Acla_00250 Acla_00250   Acla_00270 Acla_00270

Acla_00260 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction28,112 / 28,828 / + [in whole cluster]
11,957 / 12,673 / + [in contig]
Location28112..28828 [in whole cluster]
11957..12673 [in contig]
TypeCDS
Length717 bp (238 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose N,N-dimethyltransferase
Product (GenBank)putative aminomethylase
Gene
Gene (GenBank)aknX2
EC number2.1.1.-
Keyword
  • L-rhodosamine
Note
Note (GenBank)
  • AknX2
Reference
ACC
PmId
[11016846] A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin. (Mol Gen Genet. , 2000)
[12427929] Characterization of mutations in aclacinomycin A-non-producing Streptomyces galilaeus strains with altered glycosylation patterns. (Microbiology. , 2002)
comment
[PMID: 11016846](2000)
anthracycline生合成gene clusterの15kb領域をクローニング、特徴づけ。

AknX: Aminomethylase

AknXはN-methyltransferasesに約60%の類似を示す。
AknX, Y, Zは糖残基の生合成に関連付けられる。

glycosyltransferase (aknS), aminomethylase (aknX), glucose-1-phosphate thymidylyltransferase (aknY) とunidentified glycosylation functions (aknT and aknV)を含むplasmid pSgs4は、aklavinoneを蓄積するS. galilaeus mutants H063と、rhodinose and deoxyfucose residuesを持つaklavinoneを産生するH054でaclacinomycinsの産生を回復。

ε-rhodomycinone(RHO)を蓄積するS. peucetius mutants M18におけるpSgs4の導入は、
RHO, L-rhamnosyl-RHO, L-daunosaminyl-RHO をもたらす。
glycosyltransferasesに欠損がないRHO蓄積S. peucetius mutants M90へのpSgs4の導入は、
RHO と L-rhamnosyl-RHO をもたらす。

また、aknW, aknX2, aknYとC末を欠いたaknZを含むpSgs44は、H054とH065 mutantを部分的に相補する。

--
[PMID: 12427929](2002)
同じ著者らの続報。
上記論文を引用してFig. 2b(Sg9 gene cluster organization)とTable 4を記載。

Table 4. Putative functions of Sg9 gene products
AknX2: aminomethylase
よってここでのAknX2=AknX

上記論文の結果を受けて行った実験で、mutantの表現型はAknX2に由来しないことを確認している。
Related Reference
ACC
Q9ZGH6
NITE
Pikro_00100
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[12119032] Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine. (Biochemistry. , 2002)
comment
Blast 8th, id55%, 2e-70
Streptomyces venezuelae_desVI
N,N-dimethyltransferase

---
[PMID: 9770448](1998)
12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。

DesVI: N,N-Dimethyltransferase

desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。

desVI のDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。それらのaglyconeである10-deoxymethynolide and narbonolideを蓄積。

---
[PMID: 12119032](2002)abstract
N,N-dimethyltransferasesであるtylM1 and desVI の過剰発現、精製、触媒機能確認。
TylM1 and DesVI はhomodimeric proteinで、ほとんど同じ生化学的特性を持つ。

close this sectionSequence

selected fasta
>putative NDP-hexose N,N-dimethyltransferase [putative aminomethylase]
MYGRELADVYEAIYRSRGKDWGQEAADVSRIITERRPGAGSLLDVACGTGAHLSVFSTLF
EVAEGLEIAEPMRRLAEQRLPGTTVHAGDMRDFRLPRTYDAVSCMFCAIGYLETLDDMRA
AVRSMAAHLEPGGVLVVEPWWFPENFIEGYVAGDLAREEHRTIARISHTTRKGRATRMEV
RFTVGDAAGIQQFTEIDVLTLFTRDEYTAAFTDAGCSVEFLEDGPTGRGLFVGVREQR
selected fasta
>putative NDP-hexose N,N-dimethyltransferase [putative aminomethylase]
ATGTACGGCCGGGAACTCGCGGACGTGTACGAGGCCATCTACCGCAGCCGCGGCAAGGAC
TGGGGACAGGAGGCGGCGGACGTCTCGCGGATCATCACCGAACGGCGTCCGGGAGCCGGC
TCGCTGCTCGACGTCGCCTGTGGCACGGGCGCCCATCTGAGCGTGTTCAGCACGCTGTTC
GAGGTCGCCGAGGGCCTGGAGATCGCGGAGCCGATGCGGCGGCTCGCCGAGCAGCGGCTG
CCCGGCACCACCGTGCACGCCGGCGACATGCGCGACTTCCGGCTCCCGCGCACCTACGAC
GCGGTGAGCTGCATGTTCTGCGCCATCGGCTATCTGGAGACGCTGGACGACATGCGGGCC
GCCGTCCGGTCGATGGCCGCTCATCTGGAGCCCGGCGGCGTGCTGGTCGTCGAACCCTGG
TGGTTCCCCGAGAACTTCATCGAGGGCTATGTCGCGGGTGACCTGGCCCGCGAGGAGCAC
CGGACCATCGCCCGGATCTCGCACACCACCCGGAAGGGCCGGGCCACCCGCATGGAGGTT
CGCTTCACCGTGGGGGACGCCGCCGGCATCCAGCAGTTCACGGAGATCGACGTGCTGACC
CTGTTCACCAGGGACGAATACACCGCCGCGTTCACCGACGCCGGCTGTTCCGTTGAATTC
CTGGAGGACGGACCCACCGGCCGCGGTCTTTTCGTCGGTGTTCGCGAACAGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Acla_00250 Acla_00250   Acla_00270 Acla_00270
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