Alnu_00210 Alnu_00210   Alnu_00230 Alnu_00230

Alnu_00220 : CDS information

close this sectionLocation

Organism
StrainCM020
Entry nameAlnumycin
Contig
Start / Stop / Direction19,009 / 18,194 / - [in whole cluster]
19,009 / 18,194 / - [in contig]
Locationcomplement(18194..19009) [in whole cluster]
complement(18194..19009) [in contig]
TypeCDS
Length816 bp (271 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative transcriptional regulator
Product (GenBank)AlnR3 SARP family regulator
Gene
Gene (GenBank)alnR3
EC number
Keyword
  • SARP family
Note
Note (GenBank)
Reference
ACC
PmId
[18940666] Characterization of the alnumycin gene cluster reveals unusual gene products for pyran ring formation and dioxan biosynthesis. (Chem Biol. , 2008)
comment
alnumycin gene clusterのクローニング、シークエンス。
aln2-alnT2の領域をS.albusに導入して、alnumycin産生されることを確認している。

AlnR3: SARP family regulator

SARP familyのpathway-specific antibiotic biosynthesis activatorsと配列類似あり。
Related Reference
ACC
Q9XCC4
PmId
[11985721] Differential roles of two SARP-encoding regulatory genes during tylosin biosynthesis. (Mol Microbiol. , 2002)
[16942604] Regulation of tylosin biosynthesis involving 'SARP-helper' activity. (Mol Microbiol. , 2006)
comment
Blast 17th, id56%, 5e-64
Streptomyces fradiae_tylS
Hypothetical pathway specific regulatory protein TylS

---
[PMID:11985721](2002)
tylSはtylosin産生に必須。
TysSはtylRなどの遺伝子発現を調節する。

--
[PMID:16942604](2006)
TylSでtylUは調節される。
tylU破壊でTylRが減少し、tylosin収量が80%になる。
TylS and TylUは、tylRの発現を促進するために協調して働く。
ACC
P25047
NITE
Adria_00210
PmId
[1729206] Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius. (J Bacteriol. , 1992)
[7868593] Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995)
[8971703] Purification and characterization of the DNA-binding protein DnrI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius. (Mol Microbiol. , 1996)
[16545946] Feedback regulation of doxorubicin biosynthesis in Streptomyces peucetius. (Res Microbiol. , 2006)
[20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
comment
Blast 18th, id54%, 2e-63
Streptomyces peucetius_dnrI
Regulatory protein dnrI
[Adria_00210]transcriptional activator

--
[PMID: 1729206](1992)
ATCC 29050 strainでdnrI を不活化したら、RHO産生とdaunorubicin (DNR)産生ができなくなり、DNRへの抵抗性が著しく減った。

---
同じ著者らの続報。
[PMID: 7868593](1995)
dnrI::aphII mutantでは、DNR生合成におけるearly- and late-acting enzymesの両方をコードする転写物が存在しない。よってdnrI は、直接または間接的にDNR生合成遺伝子のすべてを調節している。

dnrI::aphII and dnrN::aphII strainsでresistance genes=drrAB and drrC転写物は消失。
よって、dnrI はDNR抵抗性の重大なregulatorであり、dnrNはdnrI を通して間接的にその影響を発現する、と示唆される。

--
[PMID: 8971703](1996) abstract
DNA-binding assays.
・dnrG-dpsABCD と dpsEF の間
・dnrC gene と dnrDKPSQ の間
の遺伝子間領域を含んだDNA断片へ特異的に結合する。

--
[PMID: 16545946](2006) abstract
dnrI promoter活性を増やすためにはDnrO and DnrN の両方が必要であった。

--
[PMID: 20529134]や[PMID: 20158521]で、dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。
dnrI promoterにはDnrNが結合し活性化される。

close this sectionSequence

selected fasta
>putative transcriptional regulator [AlnR3 SARP family regulator]
MEIEVLGPLDIRLDGTSIVPSAGKPRQILALLALRAGRIVPVPVLMEEIWGDRIPRSAQT
TLQTYILQLRRRISAARPDVRRPTAKDVLSTRFGGYLLSEPVLSSDVGTFQRLTAEGSAA
LERGEAGLAADVLGRALGLWHGSALIDVPTGHVLDTEILGIEEARARALELRIEADLRLG
RHAELLGELRMLVAQHPMHESFHAQLMIALCRSGHTWRALDVYQQLRSALVGELGVEPSD
RIQRLHQQVLGGGLDKPRSTYVERDLALELR
selected fasta
>putative transcriptional regulator [AlnR3 SARP family regulator]
ATGGAAATCGAAGTTTTAGGACCACTCGACATCCGTCTGGACGGCACGTCGATCGTGCCG
AGCGCGGGCAAACCTCGGCAGATACTGGCACTCCTCGCTCTGCGAGCCGGACGGATCGTA
CCCGTACCGGTTCTCATGGAGGAGATCTGGGGTGACCGAATACCCCGGAGCGCACAGACC
ACCTTGCAGACCTACATCCTTCAACTCCGCCGCAGGATCAGCGCAGCCCGTCCGGACGTC
CGACGACCGACCGCGAAGGACGTACTGTCCACCCGGTTCGGCGGATACCTGTTGTCCGAA
CCGGTGCTCAGCAGCGATGTCGGAACGTTCCAGCGACTCACGGCGGAAGGGAGCGCCGCA
CTGGAGAGGGGCGAGGCCGGCCTGGCCGCCGATGTGCTCGGCAGGGCCCTGGGCCTGTGG
CACGGCTCGGCGCTCATCGACGTACCGACGGGGCATGTCCTGGACACCGAGATTCTCGGC
ATCGAGGAGGCCAGGGCGCGGGCGCTCGAACTGCGGATCGAGGCCGATCTGCGACTCGGC
AGGCACGCCGAACTCCTCGGCGAGCTGCGGATGCTGGTAGCCCAGCATCCGATGCACGAG
AGCTTCCACGCGCAGCTGATGATCGCCCTGTGCCGTTCGGGGCACACCTGGCGGGCCCTC
GACGTCTATCAGCAACTGCGCTCGGCGCTCGTCGGGGAACTGGGCGTCGAACCGTCCGAC
CGGATCCAGCGGCTGCACCAGCAGGTCCTGGGCGGAGGCCTGGACAAACCGCGAAGCACC
TATGTGGAGCGCGATCTCGCCCTCGAACTGCGGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001867 Signal transduction response regulator, C-terminal (Domain)
[17-98] 1.8e-20 PF00486
PF00486   Trans_reg_C
[15-98] 2.00000075217457e-11 SM00862
SM00862   Trans_reg_C
IPR005158 Bacterial transcriptional activator domain (Domain)
[106-250] 7.10000000000008e-48 PF03704
PF03704   BTAD
[105-250] 4.60000044330862e-48 SM01043
SM01043   BTAD
IPR011990 Tetratricopeptide-like helical (Domain)
[103-226] 2.5e-05 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[24-100] 9.60000000000001e-09 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[1-99] 2.70000183580794e-13 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Alnu_00210 Alnu_00210   Alnu_00230 Alnu_00230
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