Avil_00140 Avil_00140   Avil_00160 Avil_00160

Avil_00150 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction12,529 / 11,951 / - [in whole cluster]
12,529 / 11,951 / - [in contig]
Locationcomplement(11951..12529) [in whole cluster]
complement(11951..12529) [in contig]
TypeCDS
Length579 bp (192 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
ProductLuxR family transcriptional activator
Product (GenBank)putative response regulator
Gene
Gene (GenBank)aviC2
EC number
Keyword
Note
Note (GenBank)
  • AviC2
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
[19609289] Production of avilamycin A is regulated by AviC1 and AviC2, two transcriptional activators. (J Antibiot (Tokyo). , 2009)
comment
[PMID:11410376]
avilamycin A生合成遺伝子クラスターの報告。

AviC2: Putative response regulator from S. noursei [Proposed function:Regulation]

このORFの機能解析はされていない。

[PMID:19609289]
AviC1とAviC2の機能解析

methyltransferase gene aviG4欠損株GW4でそれぞれ作製したaviC1, aviC2不活性化株は、両株ともgavibamycin A誘導体あるいはその中間体を生産しなかった。

aviC1，aviC2の過剰発現株におけるgavibamycin A1とgavibamycin A3の生産量を比較。AviC2は生産株の生理学的状態に依存したavilamycin遺伝子の正確な発現により、avilamycin生合成を制御するかもしれないと考察している。

さらに、RT-PCRによる転写解析結果から、aviC1,aviC2は構造遺伝子の発現を制御する事によりavilamycinの生産をpositiveに制御すると考察。aviC1がaviC2の発現を制御しており、aviC2は自己調節因子として機能するかもしれないと考察している。

AviC1, AviC2は既知のSARPやLAL familyの制御因子とは異なるとも言っている。

close this sectionSequence

selected fasta
>LuxR family transcriptional activator [putative response regulator]
MPVTRGFAAGTGTPQRGMRPLHPGAPQLPSRRSLLRTAMIVKSGQPAAGPRNPPHLLEQP
NVQEPRPAPERKSTTAGERVLGTKESAAGAWSRPGALDTSQHSSPREYQVRTAPTTLSSL
DAKIIERIAAGERTTCIASKLYLSRQGVEYHVSKMMERFRVPNRTALVAKAYALGILRSE
VWPPRVLQERVN
selected fasta
>LuxR family transcriptional activator [putative response regulator]
ATGCCTGTTACCCGCGGTTTCGCGGCCGGAACGGGCACGCCACAGCGCGGGATGCGGCCG
TTACACCCCGGAGCGCCTCAACTACCGAGCCGTCGCAGCCTCTTGCGGACCGCCATGATC
GTGAAGTCCGGTCAGCCGGCCGCAGGCCCCCGAAACCCGCCCCATTTGCTCGAACAACCC
AATGTCCAAGAACCGCGACCGGCACCTGAACGAAAGTCCACTACTGCTGGTGAACGAGTG
TTGGGTACAAAGGAGTCAGCCGCCGGTGCCTGGTCCCGCCCCGGCGCTCTCGACACCTCT
CAGCACTCTTCCCCCAGGGAGTACCAGGTGAGAACCGCCCCCACGACCTTATCCAGCCTG
GACGCCAAGATCATCGAACGCATCGCCGCAGGTGAGCGCACCACCTGCATCGCCAGCAAG
CTCTACCTCAGCCGCCAGGGGGTCGAGTACCACGTCAGCAAGATGATGGAGCGCTTCCGG
GTGCCCAACCGCACCGCCCTCGTCGCCAAGGCCTACGCGCTGGGAATCCTGAGATCCGAG
GTGTGGCCCCCCAGGGTTCTGCAGGAACGCGTCAATTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[116-171] 5.90000000000001e-10 PF00196
PF00196   GerE
[114-171] 2.00000075217457e-06 SM00421
SM00421   HTH_LUXR
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[107-177] 4.1e-12 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[95-177] 5.29999657550189e-11 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Avil_00140 Avil_00140   Avil_00160 Avil_00160
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