Acla_00140 Acla_00140   Acla_00160 Acla_00160

Acla_00150 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction13,513 / 15,150 / + [in whole cluster]
13,513 / 15,150 / + [in contig]
Location13513..15150 [in whole cluster]
13513..15150 [in contig]
TypeCDS
Length1,638 bp (545 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productaclacinomycin oxidoreductase
FAD-dependent oxidoreductase
Product (GenBank)aclacinomycin oxidoreductase
Gene
Gene (GenBank)aknOx
EC number
Keyword
  • cinerulose A
  • L-aculose
Note
Note (GenBank)
Reference
ACC
PmId
[17242508] Structure determination by multiwavelength anomalous diffraction of aclacinomycin oxidoreductase: indications of multidomain pseudomerohedral twinning. (Acta Crystallogr D Biol Crystallogr. , 2007)
[17395717] Aclacinomycin oxidoreductase (AknOx) from the biosynthetic pathway of the antibiotic aclacinomycin is an unusual flavoenzyme with a dual active site. (Proc Natl Acad Sci U S A. , 2007)
comment
[PMID: 17242508](2007) abstract
[PMID: 17395717](2007)

Aclacinomycin oxidoreductase (AknOx)は、Aclacinomycinグループのpolyketide抗生物質における最後の2steps、末端糖部分rhodinose→L-aculoseへの酸化を触媒する。

3つめの糖が
rhodinose (Aclacinomycin N)
↓1st: C-4 hydroxy基からプロトン除去[Tyr-450]
cinerulose A (Aclacinomycin A)
↓2nd: cineruloseのC3からプロトン除去[Tyr-378]
L-aculose (Aclacinomycin Y)

AknOxの異なる２つの反応が同じactive siteで触媒される。
この反応はFADの還元を伴う。

close this sectionSequence

selected fasta
>aclacinomycin oxidoreductase [aclacinomycin oxidoreductase]
MFVLNEFTRRGFLGTAAAVGGTTVVTTALGGAPAAQAAVPEAADGGACGARTALVKVDRV
DRRYQDLVTRGFNGRFRGRPDVVYVVHTADQVVDAVNQAMAAGQRIAVRSGGHCFEGFVD
DPAVRAVIDMSQMRQVFYDSGKRAFAVEPGATLGETYRALYLDWGVTIPAGVCPQVGVGG
HVLGGGYGPLSRRDGVVADHLYAVEVVVVDASGRARKVVATSAADDPNRELWWAHTGGGG
GNFGIVTRYWFRTPGATGTDPSQLLPKAPTSTLRHIVTWDWSALTEEAFTRIIDNHGAWH
QSNSAAGTPYASMHSVFYLNSRAAGQILLDIQIDGGLDGAEALLNDFVAAVNEGTGVEPA
VQRSTEPWLRATLANKFDTGGFDRTKSKGAYLRKPWTAAQAATLYRHLSADSQVWGEVSL
YSYGGKVNSVPETATATAQRDSIIKVWMSATWMDPAHDDANLAWIREIYREIFATTGGVP
VPDDRTEGTFINYPDVDLVDERWNTSGVPWYTLYYKGNYPRLQKVKARWDPRDVFRHALS
VRPPG
selected fasta
>aclacinomycin oxidoreductase [aclacinomycin oxidoreductase]
GTGTTTGTGTTGAACGAATTCACGCGCCGCGGATTCCTCGGCACCGCGGCAGCGGTCGGC
GGAACCACCGTGGTGACCACGGCCCTGGGCGGAGCCCCGGCCGCCCAGGCGGCCGTCCCG
GAAGCGGCGGACGGTGGCGCGTGCGGCGCGCGGACCGCGCTGGTGAAGGTGGACCGGGTG
GACCGGCGCTACCAGGACCTGGTCACCAGGGGCTTCAACGGGCGCTTCCGCGGCCGGCCC
GACGTCGTGTACGTCGTCCACACCGCGGACCAGGTCGTCGACGCGGTGAACCAGGCCATG
GCCGCGGGCCAGCGGATCGCGGTGCGCAGCGGCGGGCACTGCTTCGAGGGCTTCGTGGAC
GATCCCGCCGTCCGGGCCGTCATCGACATGTCCCAGATGAGGCAGGTCTTCTACGACTCC
GGCAAGCGGGCGTTCGCCGTGGAACCCGGCGCCACCCTCGGGGAGACGTACCGGGCCCTG
TATCTGGACTGGGGCGTGACCATCCCGGCCGGCGTCTGCCCCCAGGTGGGCGTCGGCGGC
CATGTGCTGGGCGGAGGCTACGGTCCGCTGTCCCGCCGGGACGGCGTGGTGGCCGACCAT
CTGTACGCGGTCGAGGTCGTCGTCGTGGACGCCTCGGGCCGGGCCCGCAAGGTCGTGGCC
ACGAGCGCCGCGGACGACCCCAACCGAGAGCTGTGGTGGGCCCACACCGGCGGCGGCGGA
GGCAACTTCGGGATCGTCACCCGGTACTGGTTCCGCACCCCCGGGGCCACCGGCACCGAC
CCGTCCCAACTCCTGCCCAAGGCCCCCACGTCCACCCTGCGCCACATCGTGACCTGGGAC
TGGTCCGCTCTCACCGAGGAGGCGTTCACCCGGATCATCGACAACCACGGGGCCTGGCAC
CAGAGCAACAGCGCCGCCGGCACCCCGTACGCCAGCATGCACAGCGTCTTCTACCTCAAC
AGCCGTGCGGCCGGTCAGATCCTGCTGGACATCCAGATCGACGGCGGCCTGGACGGCGCC
GAGGCCCTGCTGAACGACTTCGTGGCCGCGGTCAACGAGGGCACCGGCGTCGAGCCCGCC
GTGCAGCGGAGCACGGAGCCGTGGCTGCGCGCCACGCTGGCCAACAAGTTCGACACCGGC
GGCTTCGACCGGACCAAGTCGAAGGGCGCGTATCTGCGCAAGCCCTGGACCGCCGCCCAG
GCCGCCACTCTCTACCGCCATCTGAGCGCCGACTCCCAGGTCTGGGGAGAGGTCTCGCTC
TACTCGTACGGAGGCAAGGTCAACTCCGTGCCGGAGACGGCCACGGCCACGGCCCAGCGC
GACTCCATCATCAAGGTGTGGATGTCGGCGACCTGGATGGATCCCGCCCACGACGATGCC
AACCTCGCCTGGATCCGGGAGATCTACCGCGAGATCTTCGCCACCACCGGCGGCGTTCCG
GTGCCCGACGACCGCACGGAGGGCACCTTCATCAACTATCCCGACGTCGACCTGGTCGAC
GAGCGGTGGAACACCTCGGGAGTCCCCTGGTACACCCTCTACTACAAGGGGAACTACCCG
CGCCTGCAGAAGGTCAAGGCGCGCTGGGATCCCCGTGACGTCTTCCGGCACGCGCTGTCC
GTGCGGCCGCCCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006094 FAD linked oxidase, N-terminal (Domain)
[80-214] 1.6e-19 PF01565
PF01565   FAD_binding_4
IPR006311 Twin-arginine translocation pathway, signal sequence (Conserved_site)
[1-37] PS51318
PS51318   TAT
IPR012951 Berberine/berberine-like (Domain)
[490-542] 2.4e-17 PF08031
PF08031   BBE
IPR016166 FAD-binding, type 2 (Domain)
[61-257] 2.1000026783403e-36 SSF56176
SSF56176   FAD-binding_2
[76-256] PS51387
PS51387   FAD_PCMH
IPR016167 FAD-binding, type 2, subdomain 1 (Domain)
[72-134] 0.00027 G3DSA:3.30.43.10
G3DSA:3.30.43.10   FAD-binding_2_sub1
IPR016168 FAD-linked oxidase, FAD-binding, subdomain 2 (Domain)
[139-252] 7.90000000000001e-07 G3DSA:3.30.465.20
G3DSA:3.30.465.20   FAD-linked_oxidase_FAD-bd_sub2
SignalP
[1-37] 0.999 Signal
Bacteria, Gram-negative   
[1-37] 0.745 Signal
Eukaryota   
[1-37] 1 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Acla_00140 Acla_00140   Acla_00160 Acla_00160
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