Avil_00010 Avil_00010   Avil_00030 Avil_00030

Avil_00020 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction2,870 / 3,151 / + [in whole cluster]
2,870 / 3,151 / + [in contig]
Location2870..3151 [in whole cluster]
2870..3151 [in contig]
TypeCDS
Length282 bp (93 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative antitoxin
Product (GenBank)AviX2
GeneaviX2
yefM
Gene (GenBank)aviX2
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in avilamycin biosynthesis.
Note (GenBank)
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
comment
avilamycin A生合成遺伝子クラスターの報告。

AviX2: Protein of unknown function from S. coelicolor A3(2) [Proposed function:Unknown]

avi gene clusterの境界決定のための実験をしていないが、AviRb-AviZ2が生合成遺伝子だと記述している。
Related Reference
ACC
Q9Z4V7
PmId
comment
Streptomyces coelicolor
Antitoxin YefM(SCO2235)

Streptomyces lividans and S. coelicolorのYefM/YoeB familyに属するTA systemの機能解析している。
S. lividans antitoxin (YefMsl), toxin (YoeBsl)の精製とTA complex(YefM/YoeBsl)はbicistronic operonのプロモーター領域へこの複合体が結合することを示した。In vivo実験でtoxin YoeBslは開始コドン下流の3塩基でmRNAを切り出すことで転写開始を抑制した。
S. coelicolorのSCOs2235(yefMsc), 2236(YoeBsc)でも同様な結果が得られた。

Avil_00030がSCO2236(YoeB)に相当する。

close this sectionSequence

selected fasta
>putative antitoxin [AviX2]
MSINRERSRKALFPLIKKVNDNHEAIEIVSKHGNAVLVSAEDYAALREGSYLLRSPANAR
RLLKAYENALAHVNVSERELIDPDSADAGSGAA
selected fasta
>putative antitoxin [AviX2]
ATGTCGATAAACCGCGAGCGAAGCCGCAAGGCTCTCTTTCCGCTGATCAAGAAGGTCAAC
GACAATCACGAGGCCATCGAGATCGTCTCCAAGCACGGCAACGCCGTACTCGTCTCGGCC
GAGGATTATGCAGCGCTGCGCGAGGGCTCGTACCTGCTGCGCTCTCCGGCGAACGCCCGT
CGACTGCTCAAGGCGTACGAGAACGCCCTTGCCCACGTCAATGTGTCGGAGCGGGAGCTG
ATCGATCCGGATTCGGCGGACGCTGGTTCGGGTGCCGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006442 Type II toxin-antitoxin system, antitoxin Phd/YefM (Family)
[13-64] 5.5e-10 PF02604
PF02604   PhdYeFM_antitox
[12-54] 2.8e-07 TIGR01552
TIGR01552   Phd_fam
SignalP No significant hit
TMHMM No significant hit
Avil_00010 Avil_00010   Avil_00030 Avil_00030
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