acla_00150   Acla_00170 Acla_00170

Acla_00160 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction18,141 / 16,156 / - [in whole cluster]
1,986 / 1 / - [in contig]
Locationcomplement(16156..18141) [in whole cluster]
complement(1..1986) [in contig]
TypeCDS
Length1,986 bp (661 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.2 function unknown
Productbacteriocin biosynthesis cyclodehydratase domain protein(fragment)
Product (GenBank)AknN
Gene
Gene (GenBank)aknN
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11016846] A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin. (Mol Gen Genet. , 2000)
comment
anthracycline生合成gene clusterの15kb領域をクローニング、特徴づけ。
このclusterはaclacinomycinsの産生に関連するgeneを含む。

AknN: Unknown

クローニングされたclusterにtruncated aknN geneが含まれる。
機能がわかっているタンパクへの有意な類似は見られない。
機能不明なgranaticin pathway gene gra-ORF12に似ている。
Related Reference
ACC
Q52QI6
PmId
[15883371] Patellamide A and C biosynthesis by a microcin-like pathway in Prochloron didemni, the cyanobacterial symbiont of Lissoclinum patella. (Proc Natl Acad Sci U S A. , 2005)
comment
Blast 17th, id40%, 1e-100
Prochloron didemni_patD
Adenylation/heterocyclization protein

CyanobacteriumであるProchloron didemniの産生するLantibioticのクラスターを解析。

patDを含むクラスターを導入することでLantibioticを生産することを確認。
構造遺伝子とPeptidase以外にはpatD遺伝子しか含まれないため、このORFがAdenylationとHeterocyclizationを起こすと推測している。
PatDはN末にAdenylation活性、C末にHeterocyclization活性があるのではないかと推察。
Family/Domain
FD
IPR022291
comment
[IPR022291] Bacteriocin biosynthesis, cyclodehydratase (Domain)

close this sectionSequence

selected fasta
>bacteriocin biosynthesis cyclodehydratase domain protein(fragment) [AknN]
MTEDRVTTLGGEQIALLAPLLDGSRDLPGIVADAAPRLPAGLAERLVTRLLDAGLLCAYP
QDGADRPERAYRSLTGLQARSADARDAVLAAVDLTGDAESPLPEAVSAAGLRAAAPGEHA
ALTLVLCHDYLDPRLSALDAEHRATGRGWLPVRANGTHLWIGPFFSAGDGPCWSCLADRL
RLRRRGEAYVQHRLGHSGPAVHRRAYLPAGRAAALQLALLEAGKWLSGHRDTVQDSLWRL
DTRTLESSRHPVRRRPQCSRCGDPLLVRDRVSAPVVLSSRPVRDESGGGHRTFGPQEMLD
RYGHLVDPVTGVVGEIRRDPRGPEFLNCFTRSRCRLGPRAAPPALHSPLRSPGSGKGVTE
LHARVSALAEALERCSGYFQGDEPRRRGSYRELAGLAVHPDSVQLFDRRQFEDRRAWNRA
HGPFHQVTEPFDEDAPIDWTPVWSLTERRQRLAPTSLLYYNAPDADTGFCRATSNGAAAG
TSLEDAVVHGCLELVERDAIALWWYNRTRQPGVTLDARDPWITRLRAVLRDLGRTVWALD
LTSDLGIPVVAAVSVRTGGTAEDIVLGFGAHFDPRIALRRALTELSQMLPPLAQETAGDA
SAYTGTDPEAMRWFRHATTANQPYLLPAARRSARPPASLRPPRDAAAQAGALVALLRRHG
LE
selected fasta
>bacteriocin biosynthesis cyclodehydratase domain protein(fragment) [AknN]
ATGACCGAGGACCGGGTGACCACGCTGGGCGGGGAGCAGATCGCCCTGCTGGCCCCGCTG
CTCGACGGCAGCCGCGATCTGCCGGGCATCGTCGCCGACGCGGCACCCCGGCTGCCGGCC
GGACTCGCCGAGCGGCTCGTGACCCGGCTGCTGGACGCGGGACTGCTGTGCGCCTATCCC
CAGGACGGGGCCGACCGCCCCGAACGGGCCTACCGGTCCCTCACCGGTCTTCAGGCGCGG
TCCGCCGACGCGCGGGACGCGGTCCTGGCCGCGGTCGACCTCACCGGTGACGCGGAATCC
CCGCTGCCCGAGGCCGTGTCCGCGGCGGGGCTGCGCGCCGCGGCACCGGGCGAGCACGCC
GCTCTGACCCTGGTGCTCTGTCACGACTACCTCGACCCGCGGCTGAGTGCCCTGGACGCG
GAGCACCGCGCGACGGGACGCGGCTGGCTGCCCGTCCGGGCCAACGGCACCCACCTGTGG
ATCGGCCCGTTCTTCTCCGCCGGCGACGGCCCCTGCTGGAGCTGTCTGGCGGACCGGCTC
CGGCTGCGCCGGCGCGGTGAGGCGTACGTGCAGCACCGGCTGGGGCACAGCGGTCCGGCC
GTGCACCGGCGGGCCTACCTGCCCGCGGGGCGTGCCGCGGCCCTGCAACTGGCCCTGCTG
GAAGCCGGAAAGTGGCTCTCCGGACACCGCGACACGGTGCAGGACAGCCTCTGGCGCCTG
GACACCCGCACGCTGGAGAGCAGTCGCCATCCCGTGCGGCGCCGCCCGCAGTGCTCCCGC
TGCGGCGATCCGCTGCTGGTACGCGACCGGGTCTCGGCACCCGTGGTGCTGTCCTCCCGG
CCGGTGCGGGACGAGAGCGGCGGTGGTCACCGCACGTTCGGTCCGCAGGAGATGCTGGAC
CGCTACGGCCATCTCGTGGACCCCGTCACCGGCGTTGTCGGCGAGATCCGCCGGGATCCG
CGCGGACCGGAGTTCCTCAACTGCTTCACGCGGTCGCGCTGCCGTCTGGGACCCCGCGCT
GCACCGCCGGCGCTCCACTCCCCGCTGCGCAGCCCGGGATCCGGCAAGGGCGTCACCGAA
CTCCACGCCAGGGTCAGCGCGCTGGCCGAGGCGCTGGAGCGGTGCAGCGGCTACTTCCAG
GGCGACGAACCGCGCCGGCGCGGCAGTTACCGCGAGCTGGCCGGTCTCGCCGTGCATCCG
GACAGCGTCCAGCTCTTCGACCGCAGACAGTTCGAGGACCGGCGGGCGTGGAACCGCGCG
CACGGTCCCTTCCACCAGGTGACCGAGCCCTTCGACGAGGACGCCCCGATCGACTGGACC
CCCGTCTGGTCACTGACCGAGCGGCGGCAGCGGCTCGCGCCCACGAGTCTGCTCTACTAC
AACGCCCCGGACGCGGACACTGGCTTCTGCCGGGCCACGTCCAACGGAGCCGCCGCCGGA
ACGAGCCTGGAGGACGCCGTCGTGCACGGCTGCCTGGAACTCGTGGAACGGGACGCGATC
GCCCTGTGGTGGTACAACCGCACGCGCCAGCCCGGCGTGACCCTGGACGCGCGGGATCCG
TGGATCACGCGGCTCCGGGCCGTCCTGCGCGATCTGGGCAGGACGGTGTGGGCCCTCGAC
CTCACGTCCGATCTCGGCATTCCCGTGGTCGCCGCCGTGTCGGTGCGGACGGGCGGCACC
GCCGAGGACATCGTGCTGGGCTTCGGCGCGCACTTCGATCCGCGTATCGCCCTGCGCCGG
GCTCTCACCGAACTCAGCCAGATGCTCCCGCCGTTGGCGCAGGAGACGGCCGGCGACGCC
TCCGCGTACACCGGCACGGACCCCGAGGCCATGCGCTGGTTCCGGCACGCCACCACGGCA
AACCAGCCGTATCTCCTGCCCGCGGCGCGCCGGTCCGCCCGGCCCCCTGCCTCGCTGCGG
CCTCCCCGCGACGCCGCGGCACAGGCCGGGGCCCTCGTCGCCCTGCTGCGCCGGCACGGC
CTCGAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003776 YcaO-like (Domain)
[354-662] 4.30000000000003e-77 PF02624
PF02624   YcaO
[354-587] 1.4e-27 TIGR00702
TIGR00702   TIGR00702
IPR016040 NAD(P)-binding domain (Domain)
[125-262] 0.00021 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR022291 Bacteriocin biosynthesis, cyclodehydratase domain (Domain)
[148-313] 1.6e-44 TIGR03882
TIGR03882   Cyclo_dehyd_2
SignalP
[1-34] 0.184 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Acla_00150 Acla_00150   Acla_00170 Acla_00170
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