Avil_00130 Avil_00130   Avil_00150 Avil_00150

Avil_00140 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction11,680 / 11,060 / - [in whole cluster]
11,680 / 11,060 / - [in contig]
Locationcomplement(11060..11680) [in whole cluster]
complement(11060..11680) [in contig]
TypeCDS
Length621 bp (206 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
ProductLuxR family transcriptional activator
Product (GenBank)putative two-component regulator
Gene
Gene (GenBank)aviC1
EC number
Keyword
Note
Note (GenBank)
  • AviC1
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
[19609289] Production of avilamycin A is regulated by AviC1 and AviC2, two transcriptional activators. (J Antibiot (Tokyo). , 2009)
comment
[PMID:11410376]
avilamycin A生合成遺伝子クラスターの報告。

AviC1: Putative two-component response regulator from S. aureus [Proposed function:Regulation]

このORFの機能解析はされていない。


[PMID:19609289]
AviC1とAviC2の機能解析

methyltransferase gene aviG4欠損株GW4でそれぞれ作製したaviC1, aviC2不活性化株は、両株ともgavibamycin A誘導体あるいはその中間体を生産しなかった。

RT-PCRによる転写解析結果から、aviC1, aviC2は構造遺伝子の発現を制御する事によりavilamycinの生産をpositiveに制御すると考察。aviC1がaviC2の発現を制御しており、aviC2は自己調節因子として機能するかもしれないと考察している。

AviC1, AviC2は既知のSARPやLAL familyの制御因子とは異なるとも言っている。

close this sectionSequence

selected fasta
>LuxR family transcriptional activator [putative two-component regulator]
MPCTHRARAALSALPTPRRALLTTAGGTKAPQPGRRCDKMIKMSNVQDPFVAWVVSPSRL
HSSVVAPFGALILDASDDLLGHARTTRSYGGQTLPYSGCDSSDSGDDRFLHEDQFPWLEG
LSQVHLLSDRELQVFLLLSHGYSNREIAALLGVTERTVKAHMAQILAKLGVESRLQAGLV
AFAHGLPGHIGDAEGSDHPPDTSAGS
selected fasta
>LuxR family transcriptional activator [putative two-component regulator]
TTGCCGTGCACACACCGGGCACGGGCCGCCTTATCCGCCTTGCCCACGCCTCGGCGGGCC
CTGCTCACGACAGCAGGCGGCACCAAGGCGCCCCAACCCGGCCGTAGATGCGATAAGATG
ATCAAGATGAGTAATGTCCAGGACCCATTCGTCGCATGGGTGGTATCGCCCTCACGTCTT
CATAGTTCAGTGGTGGCGCCCTTCGGCGCCTTGATACTCGATGCGTCGGATGACCTCTTA
GGGCATGCAAGGACCACACGCTCATACGGGGGCCAGACTTTGCCATATTCCGGCTGCGAT
TCCAGTGATTCTGGCGACGATCGATTTCTCCACGAGGACCAGTTCCCCTGGCTGGAAGGG
CTCAGCCAGGTACATTTACTCTCCGACCGCGAGCTCCAGGTCTTCCTCCTGCTGAGCCAC
GGCTACTCCAACCGTGAGATCGCCGCGCTGCTCGGGGTCACCGAGCGCACCGTCAAGGCT
CATATGGCCCAGATACTGGCGAAGCTCGGGGTCGAATCCCGTCTTCAGGCCGGACTGGTT
GCCTTCGCCCACGGGCTTCCCGGACACATCGGCGATGCAGAGGGATCCGACCATCCACCC
GATACGTCCGCGGGTTCCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[120-185] PS50043
PS50043   HTH_LUXR_2
[124-181] 1.20000117458134e-24 SM00421
SM00421   HTH_LUXR
[127-141] 1.09999909120787e-12 PR00038 [141-157] 1.09999909120787e-12 PR00038 [157-169] 1.09999909120787e-12 PR00038
PR00038   HTHLUXR
[126-178] 3.1e-16 PF00196
PF00196   GerE
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[123-186] 5.30000000000001e-21 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[105-191] 3.1999989904635e-20 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP
[1-20] 0.776 Signal
Bacteria, Gram-negative   
[1-22] 0.637 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Avil_00130 Avil_00130   Avil_00150 Avil_00150
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