Avil_00020 Avil_00020   Avil_00040 Avil_00040

Avil_00030 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction3,148 / 3,402 / + [in whole cluster]
3,148 / 3,402 / + [in contig]
Location3148..3402 [in whole cluster]
3148..3402 [in contig]
TypeCDS
Length255 bp (84 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative toxin
Product (GenBank)AviX3
GeneaviX3
yoeB
Gene (GenBank)aviX3
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in avilamycin biosynthesis.
Note (GenBank)
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
comment
avilamycin A生合成遺伝子クラスターの報告。

AviX3: Protein of unknown function from S. coelicolor A3(2) [Proposed function:Unknown]

avi gene clusterの境界決定のための実験をしていないが、AviRb-AviZ2が生合成遺伝子だと記述している。
Related Reference
ACC
Q9Z4V8
PmId
comment
Streptomyces coelicolor
Toxin YoeB(SCO2236)

Streptomyces lividans and S. coelicolorのYefM/YoeB familyに属するTA systemの機能解析している。
S. lividans antitoxin (YefMsl), toxin (YoeBsl)の精製とTA complex(YefM/YoeBsl)はbicistronic operonのプロモーター領域へこの複合体が結合することを示した。In vivo実験でtoxin YoeBslは開始コドン下流の3塩基でmRNAを切り出すことで転写開始を抑制した。
S. coelicolorのSCOs2235(yefMsc),2236(YoeBsc)でも同様な結果が得られた。

Avil_00020がSCO2235(yefM)に相当する。

close this sectionSequence

selected fasta
>putative toxin [AviX3]
MRLVFEDQGWDDYTSWLKNDRKMLARINKLIEDVRRDPFTGIGKPEPLKYHLPGAWSRRI
DDEHRLVYLVTDKEIVILAARYHY
selected fasta
>putative toxin [AviX3]
GTGAGGCTTGTCTTCGAGGATCAGGGCTGGGATGACTACACGTCCTGGCTCAAGAACGAC
CGCAAGATGCTCGCCCGCATCAACAAGCTCATCGAGGACGTCAGGCGCGACCCCTTCACG
GGGATCGGCAAACCCGAGCCGCTGAAGTACCACTTGCCGGGGGCGTGGTCGCGGCGGATC
GACGACGAACACCGCCTCGTGTACCTGGTTACGGACAAGGAGATCGTGATCCTCGCTGCC
CGGTACCACTACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009614 Addiction module toxin, Txe/YoeB (Family)
[5-84] 4.2e-39 TIGR02116
TIGR02116   Toxin_Txe_YoeB
[5-84] 1.5e-41 PF06769
PF06769   Plasmid_Txe
SignalP No significant hit
TMHMM No significant hit
Avil_00020 Avil_00020   Avil_00040 Avil_00040
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