Acla_00270 Acla_00270   last last

Acla_00280 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction29,939 / 30,962 / + [in whole cluster]
13,784 / 14,807 / + [in contig]
Location29939..30962 [in whole cluster]
13784..14807 [in contig]
TypeCDS
Length1,024 bp (340 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 aminotransferase(fragment)
Product (GenBank)putative aminotransferase
Gene
Gene (GenBank)aknZ
EC number
Keyword
  • L-rhodosamine
Note
Note (GenBank)
  • AknZ
Reference
ACC
PmId
[11016846] A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin. (Mol Gen Genet. , 2000)
[12427929] Characterization of mutations in aclacinomycin A-non-producing Streptomyces galilaeus strains with altered glycosylation patterns. (Microbiology. , 2002)
comment
[PMID: 11016846](2000)
anthracycline生合成gene clusterの15kb領域をクローニング、特徴づけ。

AknZ: Aminotransferase

incomplete ORF.
AknX, Y, Zは糖残基の生合成に関連付けられる。

glycosyltransferase (aknS), aminomethylase (aknX), glucose-1-phosphate thymidylyltransferase (aknY) とunidentified glycosylation functions (aknT and aknV)を含むplasmid pSgs4は、aklavinoneを蓄積するS. galilaeus mutants H063と、rhodinose and deoxyfucose residuesを持つaklavinoneを産生するH054でaclacinomycinsの産生を回復。

ε-rhodomycinone(RHO)を蓄積するS. peucetius mutants M18におけるpSgs4の導入は、
RHO, L-rhamnosyl-RHO, L-daunosaminyl-RHO をもたらす。
glycosyltransferasesに欠損がないRHO蓄積S. peucetius mutants M90へのpSgs4の導入は、
RHO と L-rhamnosyl-RHO をもたらす。

---
[PMID: 12427929](2002)
同じ著者らの続報。
上記論文を引用してFig. 2b(Sg9 gene cluster organization)とTable 4を記載。

Table 4. Putative functions of Sg9 gene products
AknZ: aminotransgerase(>340aa)

H054とneutral glycosidesを持つaklavinoseを産生するH065 mutantは、pSgs44(aknW, X2, Y, C末30aaを欠いたaknZ)、pSgs45(aknY, C末30aaを欠いたaknZ)である程度相補される。
mutantの表現型はAknX2に由来しないことを確認しており、AknZのC末30aa不足はaminotransferase活性を弱めるが廃止まではしないので、ある程度の相補に至っている、とのこと。
Related Reference
ACC
Q9F837
NITE
Megalo_00050
PmId
[16514147] In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea. (Microbiology. , 2006)
[20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010)
comment
Blast 19th, id74%, 1e-143
Micromonospora megalomicea subsp. nigra_megDII
TDP-3-keto-6-deoxyhexose 3-aminotransaminase
[DoBISCUIT]NDP-hexose C-3 aminotransferase

異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS解析。セットの組み合わせによりMegDII の機能は確立されている。

MegDII は、desosamineとmegosamine両方の生合成において3-aminotransferaseとして働く。
基質は各経路で少し異なる。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 aminotransferase(fragment) [putative aminotransferase]
MTTLVWDYLQEYENERADILDAVETVFSSGRLVLGDSVRGFEEEFAAYHGAAHCVGVDNG
TNAIKLALQALGVGPGDEVVTVSNTAAPTVVAIDSVGATPVFVDVHPDSYLMDTEQVEAA
LTPRTRCLLPVHLYGQCVDLAPLERLAAEHDLFLVEDCAQAHGARRAGRLAGTTGDAAAF
SFYPTKVLGAYGDGGAVVTSRDDTHRALRRLRYYGMEERYYVVGTPGHNARLDEVQAEIL
RRKLRRLDTYIEGRRAVARRYEDGLGDTGLVLPHTVPGNEHVYYVYTVRHPRRDDIIKAL
KAYDIELNISYPWPVHTMSGFAHLGYGKGSLPVTEDLAGQI-
selected fasta
>putative NDP-hexose C-3 aminotransferase(fragment) [putative aminotransferase]
ATGACGACTCTCGTCTGGGACTACCTACAGGAATACGAGAACGAACGCGCCGACATTCTG
GACGCCGTGGAGACGGTCTTCAGCTCGGGTCGGCTCGTCCTCGGTGACAGCGTTCGCGGA
TTCGAGGAGGAGTTCGCCGCGTACCACGGCGCGGCGCACTGCGTCGGTGTCGACAACGGC
ACCAACGCGATCAAGCTGGCCCTCCAGGCGCTCGGCGTCGGCCCCGGTGACGAGGTCGTC
ACGGTGTCCAACACCGCGGCCCCCACGGTCGTGGCCATCGATTCGGTGGGCGCCACCCCG
GTGTTCGTCGACGTCCACCCGGACAGCTACCTCATGGACACCGAGCAGGTGGAGGCCGCA
CTCACGCCCCGGACCCGATGCCTGCTGCCGGTCCATCTCTACGGGCAGTGCGTCGACCTG
GCTCCGCTGGAGCGGCTCGCCGCGGAGCACGACCTGTTCCTCGTCGAGGACTGCGCACAG
GCCCACGGTGCCCGCCGCGCCGGCCGGCTCGCCGGCACCACCGGCGACGCCGCCGCCTTC
TCCTTCTACCCCACGAAGGTGCTCGGCGCCTACGGCGACGGAGGCGCCGTGGTGACCTCC
CGGGACGACACCCACCGCGCGCTGCGCCGACTGCGCTACTACGGCATGGAGGAGCGGTAC
TACGTCGTCGGCACCCCGGGTCACAACGCCCGGCTCGACGAGGTCCAGGCCGAGATCCTG
CGGCGCAAGCTGCGCCGGCTCGACACCTACATCGAGGGGCGGCGGGCCGTCGCCCGGCGC
TACGAGGACGGGCTCGGCGACACCGGCCTGGTGCTCCCGCACACCGTCCCCGGCAACGAG
CACGTGTACTACGTCTACACGGTGCGCCACCCGCGGCGCGACGACATCATCAAGGCCCTC
AAGGCGTACGACATCGAGCTGAACATCAGCTATCCCTGGCCCGTGCACACCATGTCCGGG
TTCGCCCACCTCGGCTACGGCAAGGGCTCGCTGCCCGTCACCGAGGACCTGGCCGGCCAG
ATCT

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[15-340] 1.6e-110 PF01041
PF01041   DegT_DnrJ_EryC1
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[16-246] 9.5e-77 G3DSA:3.40.640.10
G3DSA:3.40.640.10   no description
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[247-341] 2.5e-23 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   no description
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[16-341] 2.7e-96 SSF53383
SSF53383   PLP-dependent transferases
SignalP No significant hit
TMHMM No significant hit
Acla_00270 Acla_00270   last last
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