Acla_00170 Acla_00170   Acla_00190 Acla_00190

Acla_00180 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction20,814 / 19,510 / - [in whole cluster]
4,659 / 3,355 / - [in contig]
Locationcomplement(19510..20814) [in whole cluster]
complement(3355..4659) [in contig]
TypeCDS
Length1,305 bp (434 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative pyridoxamine 5'-monophosphate-dependent NDP-hexose C-3 dehydratase
Product (GenBank)putative 3-dehydratase
Gene
Gene (GenBank)aknP
EC number
Keyword
  • L-rhodinose
Note
Note (GenBank)
  • AknP
Reference
ACC
PmId
[11016846] A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin. (Mol Gen Genet. , 2000)
[12427929] Characterization of mutations in aclacinomycin A-non-producing Streptomyces galilaeus strains with altered glycosylation patterns. (Microbiology. , 2002)
comment
[PMID: 11016846](2000)
anthracycline生合成gene clusterの15kb領域をクローニング、特徴づけ。
このclusterはaclacinomycinsの産生に関連するgeneを含む。

AknP: 3-Dehydratase

aknO, aknP and aknQを削除するとaclacinomycin非産生となることから、クローニングされたこのclusterがaclacinomycin生合成に関連すると確認している。
でもこのaclacinomycin生合成完全抑制は、おそらくaknOの不活化に由来すると述べられている。

AknPは3-dehydratasesに似ているが、それら遺伝子産物の機能はおおよそ推測である。
抗生物質生合成clustersにあるdehydratasesとは約75%の類似。

---
[PMID: 12427929](2002)
同じ著者らの続報。
上記論文を引用してFig. 2b(Sg9 gene cluster organization)とTable 4を記載。

Table 4. Putative functions of Sg9 gene products
AknP: dTDP-hexose 3-dehydratase

rhodinoseを合成できないmutants H075において、dTDP-hexose 3-dehydrataseをコードするaknPにナンセンス変異が見られる。
mutant H075の変異は、rdmI によってコードされるdTDP-hexose 3-dehydrataseによって相補される。
aknOPQRを含むplasmid pSgs5でも、mutant H075の変異は相補される。
Related Reference
ACC
Q9ALN8
NITE
Spino_00050
PmId
[17076492] Characterization of SpnQ from the spinosyn biosynthetic pathway of Saccharopolyspora spinosa: mechanistic and evolutionary implications for C-3 deoxygenation in deoxysugar biosynthesis. (J Am Chem Soc. , 2006)
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
Blast 14th, id72%, 1e-177
Saccharopolyspora spinosa_spnQ
probable NDP-hexose-3,4-dehydratase
[DoBISCUIT]pyridoxamine 5'-monophosphate-dependent NDP-hexose C-3 dehydratase

--
[PMID: 17076492](2006)
SpnQをクローニング、過剰発現、精製して、"ColD-like" conditions と "E1-like" conditionsの両方で触媒活性をテスト。
E3のような特異的reductaseを必要とせず、一般的なferredoxin/ferredoxin reductase またはflavodoxin/flavodoxin reductaseと効率的に働く。

--
[PMID: 18345667](2008)
dTDP-D-forosamine形成に関連する5つの酵素SpnO, N, Q, R, and Sの生化学的機能解明。
使用している株はNRRL 18537.
SpnQのsteady-state kinetic parameters、基質特異性を調査。

close this sectionSequence

selected fasta
>putative pyridoxamine 5'-monophosphate-dependent NDP-hexose C-3 dehydratase [putative 3-dehydratase]
MTSDTKALVLEQVREYHRQQQPGNFQPGVTPILSSGAVLDEEDRVALVEAALDLRIAAGA
HSRRFESKFARHIGVRKAHLVNSGSSANLLALSALTSPRLGEQRLRPGDEVITVAGGFPT
TVNPILQNGLTPVFVDLELGTYNTTVEHVRAAISDRTRAIMIAHTLGNPYQVAEIQQLAT
EHELFLIEDNCDAVGSTYQGRMTGTFGDLATVSFYPAHHITTGEGGCVLTRNLELARIVE
SFRDWGRDCWCEPGEDNTCLKRFDYQLGNLPKGYDHKYIFSHIGYNLKATDLQGALALSQ
LNKLPEFGAARRRNWQRLRDGLADVPGLLLPVATPGSDPSWFGFVITVLPDATYTRRDLV
AFLEERRIGTRRLFGGNLTRHPAYLGTPHRVAGDLRNSDIITEQSFWIGVYPGITEEMTD
YMRESIVEFVTKNG
selected fasta
>putative pyridoxamine 5'-monophosphate-dependent NDP-hexose C-3 dehydratase [putative 3-dehydratase]
ATGACGTCGGACACCAAGGCTCTGGTCCTGGAGCAGGTTCGCGAGTATCACCGGCAGCAG
CAGCCAGGGAATTTCCAGCCCGGAGTGACGCCCATCCTCTCCTCGGGCGCCGTCCTGGAC
GAGGAGGACCGGGTCGCCCTGGTGGAGGCCGCCCTGGATCTGCGGATCGCGGCCGGAGCC
CACTCCCGGCGCTTCGAGAGCAAATTCGCCCGCCACATCGGCGTACGCAAGGCGCACCTG
GTGAACTCGGGGTCCTCGGCGAACCTGCTGGCGCTCTCTGCGCTGACCTCTCCCCGGCTC
GGTGAGCAGCGGCTGCGTCCGGGTGACGAGGTGATCACGGTGGCCGGCGGATTCCCCACC
ACGGTGAACCCGATCCTCCAGAACGGGCTGACGCCCGTGTTCGTGGACCTGGAACTCGGT
ACGTACAACACAACGGTGGAGCACGTGCGGGCGGCGATCTCGGACCGGACCCGGGCCATC
ATGATCGCGCACACCCTCGGCAACCCCTACCAGGTGGCGGAGATCCAGCAACTGGCCACC
GAGCACGAGCTGTTCCTGATCGAGGACAACTGCGACGCGGTGGGCTCGACCTACCAGGGA
CGTATGACGGGGACCTTCGGGGACCTGGCAACGGTCAGTTTCTATCCTGCCCACCACATC
ACCACGGGCGAGGGGGGCTGCGTACTGACCCGCAACCTCGAACTGGCCCGCATCGTCGAG
TCGTTCCGCGACTGGGGCCGGGACTGCTGGTGCGAACCGGGCGAGGACAACACCTGCCTC
AAGCGGTTCGACTACCAGCTGGGTAATCTCCCCAAGGGCTACGACCACAAGTACATCTTC
TCCCACATCGGTTACAACCTGAAGGCGACCGATCTGCAAGGGGCGCTGGCACTCAGCCAG
TTGAACAAGCTGCCGGAGTTCGGCGCGGCCCGGCGGCGCAACTGGCAGCGGCTGCGGGAC
GGGCTCGCCGACGTCCCCGGTCTGCTGCTGCCGGTCGCGACACCGGGCAGCGATCCCAGC
TGGTTCGGGTTCGTGATCACGGTCCTGCCCGACGCCACCTACACCCGCCGTGACCTGGTC
GCCTTCCTGGAGGAACGGCGGATCGGCACACGGCGGCTGTTCGGCGGCAACCTCACCCGC
CATCCGGCGTACCTCGGCACGCCGCACCGGGTGGCGGGCGATCTGCGCAACTCCGACATC
ATCACCGAGCAGAGCTTCTGGATCGGGGTGTATCCGGGCATCACGGAGGAGATGACCGAC
TACATGCGGGAGTCCATCGTCGAGTTCGTCACCAAGAACGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[1-434] 8.79996401030984e-117 PIRSF000390
PIRSF000390   PLP_StrS
[41-426] 1.69999999999998e-100 PF01041
PF01041   DegT_DnrJ_EryC1
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[32-302] 1.9e-71 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[303-426] 1.09999999999999e-47 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[26-433] 3.19999899046355e-93 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
Acla_00170 Acla_00170   Acla_00190 Acla_00190
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