Alip_00190 Alip_00190   Alip_00210 Alip_00210

Alip_00200 : CDS information

close this sectionLocation

Organism
StrainTü117
Entry nameAlpha-lipomycin
Contig
Start / Stop / Direction64,303 / 63,320 / - [in whole cluster]
64,303 / 63,320 / - [in contig]
Locationcomplement(63320..64303) [in whole cluster]
complement(63320..64303) [in contig]
TypeCDS
Length984 bp (327 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 ketoreductase
Product (GenBank)LipDig3
Gene
Gene (GenBank)lipDig3
EC number
Keyword
  • D-digitoxose
  • axial-OH
Note
Note (GenBank)
  • putative 3-ketoreductase
Reference
ACC
PmId
[16723573] Biosynthetic gene cluster for the polyenoyltetramic acid alpha-lipomycin. (Antimicrob Agents Chemother. , 2006)
comment
alpha-lipomycin生合成遺伝子クラスターに関する文献。

lipDig3(327 aa): 3-ketoreductase

lipDig3 自体の機能実験は行われていないが相同性より、D-digitoxose moietyを生産する際の
NDP-3,4-diketo-2,6-dideoxy-D-glucose → NDP-4-keto-2,6-dideoxy-D-allose
へと変換する反応を司ると予想している。
Related Reference
ACC
Q9XC70
PmId
[16448097] A two-stage one-pot enzymatic synthesis of TDP-L-mycarose from thymidine and glucose-1-phosphate. (J Am Chem Soc. , 2006)
comment
<NDP-hexose 2,3-enoyl reductase TylCII (TylC1), Streptomyces fradiae, BLAST 23; id=59%>

--
[PMIDなし]Biosynthesis of Mycarose: Isolation and Characterization of Enzymes Involved in the C-2 Deoxygenation. J Am Chem Soc 1999

TylX3をdTDP-D-glucoseを4,6-dehydrataseで処理した基質と反応するとmaltol産生。これは予測された不安定なdehydration産物の分解の結果であると示唆される。

TylX3にTylC1, NADPHを加えて反応すると、C-3 OH基がaxialなdTDP-2,6-dideoxy-D-glycero-D-glycero-4-hexuloseを産生。
この産物のC-2のHはequatrial positionであることが重水素の取り込みで確認されたので、C-2の立体配置は保持されており、水素化物がNADPHから不安定中間体のC-3 positionへ移されることがほのめかされる。

--
[PMID:16448097]上記論文の引用あり。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 ketoreductase [LipDig3]
MHYTRLGRTALKISRLCVGTVNFGGRVDEEDGHRILDRALDLGLNFVDTANMYGWRVHKG
YTEETIGRWFAKDPSCRDRTVLATKVGNEMSGAPNQSGLSARHIVASCEASLRRLQTDRI
DLFQLHHVDRTAPWDEIWQALDLLVSQGKVLYVGSSNFAGWHVAMAQEAAGRRHFLGLVS
EQCAYNLVTRLPELELIPAARAYGMAVLPWSPLHGGLLGGVLAKLREGSAVKSAQGRAVP
ALEEHRGRIEAYEELCRELGEHPAQVGLAWVLSRPGVTAPVIGPRSVEQLDSAVAALDLT
LPHDALTRLDALFPGPRAEAPEAWITT
selected fasta
>putative NDP-hexose C-3 ketoreductase [LipDig3]
ATGCACTACACACGTCTGGGCCGCACCGCACTGAAGATCAGCAGATTGTGCGTGGGAACG
GTCAACTTCGGCGGACGGGTGGACGAGGAGGACGGGCACCGCATCCTGGACCGCGCCCTC
GACCTGGGCCTCAACTTCGTCGACACCGCCAACATGTACGGCTGGCGCGTGCACAAGGGC
TATACGGAGGAGACGATCGGCCGCTGGTTCGCCAAGGACCCGTCCTGCCGGGACCGGACC
GTGCTGGCCACCAAGGTCGGCAATGAGATGTCCGGCGCGCCCAACCAGAGCGGTCTGTCG
GCCCGACACATCGTCGCGTCCTGCGAGGCGTCCCTGCGCCGACTGCAGACCGACCGCATC
GACCTCTTCCAACTGCACCACGTGGACCGCACGGCGCCCTGGGACGAGATCTGGCAGGCA
CTGGACCTGCTGGTGAGCCAGGGAAAGGTGCTGTACGTGGGCTCGTCCAACTTCGCGGGC
TGGCACGTGGCCATGGCCCAGGAGGCGGCCGGCCGGCGGCACTTCCTCGGCCTGGTCTCC
GAGCAGTGCGCGTACAACCTGGTCACCCGCCTGCCCGAGCTGGAACTCATCCCCGCCGCG
CGCGCCTATGGCATGGCCGTGCTGCCGTGGTCGCCACTGCACGGCGGACTGCTCGGCGGG
GTCCTCGCCAAGCTGCGCGAGGGGAGCGCGGTCAAGTCGGCGCAGGGGCGGGCGGTGCCG
GCTCTGGAGGAGCACCGCGGCCGTATCGAAGCCTACGAAGAGCTGTGCCGTGAGCTGGGC
GAGCACCCCGCTCAGGTCGGCCTCGCCTGGGTGCTGTCGCGTCCGGGGGTGACCGCTCCC
GTCATCGGCCCGCGCAGTGTCGAGCAGTTGGACAGCGCGGTCGCCGCGTTGGACCTCACT
CTGCCGCACGATGCTCTGACCCGTCTCGACGCGTTGTTCCCGGGGCCGCGCGCGGAGGCA
CCGGAGGCGTGGATCACGACATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR023210 NADP-dependent oxidoreductase domain (Domain)
[1-321] 2.7000136937899e-91 SSF51430
SSF51430   Aldo/ket_red
[1-314] 3.79999999999991e-88 G3DSA:3.20.20.100
G3DSA:3.20.20.100   Aldo/ket_red
[15-313] 3.49999999999995e-69 PF00248
PF00248   Aldo_ket_red
SignalP No significant hit
TMHMM No significant hit
Alip_00190 Alip_00190   Alip_00210 Alip_00210
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