Avil_00270 Avil_00270   Avil_00290 Avil_00290

Avil_00280 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction27,779 / 28,792 / + [in whole cluster]
27,779 / 28,792 / + [in contig]
Location27779..28792 [in whole cluster]
27779..28792 [in contig]
TypeCDS
Length1,014 bp (337 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductUDP-glucuronic acid decarboxylase
Product (GenBank)putative NDP-glucose 4,6-dehydratase
Gene
Gene (GenBank)aviE2
EC number
Keyword
  • 2-O-isobutyryl-L-lyxose
Note
Note (GenBank)
  • AviE2
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
[16242656] Genes encoding enzymes responsible for biosynthesis of L-lyxose and attachment of eurekanate during avilamycin biosynthesis. (Chem Biol. , 2005)
comment
[PMID:11410376]
Avilamycin生合成遺伝子クラスターの報告。

AviE2: dTDP-glucose 4,6-dehydratase from S. coelicolor A(3)2 [Proposed function:Sugar biosynthesis]

このORFの機能解析はされていない。


[PMID:16242656(2005)]
AviD,AviE2の機能解析の報告。

AviE2はeurekanate生合成に関与するNDP-glucose 4,6-dehydrataseではなく、UDP-L-lyxose形成に関与するUDP-glucuronic acid(UDP-GlcA) decarboxylaseであることを示した。

AviE2の配列はshort chain dehydrogenase/reductase superfamilyに属する、UDP-sugarsのC-4 positionを酸化する酵素がもつ保存モチーフや保存アミノ酸を全て保存していた。
aviE2の不活性株は、avilamycin誘導体を形成しなかった。
大腸菌で発現・精製し、UDP-GlcA decarboxylaseの特徴を示した。また、HPLC-MS,NMRで解析し反応産物はUDP-D-xyloseであった。反応速度論的解析より基質はNAD+特異的なUDP-GlcAであることを示した。

close this sectionSequence

selected fasta
>UDP-glucuronic acid decarboxylase [putative NDP-glucose 4,6-dehydratase]
MTIGLKKGQRAVVTGGAGFIGSHLCERLIERGLSVTCVDNLSTGRIANLDALADEERFTL
LRADVTEPFSVEGPVHHVVHLASPASPLDYLALPLETLRVGSAGTENALRLAVAHGARFV
VASTSEIYGDPAEHPQSESYWGNVNPIGPRSVYDEAKRFTEALTAAYARTLGADTGIARL
FNSYGPRMRRDDGRVVPTFIDQALAGLPLTINGSGAQTRSLCYVEDTVRGLMALMDSSFP
GPVNIGATGEMTVRQIAEAIAALAGVELRTEFRAPAEDEPGRRCPDIETARTKLGWKPEV
PLAEGLKRTLLWWESTYPRDMAHDGVNGSSDIRSGAA
selected fasta
>UDP-glucuronic acid decarboxylase [putative NDP-glucose 4,6-dehydratase]
GTGACAATCGGGCTGAAGAAGGGACAGCGGGCCGTCGTCACCGGGGGTGCCGGCTTCATC
GGCTCCCACCTGTGCGAACGGCTCATCGAGCGGGGTCTGTCGGTGACCTGCGTGGACAAC
CTGTCCACCGGCAGGATCGCGAACCTCGACGCGCTCGCCGACGAGGAGCGGTTCACGCTG
CTGCGGGCCGACGTGACCGAGCCGTTCTCCGTCGAGGGGCCGGTCCACCACGTCGTCCAC
CTGGCGTCGCCGGCCTCCCCCCTGGACTATCTGGCGCTGCCGCTGGAGACGCTGCGGGTC
GGCTCGGCCGGGACCGAGAACGCGCTGCGGCTGGCGGTGGCGCACGGCGCCCGCTTCGTG
GTCGCCTCCACCTCGGAGATCTACGGGGATCCCGCCGAGCACCCGCAGTCCGAGAGCTAC
TGGGGCAACGTCAACCCGATCGGCCCGCGTTCGGTGTACGACGAGGCCAAACGGTTCACC
GAGGCGCTGACCGCCGCCTACGCGCGCACGCTGGGCGCGGACACCGGCATTGCCCGCCTG
TTCAACTCCTACGGACCGCGGATGCGCCGCGACGACGGGCGGGTGGTGCCGACCTTCATC
GACCAGGCGCTCGCCGGACTGCCGCTCACCATCAACGGCAGCGGCGCCCAGACCCGGTCG
CTGTGCTACGTCGAGGACACGGTGCGGGGACTGATGGCCCTGATGGACTCGTCCTTCCCG
GGCCCGGTCAACATCGGCGCGACCGGTGAGATGACCGTCCGTCAGATCGCGGAGGCGATC
GCCGCACTCGCGGGAGTGGAGCTGCGGACCGAGTTCCGCGCGCCGGCGGAGGACGAGCCC
GGCCGGCGCTGCCCCGACATCGAGACCGCCAGGACGAAGCTGGGCTGGAAGCCCGAGGTG
CCGCTCGCCGAAGGGCTCAAGCGCACCCTGCTCTGGTGGGAGAGCACCTACCCGCGGGAC
ATGGCCCACGACGGCGTGAACGGTTCGAGCGACATCAGGAGTGGAGCGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[12-246] 1.40000000000001e-44 PF01370
PF01370   Epimerase
IPR016040 NAD(P)-binding domain (Domain)
[7-180] 4.9e-41 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Avil_00270 Avil_00270   Avil_00290 Avil_00290
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