Acla_00260 Acla_00260   Acla_00280 Acla_00280

Acla_00270 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction29,716 / 28,841 / - [in whole cluster]
13,561 / 12,686 / - [in contig]
Locationcomplement(28841..29716) [in whole cluster]
complement(12686..13561) [in contig]
TypeCDS
Length876 bp (291 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative glucose-1-phosphate thymidylyltransferase
putative dTDP-glucose synthase
Product (GenBank)putative dTDP-1-glucose synthase
Gene
Gene (GenBank)aknY
EC number2.7.7.24
Keyword
  • L-rhodosamine
  • L-2-deoxyfucose
  • L-rhodinose
Note
Note (GenBank)
  • AknY
Reference
ACC
PmId
[11016846] A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin. (Mol Gen Genet. , 2000)
[12427929] Characterization of mutations in aclacinomycin A-non-producing Streptomyces galilaeus strains with altered glycosylation patterns. (Microbiology. , 2002)
comment
[PMID: 11016846](2000)
anthracycline生合成gene clusterの15kb領域をクローニング、特徴づけ。

AknY: Glucose-1-phosphate thymidylyltransferas

AknX, Y, Zは糖残基の生合成に関連付けられる。

glycosyltransferase (aknS), aminomethylase (aknX), glucose-1-phosphate thymidylyltransferase (aknY) とunidentified glycosylation functions (aknT and aknV)を含むplasmid pSgs4は、aklavinoneを蓄積するS. galilaeus mutants H063と、rhodinose and deoxyfucose residuesを持つaklavinoneを産生するH054でaclacinomycinsの産生を回復。

ε-rhodomycinone(RHO)を蓄積するS. peucetius mutants M18におけるpSgs4の導入は、
RHO, L-rhamnosyl-RHO, L-daunosaminyl-RHO をもたらす。
glycosyltransferasesに欠損がないRHO蓄積S. peucetius mutants M90へのpSgs4の導入は、
RHO と L-rhamnosyl-RHO をもたらす。

また、aknW, aknX2, aknYとC末を欠いたaknZを含むpSgs44は、H054とH065 mutantを部分的に相補する。

---
[PMID: 12427929](2002)
同じ著者らの続報。
上記論文を引用してFig. 2b(Sg9 gene cluster organization)とTable 4を記載。

Table 4. Putative functions of Sg9 gene products
AknY: dTDP-glucose 1-synthase

糖部分を持たないaklavinoneを蓄積するmutatn H063では、aknYによってコードされるdTDP-glucose synthaseにAA置換が起こっている。
mutant H063でPCRで増幅したaknYを発現させると変異は相補された。
Related Reference
ACC
Q9AGY4
PmId
[12147463] Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155. (Appl Environ Microbiol. , 2002)
comment
Blast 43rd, id59%, 4e-98
Aneurinibacillus thermoaerophilus_rmlA
Glucose-1-phosphate thymidylyltransferase(EC 2.7.7.24)

Aneurinibacillus thermoaerophilusにおいて、Glucose-1-phosphate thymidylyltransferase (rmlA)を
E. coliで発現させ、活性等の諸性質について調べている。

close this sectionSequence

selected fasta
>putative glucose-1-phosphate thymidylyltransferase [putative dTDP-1-glucose synthase]
MKGIILAGGSGTRLHPITVSVSKQLLPVGDKPMIYYPLSVLMLADIREILLICTERDLEQ
FRRLLGDGSQLGLRIDYAVQNRPAGLADAFVIGADHVGDDDVALVLGDNIFHGHHFYDLL
QSNVHDVQGCVLFGYPVEDPERYGVGETDASGQLVSLEEKPLRPRSDLAITGLYLYDNEV
VDIAKNLRPSPRGELEITDVNRNYLARGRARLVDLGRGFAWLDAGTPESLLQATQYVRTL
EERQGVRIACVEEVALRMGFIDADMCHRLGEQMSQSGYGRYVMAVAREFSG
selected fasta
>putative glucose-1-phosphate thymidylyltransferase [putative dTDP-1-glucose synthase]
ATGAAGGGGATTATTCTCGCCGGCGGTTCGGGAACACGGCTCCATCCGATAACCGTCTCC
GTATCAAAACAGCTTCTTCCGGTCGGCGACAAGCCGATGATCTACTATCCGCTCTCGGTG
CTGATGCTGGCGGACATCAGGGAGATCCTGCTCATCTGCACCGAGCGCGATCTGGAACAG
TTCCGCCGGCTCCTGGGCGACGGCTCCCAGCTCGGCCTGCGCATCGACTACGCGGTGCAG
AACCGGCCGGCCGGACTGGCCGACGCCTTCGTCATCGGCGCCGACCACGTGGGTGACGAC
GACGTGGCACTGGTCCTCGGGGACAACATCTTCCACGGACACCACTTCTACGACCTGCTC
CAGAGCAACGTCCACGACGTCCAGGGCTGCGTGCTGTTCGGCTACCCCGTGGAGGACCCG
GAGCGCTACGGCGTGGGCGAGACCGACGCGTCGGGGCAACTCGTCTCCCTGGAGGAGAAG
CCGCTCCGGCCGCGTTCCGACCTCGCCATCACCGGGCTCTATCTGTACGACAACGAAGTG
GTGGACATCGCGAAGAACCTCCGGCCCTCGCCGCGCGGCGAACTGGAGATCACCGATGTG
AACCGGAATTACCTGGCCCGCGGCCGAGCGCGTCTGGTCGATCTCGGCCGCGGTTTCGCC
TGGCTCGACGCCGGAACCCCGGAATCGCTGCTTCAGGCCACCCAGTACGTGCGCACGCTC
GAAGAACGGCAGGGCGTGCGCATCGCCTGCGTCGAAGAAGTGGCGCTGCGCATGGGATTC
ATCGACGCGGACATGTGCCACCGACTCGGTGAGCAGATGTCGCAGTCCGGTTACGGTCGC
TATGTGATGGCGGTCGCCCGAGAATTCTCGGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005835 Nucleotidyl transferase (Domain)
[2-237] 3.40000000000004e-63 PF00483
PF00483   NTP_transferase
IPR005907 Glucose-1-phosphate thymidylyltransferase, short form (Family)
[2-286] 1.39999999999997e-128 TIGR01207
TIGR01207   RmlA
SignalP No significant hit
TMHMM No significant hit
Acla_00260 Acla_00260   Acla_00280 Acla_00280
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