Alip_00020 Alip_00020   Alip_00040 Alip_00040

Alip_00030 : CDS information

close this sectionLocation

Organism
StrainTü117
Entry nameAlpha-lipomycin
Contig
Start / Stop / Direction2,569 / 2,958 / + [in whole cluster]
2,569 / 2,958 / + [in contig]
Location2569..2958 [in whole cluster]
2569..2958 [in contig]
TypeCDS
Length390 bp (129 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative endoribonuclease
Product (GenBank)LipX1
Gene
Gene (GenBank)lipX1
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[16723573] Biosynthetic gene cluster for the polyenoyltetramic acid alpha-lipomycin. (Antimicrob Agents Chemother. , 2006)
[19585113] Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tu117. (Appl Microbiol Biotechnol. , 2010)
comment
[PMID: 16723573](2006)
alpha-lipomycin生合成遺伝子クラスターに関する文献。

lipX1(129 aa)
lipX1 自体の機能は予測されておらず、文献中ではguanyl-specific ribonucleaseとの相同性が示されているのみ。

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[PMID: 19585113](2010)
alpha-lipomycin生合成に関与するregulatory networkに関する文献。

lipX1の挿入変異株を作成し、変異株におけるalpha-lipomycin生産量が野生株よりも低くなることを確認している。また、lipX1を相補することによって、野生株よりもalpha-lipomycin生産量は増加する。
Related Reference
ACC
P30289
PmId
[9367812] ( , )
[12228255] ( , )
comment
<Guanyl-specific ribonuclease Sa3, Streptomyces aureofaciens, BLAST 9; id=60%>

---
[PMID: 9367812](1997)
Streptomyces aureofacienにおけるribonucleasesである Sa, Sa2, Sa3の遺伝子をクローニングして、E. coliで発現させて活性を調べている。

---
[PMID :12228255](2002)
Streptomyces aureofaciensにおけるRibonuclease (RNase) Sa3に関してのX線結晶構造解析を行った文献。
Family/Domain
FD
IPR000026
comment
[IPR000026] Guanine-specific ribonuclease N1/T1 (Family)

Function
GO:0003723 RNA binding
GO:0004521 endoribonuclease activity

close this sectionSequence

selected fasta
>putative endoribonuclease [LipX1]
MRFPPRITRIGTAAAVLSALLVGGTVATASPAAAAVGSICYGDLPSQAYDTLELIEQGGP
FPYSQDGTVFQNREGILPSQSSGYYHEYTVITPGSSTRGARRIVTGEENQEDYYTADHYE
SFDLVDYGC
selected fasta
>putative endoribonuclease [LipX1]
ATGAGATTCCCCCCACGAATCACTCGCATCGGCACCGCGGCCGCCGTCCTGTCCGCCCTC
CTCGTCGGAGGCACCGTCGCCACCGCCTCCCCGGCGGCCGCCGCGGTGGGCAGCATCTGC
TACGGCGACCTGCCCTCCCAGGCGTACGACACCCTCGAACTGATCGAGCAGGGCGGTCCC
TTCCCGTACTCGCAGGACGGCACCGTCTTCCAGAACCGGGAAGGCATCCTGCCGAGCCAG
TCCTCCGGCTACTACCACGAGTACACGGTGATCACGCCGGGCTCCTCCACGCGCGGTGCT
CGGCGCATCGTCACCGGCGAGGAGAACCAGGAGGACTACTACACGGCCGACCACTACGAG
TCCTTCGACCTGGTCGACTACGGCTGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000026 Guanine-specific ribonuclease N1/T1 (Family)
[44-124] 2.5e-25 PF00545
PF00545   Ribonuclease
IPR016191 Ribonuclease/ribotoxin (Domain)
[33-129] 6.60002751141562e-41 SSF53933
SSF53933   Ribonuclease/ribotoxin
[36-126] 2.50000000000001e-42 G3DSA:3.10.450.30
G3DSA:3.10.450.30   Ribonuclease/ribotoxin
SignalP
[1-29] 0.99 Signal
Eukaryota   
[1-34] 1 Signal
Bacteria, Gram-positive   
[1-34] 1 Signal
Bacteria, Gram-negative   
TMHMM
[13-35] Transmembrane (i-o)
Transmembrane 1   
Alip_00020 Alip_00020   Alip_00040 Alip_00040
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