first first   Alnu_00020 Alnu_00020

Alnu_00010 : CDS information

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Organism
StrainCM020
Entry nameAlnumycin
Contig
Start / Stop / Direction7 / 513 / + [in whole cluster]
7 / 513 / + [in contig]
Location7..513 [in whole cluster]
7..513 [in contig]
TypeCDS
Length507 bp (168 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative MarR family transcriptional regulator
Product (GenBank)AlnR1
Gene
Gene (GenBank)alnR1
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[18940666] Characterization of the alnumycin gene cluster reveals unusual gene products for pyran ring formation and dioxan biosynthesis. (Chem Biol. , 2008)
comment
alnumycin gene clusterのクローニング、シークエンス。
aln2-alnT2の領域をS.albusに導入して、alnumycin産生されることを確認している。
この結果からalnR1 and aln1はalnumycin生合成に絶対的に必要ではないが、それでもnative hostでの関与は除外できない、と述べられている。

AlnR1: MarR family regulator
配列解析からMarR familyのregulatorであると提唱されている。
Family/Domain
FD
IPR000835
comment
[IPR000835] HTH transcriptional regulator, MarR (Family)
function; sequence-specific DNA binding transcription factor activity

close this sectionSequence

selected fasta
>putative MarR family transcriptional regulator [AlnR1]
MADLEVEAMSEEPEAEAGSRLTAEEAVRAMLVTLPRLVSRAKRTPVPEQLRSLRLAPRHL
SLLSCLIFDGPTSVKDLAARLEVAPTTVSLMVSDLQREGVVERRSDPDDRRRSIVSLTED
PTTRAAIDAWLASGARAWRKVFDDLSPQERATFVRAIQRYEEAVAEAP
selected fasta
>putative MarR family transcriptional regulator [AlnR1]
GTGGCGGACCTGGAGGTCGAAGCGATGTCAGAGGAGCCGGAGGCGGAGGCGGGCAGTCGT
CTCACCGCCGAAGAGGCGGTCCGGGCGATGCTGGTCACCCTGCCTCGGCTGGTCTCCCGT
GCCAAGCGCACCCCCGTGCCCGAGCAGTTGCGCTCGTTGCGGCTGGCGCCCCGGCACCTC
TCCCTGCTGTCCTGTCTCATCTTCGACGGGCCCACGTCCGTCAAGGACCTCGCCGCTCGG
CTGGAGGTCGCGCCGACCACGGTCAGTCTGATGGTCAGCGATCTGCAGCGCGAGGGTGTT
GTGGAGCGCCGGTCCGATCCCGACGACCGCAGGCGCAGCATCGTCTCGCTGACCGAGGAT
CCCACCACCCGCGCGGCCATTGACGCCTGGCTGGCCAGTGGGGCCAGGGCCTGGCGCAAG
GTCTTCGACGACCTCAGTCCGCAGGAGCGCGCGACCTTCGTACGGGCCATCCAGCGTTAC
GAGGAAGCCGTCGCCGAAGCACCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000835 Transcription regulator HTH, MarR-type (Family)
[28-162] PS50995
PS50995   HTH_MARR_2
[48-150] 3.0999994589937e-14 SM00347
SM00347   HTH_MARR
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[36-167] 5.40000000000002e-25 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
SignalP No significant hit
TMHMM No significant hit
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