Alnu_00260 : CDS information

Location

Organism	Streptomyces sp.
Strain	CM020
Entry name	Alnumycin
Contig	EU852062
Start / Stop / Direction	22,454 / 23,746 / + [in whole cluster] 22,454 / 23,746 / + [in contig]
Location	22454..23746 [in whole cluster] 22454..23746 [in contig]
Type	CDS
Length	1,293 bp (430 aa)

Annotation

Category	5.1 general function
Product	putative two-component system histidine kinase
Product (GenBank)	AlnR4 two component sensory kinase
Gene
Gene (GenBank)	alnR4
EC number
Keyword
Note
Note (GenBank)
Reference	ACC B6SEH1 PmId [18940666] Characterization of the alnumycin gene cluster reveals unusual gene products for pyran ring formation and dioxan biosynthesis. (Chem Biol. , 2008) comment alnumycin gene clusterのクローニング、シークエンス。 aln2-alnT2の領域をS.albusに導入して、alnumycin産生されることを確認している。 AlnR4: Two-component sensory kinase 配列解析からの提唱。 AlnR4/AlnR5はtwo-component sensory kinase/response regulatorsのpair.
Related Reference	ACC O34757 PmId [15090506] The membrane fluidity sensor DesK of Bacillus subtilis controls the signal decay of its cognate response regulator. (J Bacteriol. , 2004) comment Blast 76th, id34%, 8e-23, N末イマイチ(膜貫通ドメインあり) B.subtilis_desK Sensor histidine kinase desK DesKのC末がautokinaseで、DesRにリン酸パスを行なっていることを示している。また、DesKのN末にある膜貫通領域がsenseに必須であるとも言っている。
Family/Domain	FD IPR011712 comment [IPR011712] Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain (Domain) function; GO:0000155 two-component sensor activity GO:0046983 protein dimerization activity

Sequence

>putative two-component system histidine kinase [AlnR4 two component sensory kinase]
MNDCGGRSMYAQGESYVVYAENRIHDYDHHYDYDSDGGEPGIGVELGATGLRLALFITCA
VQLGYALMAILNLLKWHHESMSLVGAVLILIAFFGLQLFHCNPYAVHLRARIGPWTLVPQ
AALAYAPFPMFGVLWGGFGGFLSGAVLVVLRGSVLAWVLFVLNAAAVCGLALASFALAPS
VYLTLATVVIGLMVYGLTRLSDIVVEQYRLRHRTALAAVSGERLRVARDLHDLLGYSLSA
ITLKSELTLRKVGVDDDRARKELVETLNIARQALADVRAVARGCRNLSVTAELRSVSGVL
DAADVETTIEGEPGDLDPGTGSVLAIVLREAVTNLLRHSAARHCRIEFGEADGQRWLAIS
NDGVEDGVSRTEAGRRGGGLGNLADRLGTVGGELNVTTEWGWFHLRAVLPARAAVPPPRP
GRQPTHHHAH

>putative two-component system histidine kinase [AlnR4 two component sensory kinase]
GTGAACGACTGCGGGGGTCGGTCCATGTATGCGCAGGGGGAAAGCTACGTGGTCTACGCA
GAGAATCGGATCCACGACTACGACCACCACTACGACTACGACTCCGACGGCGGTGAACCC
GGAATCGGAGTGGAATTAGGTGCGACCGGGCTGCGGCTGGCCCTGTTCATCACCTGCGCG
GTCCAGTTGGGCTACGCGCTGATGGCGATACTGAATCTGCTGAAGTGGCACCACGAATCC
ATGAGCCTCGTGGGCGCCGTGCTGATACTGATCGCATTCTTCGGGTTGCAGCTCTTTCAC
TGCAATCCCTATGCCGTTCATCTCAGGGCCAGGATCGGCCCCTGGACGCTCGTCCCGCAG
GCCGCACTGGCCTATGCGCCGTTTCCCATGTTCGGCGTGCTGTGGGGCGGCTTCGGCGGA
TTCCTCTCGGGGGCCGTGCTGGTCGTGCTCCGGGGGTCGGTGCTCGCCTGGGTCCTCTTC
GTCCTCAACGCCGCCGCCGTGTGCGGACTCGCCCTCGCCTCGTTCGCCCTCGCCCCGTCG
GTCTATCTGACGCTCGCCACCGTGGTCATCGGGCTGATGGTCTACGGGCTGACCCGGCTG
AGCGACATCGTCGTCGAGCAGTACAGGCTGCGTCACCGGACGGCCCTGGCCGCGGTCTCC
GGAGAGCGCCTCCGCGTCGCCCGCGACCTGCACGACCTGCTCGGCTACAGCCTCTCCGCC
ATCACGCTGAAAAGTGAACTGACCCTGCGGAAGGTGGGAGTCGACGACGACCGCGCCCGG
AAGGAACTGGTCGAGACGCTGAACATCGCACGGCAGGCACTGGCCGACGTCCGGGCCGTC
GCCCGCGGCTGCCGCAACCTGTCCGTCACGGCGGAGCTGCGCTCGGTGTCCGGAGTGCTG
GACGCGGCGGACGTGGAGACCACGATCGAGGGCGAGCCCGGTGACCTCGACCCGGGAACC
GGCTCGGTCCTGGCCATAGTGCTGCGGGAGGCCGTCACCAACCTGCTGCGGCACAGCGCG
GCGAGGCACTGCCGCATCGAGTTCGGCGAGGCCGACGGGCAGCGGTGGCTGGCGATCTCC
AACGACGGCGTCGAGGACGGGGTGTCACGCACCGAGGCCGGCCGCCGCGGCGGCGGCCTC
GGCAATCTCGCCGACCGGCTGGGTACCGTCGGCGGCGAGCTGAACGTCACCACCGAGTGG
GGCTGGTTCCATCTGCGCGCCGTGCTGCCGGCCCGGGCCGCCGTACCTCCACCCCGTCCC
GGGCGGCAGCCCACGCACCACCATGCACACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003594 ATPase-like, ATP-binding domain (Domain) SM00387 HATPase_c SSF55874 ATP_bd_ATPase PF02518 HATPase_c IPR011712 Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain (Domain) PF07730 HisKA_3
SignalP	Bacteria, Gram-negative
TMHMM	Transmembrane 5

Alnu_00250 Alnu_00270