Alnu_00160 Alnu_00160   Alnu_00180 Alnu_00180

Alnu_00170 : CDS information

close this sectionLocation

Organism
StrainCM020
Entry nameAlnumycin
Contig
Start / Stop / Direction14,040 / 13,510 / - [in whole cluster]
14,040 / 13,510 / - [in contig]
Locationcomplement(13510..14040) [in whole cluster]
complement(13510..14040) [in contig]
TypeCDS
Length531 bp (176 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.3 uncharacterized protein (biosynthesis involved)
Producthypothetical protein
Product (GenBank)Aln5
Gene
Gene (GenBank)aln5
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[18940666] Characterization of the alnumycin gene cluster reveals unusual gene products for pyran ring formation and dioxan biosynthesis. (Chem Biol. , 2008)
comment
alnumycin gene clusterのクローニング、シークエンス。
aln2-alnT2の領域をS.albusに導入して、alnumycin産生されることを確認している。

Aln5: Involved in pyran ring formation

aln4 or aln5の不活化mutantはalnumycin産生をなくし、唯一の産物としてDMACに似たshunt産物K1115Aを産生する。
Aln5はalnumycin pathwayにおいて、reductase Aln4のaccessory proteinとして機能するかもしれない。

--
>補足
[PMID: 22474343](2012)において、Aln4はpyran環形成ではなく、dioxane部分の形成に関与することがわかっている。このdioxane部分の形成にAln5は必要とされない。AlnA, AlnB, Aln6, Aln4が関与する。

close this sectionSequence

selected fasta
>hypothetical protein [Aln5]
MTPEEVVGVWRLASYTEVGEDGGTVAGPLGEAPAGLLIYTADGHVAVSMMKTGDAPALET
YMGYSGQWRLAGDRMTHRVQVSAHPRMAGTEQIRRVALDGETLSLRGTAVTPVGGRAPER
VLTWRRAKPEGIAADGTASGSTAFDGTGSGGTAFDSTAFDSTASDETAEQQEKETH
selected fasta
>hypothetical protein [Aln5]
GTGACGCCGGAGGAGGTCGTCGGAGTGTGGCGGCTGGCCTCGTACACGGAGGTGGGCGAG
GACGGGGGCACGGTGGCGGGGCCGTTGGGCGAGGCGCCGGCCGGGCTGCTGATCTACACC
GCGGACGGGCATGTCGCGGTGAGCATGATGAAGACCGGGGACGCCCCGGCCCTGGAGACC
TACATGGGCTACTCGGGGCAGTGGCGGCTGGCCGGGGACCGGATGACGCACCGGGTCCAG
GTCAGCGCCCATCCGCGGATGGCGGGCACCGAGCAGATACGCCGCGTCGCGCTCGACGGC
GAAACGCTGTCGCTGCGCGGCACGGCCGTGACACCGGTGGGGGGCCGCGCGCCGGAACGC
GTACTCACCTGGCGGCGCGCGAAGCCGGAAGGCATCGCGGCCGACGGCACGGCATCCGGC
AGCACGGCGTTCGACGGCACGGGATCCGGCGGCACGGCGTTCGACAGCACAGCTTTCGAC
AGCACGGCGTCCGACGAGACGGCGGAACAGCAGGAGAAGGAGACGCACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR024311 Lipocalin-like domain (Domain)
[7-127] 6.1e-31 PF13924
PF13924   Lipocalin_5
SignalP No significant hit
TMHMM No significant hit
Alnu_00160 Alnu_00160   Alnu_00180 Alnu_00180
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