Alip_00010 : CDS information

Location

Organism	Streptomyces aureofaciens
Strain	Tü117
Entry name	Alpha-lipomycin
Contig	DQ176871
Start / Stop / Direction	675 / 1 / - [in whole cluster] 675 / 1 / - [in contig]
Location	complement(1..675) [in whole cluster] complement(1..675) [in contig]
Type	CDS
Length	675 bp (224 aa)

Annotation

Category	4.1 transcriptional regulator
Product	two-component system response regulator
Product (GenBank)	LipReg1
Gene
Gene (GenBank)	lipReg1
EC number
Keyword
Note
Note (GenBank)	putative two-component system response regulator
Reference	ACC Q30CT4 PmId [16723573] Biosynthetic gene cluster for the polyenoyltetramic acid alpha-lipomycin. (Antimicrob Agents Chemother. , 2006) [19585113] Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tu117. (Appl Microbiol Biotechnol. , 2010) comment [PMID: 16723573](2006) alpha-lipomycin生合成遺伝子クラスターに関する文献。 lipReg1(224 aa): regulation lipReg1 自体の機能実験は行われておらず、two-component system response regulatorとの相同性が示されているのみ。 --- [PMID: 19585113](2010) alpha-lipomycin生合成に関与するregulatory networkに関する文献。 lipReg1挿入変異株ではalpha-lipomycinの生産が野生株と比較して低くなること、この変異株にlipReg1を相補するとalpha-lipomycinの生産が回復することが示されている。さらに、lipReg1変異株ではlipReg2, lipReg3, lipReg5の転写は野生株と変わらないが、lipReg4の発現が低くなることが示されている。また同様に、lipReg2変異株ではlipReg1, lipReg3, lipReg5の転写は野生株と変わらないが、lipReg4の発現が低くなることより、二成分制御系lipReg1, lipReg2によって、lipReg4の転写が調節されることが示唆されている。
Family/Domain	FD IPR001789, IPR000792 comment [IPR001789] Signal transduction response regulator, receiver domain (Domain) [IPR000792] Transcription regulator LuxR, C-terminal (Domain)

Sequence

>two-component system response regulator [LipReg1]
MTAIRLLLVDDDPLVRAGLSLMLGGADDVEIVGEAADGDEVGALVDRTRPDVVLMDIRMP
VVDGLTATERLRGRADAPQVVVLTTFHADEQVLRALRAGAAGFVLKDTPPAEILDAVRRV
AAGDPVLSPAVTRRLMEQAAGAAPDARRTRARARVDTLNDREREVAVAVGRGMANADIAT
DLFMSVATVKAHVSRILAKLDLNNRVQIALLVHDAGLLDEHADD

>two-component system response regulator [LipReg1]
ATGACCGCGATCAGACTGCTCCTCGTCGACGACGACCCGCTCGTGCGGGCCGGACTGTCC
CTCATGCTGGGCGGCGCGGACGACGTGGAGATCGTCGGTGAGGCCGCCGACGGCGACGAG
GTGGGGGCGCTCGTCGACCGCACCCGCCCGGACGTCGTCCTCATGGACATCCGGATGCCG
GTGGTGGACGGCCTCACGGCGACCGAGCGGCTGCGGGGCCGGGCGGACGCGCCCCAGGTC
GTCGTGCTCACCACCTTCCACGCGGACGAGCAGGTGCTGCGCGCCCTGCGGGCCGGTGCC
GCCGGCTTCGTCCTCAAGGACACCCCTCCCGCCGAGATCCTCGACGCGGTGCGCAGGGTC
GCCGCCGGAGACCCCGTCCTGTCGCCCGCCGTCACCCGGCGACTGATGGAGCAGGCGGCC
GGCGCCGCCCCCGACGCGAGGCGCACACGCGCGCGTGCCCGCGTGGACACCCTCAACGAC
CGCGAGCGCGAGGTCGCCGTCGCGGTCGGCCGCGGCATGGCCAACGCCGACATCGCGACC
GACCTCTTCATGAGCGTCGCCACGGTCAAGGCCCACGTCTCCCGCATCCTGGCCAAGCTC
GACCTCAACAACCGGGTCCAGATCGCCCTGCTGGTCCACGACGCCGGCCTGCTGGACGAG
CACGCCGACGACTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000792 Transcription regulator LuxR, C-terminal (Domain) PF00196 GerE SM00421 HTH_LUXR PR00038 HTHLUXR PS50043 HTH_LUXR_2 PS00622 HTH_LUXR_1 IPR001789 Signal transduction response regulator, receiver domain (Domain) PS50110 RESPONSE_REGULATORY SM00448 REC PF00072 Response_reg IPR011006 CheY-like superfamily (Domain) SSF52172 CheY_like IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	Eukaryota
TMHMM	No significant hit

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