Alip_00020 : CDS information

Location

Organism	Streptomyces aureofaciens
Strain	Tü117
Entry name	Alpha-lipomycin
Contig	DQ176871
Start / Stop / Direction	2,019 / 760 / - [in whole cluster] 2,019 / 760 / - [in contig]
Location	complement(760..2019) [in whole cluster] complement(760..2019) [in contig]
Type	CDS
Length	1,260 bp (419 aa)

Annotation

Category	5.1 general function
Product	two-component system histidine kinase
Product (GenBank)	LipReg2
Gene
Gene (GenBank)	lipReg2
EC number
Keyword
Note
Note (GenBank)	putative two-component system sensor kinase
Reference	ACC Q30CT3 PmId [16723573] Biosynthetic gene cluster for the polyenoyltetramic acid alpha-lipomycin. (Antimicrob Agents Chemother. , 2006) [19585113] Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tu117. (Appl Microbiol Biotechnol. , 2010) comment [PMID: 16723573](2006) alpha-lipomycin生合成遺伝子クラスターに関する文献。 lipReg2(419 aa): regulation lipReg2 自体の機能実験は行われておらず、two-component system sensor kinaseとの相同性が示されているのみ。 --- [PMID: 19585113](2010) alpha-lipomycin生合成に関与するregulatory networkに関する文献。 lipReg2 挿入変異株ではalpha-lipomycinの生産が野生株と比較して低くなること、この変異株にlipReg2を相補するとalpha- lipomycinの生産が回復することが示されている。さらに、lipReg2変異株ではlipReg1, lipReg3, lipReg5の転写は野生株と変わらないが、lipReg4の発現が低くなることが示されている。また同様に、lipReg1変異株では lipReg2, lipReg3, lipReg5の転写は野生株と変わらないが、lipReg4の発現が低くなることより、二成分制御系lipReg1, lipReg2によって、lipReg4の転写が調節されることが示唆されている。
Family/Domain	FD IPR011712 comment [IPR011712] Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain (Domain) Function GO:0000155 two-component sensor activity GO:0046983 protein dimerization activity

Sequence

>two-component system histidine kinase [LipReg2]
MSTVNSDKQATVPPAFAKRRWVLPAALVEDLYPEPRRPGGRPRRTARDWVVDFACFLLAV
AIGLLAADALDQEPDLSPAMAVADQLIGALACAAVWLRRRWPVGLAVAMVPVSLVSATAG
GAVLAALFTLAVHRPFRYVAWIGGTSLAVTPLYYWMRPEPGLPFLASLAIAFLLAVTVLG
WGMLVRSKRQLLLSLRDRARHAEAEARLRAEQAQRLAREAIAREMHDVLAHRLTLLSVHA
GALEFRPDAPREEVARAAGVIRESAHEALQDLRQIIGVLRAADSDDAGRPQPTLAALDAL
VAESRAAGMRVTLADRVPDPGAVPAAVGRTAYRIAQEALTNARKHAPGTEVTVSVTGAPG
AGLALSVRNAPPRGEVPHVPGSGQGLIGLTERATLAGGTLEHGPTPGGGFEVRARLPWG

>two-component system histidine kinase [LipReg2]
TTGTCGACTGTGAACAGTGACAAGCAGGCCACGGTGCCCCCGGCGTTCGCCAAGCGGCGC
TGGGTCCTGCCGGCGGCGCTGGTGGAGGACCTCTACCCGGAACCCCGGCGGCCCGGCGGC
CGGCCCCGGCGCACCGCACGCGACTGGGTCGTCGACTTCGCCTGCTTCCTGCTCGCGGTC
GCCATCGGCCTGCTGGCCGCCGACGCGCTGGACCAGGAACCGGACCTCTCACCCGCCATG
GCCGTCGCGGACCAGCTGATCGGCGCCCTCGCCTGCGCCGCCGTCTGGCTCCGGCGGCGC
TGGCCGGTCGGGCTGGCCGTGGCCATGGTGCCCGTCTCCCTGGTCTCGGCCACCGCGGGC
GGCGCCGTCCTCGCCGCCCTGTTCACGCTCGCCGTGCACCGGCCCTTCCGGTACGTGGCC
TGGATCGGCGGCACGAGTCTCGCCGTGACGCCCCTGTACTACTGGATGCGCCCGGAGCCC
GGGCTGCCCTTCCTCGCCTCCCTCGCCATCGCCTTCCTGCTCGCGGTCACTGTGCTCGGC
TGGGGCATGCTGGTCCGCTCCAAGCGCCAGCTCCTGCTCAGCCTGCGCGACCGCGCCCGG
CACGCCGAGGCCGAGGCGCGGCTGCGGGCCGAACAGGCGCAGCGGCTGGCCCGGGAGGCC
ATCGCCCGCGAGATGCACGACGTCCTCGCCCACCGGCTGACGCTGCTCAGCGTGCACGCG
GGCGCCCTGGAGTTCCGGCCCGACGCACCCCGCGAGGAGGTCGCGCGGGCGGCGGGCGTC
ATCCGGGAGAGCGCGCACGAGGCCCTCCAGGATCTGCGGCAGATCATCGGCGTCCTGCGG
GCGGCGGACTCCGACGACGCCGGCCGCCCCCAGCCGACGCTCGCGGCGCTCGACGCGCTG
GTCGCCGAGTCCCGTGCGGCCGGCATGAGGGTCACCCTCGCGGACCGCGTGCCCGACCCC
GGCGCCGTCCCCGCCGCCGTCGGCCGCACCGCGTACCGCATCGCCCAGGAGGCGCTCACG
AACGCGCGCAAGCACGCCCCCGGCACCGAGGTCACCGTCTCGGTCACCGGGGCGCCGGGC
GCCGGACTCGCCCTGTCGGTGCGCAACGCCCCGCCCCGGGGCGAGGTGCCCCACGTGCCC
GGTTCCGGCCAGGGCCTGATCGGGCTGACCGAGCGGGCCACGCTGGCGGGCGGCACCCTG
GAGCACGGCCCGACCCCCGGCGGCGGCTTCGAGGTGCGGGCGAGGCTGCCGTGGGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003594 ATPase-like, ATP-binding domain (Domain) SM00387 HATPase_c SSF55874 ATP_bd_ATPase PF02518 HATPase_c IPR011712 Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain (Domain) PF07730 HisKA_3
SignalP	Eukaryota Bacteria, Gram-negative
TMHMM	Transmembrane 5

Alip_00010 Alip_00030